Anti-Mouse PD1 Monoclonal Antibodies for Flow Cytometry

PA007505.r2a: Recombinant Anti-mouse PD1 Monoclonal Antibody (Clone: 29F.1A12.1), Rat IgG2a Kappa

Recombinant Rat IgG2a Monoclonal Antibody. 
Clone: 29F.1A12.1.
Isotype:  Rat IgG2a Kappa.
Source: The anti-mouse PD1 monoclonal antibody (clone: 29F.1A12.1) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 29F.1A12.1 antibody specifically binds to mouse PD1 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007505.h1Fs: Recombinant Anti-mouse PD1 Monoclonal Antibody (Clone: 29F.1A12.1), Human IgG1 Fc Silent Kappa

Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: 29F.1A12.1.
Isotype: Human IgG1 kappa.
Source: The anti-mouse PD1 monoclonal antibody (clone: 29F.1A12.1) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 29F.1A12.1 antibody specifically binds to mouse PD1 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The 29F.1A12.1 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.

Background
The rat anti-mouse PD-1 monoclonal antibody 29F.1A12 (rat IgG2a kappa) reacts with the mouse PD-1 protein (programmed death-1 or CD279) encoded by the mouse Pdcd1 gene, a member of the CD28 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2, both of which belong to the B7 family. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD-1 and anti-mouse PD-L1 antibodies which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The 29F.1A12 monoclonal antibody blocks the binding of both the mouse PD-L1 protein and the mouse PD-L2 protein to the mouse PD-1 protein.
Our recombinant 29F.1A12 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse PD-1 monoclonal antibody (hybridoma clone name or number: 29F.1A12).

References of anti-mouse PD-1 antibody (29F.1A12) for Flow Cytometry

Anti-PD-1 therapy triggers Tfh cell–dependent IL-4 release to boost CD8 T cell responses in tumor-draining lymph nodes.
Ruggiu, M., et al. (2024). J Exp Med. 2024 Apr 1;221(4):e20232104. doi: 10.1084/jem.20232104. PMID: 38417020
Lymph node cells were stained ex vivo with a different anti-PD-1 mAb clone that did not interfere with the injected mAb ( Polesso et al., 2021) to link in vivo labeling with PD-1 surface expression levels. ...(B) Representative FACS dot plot showing in vivo labeling by AF594-labeled anti-PD-1 mAb (clone RMP1-14) and ex vivo staining by anti-PD-1 antibody (clone 29F.1A12). ...anti-mouse PD1-BX036 (purified clone 29F.1A12 from...
Tags: anti-mouse PD-1 (CD279) (29F.1A12); activity of 29F.1A12 antibody

Distinct antibody clones detect PD-1 checkpoint expression and block PD-L1 interactions on live murine melanoma cells.
Martins, C., et al. (2022). Sci Rep. 2022 Jul 21;12(1):12491. doi: 10.1038/s41598-022-16776-1. PMID: 35864188
Similarly, in preclinical cancer models, several PD-1 blocking ab clones, including 29F.1A12 (anti-mouse PD-1 antibody), thwart tumor progression 4 , 5 , 6 , 7 , 8. ...For example, TME-infiltrating macrophages and natural killer (NK) cells both express PD-1, the blockade of which by 29F.1A12 (anti-mouse PD-1 mAb) contributes to tumor growth suppression and prolonged survival 10 , 11. ...29F.1A12 antibody recognizes PD-1 on B16-F10 and other murine tumor lines, suppresses cancer cell-intrinsic PD-1 receptor signaling, and inhibits resultant tumor growth in three-dimensional (3D) culture and tumor-bearing mice 5 , 7...
Tags: function of 29F.1A12 antibody; binding to mouse PD-1 (mPD-1)

Dying cells expose a nuclear antigen cross-reacting with anti-PD-1 monoclonal antibodies.
Metzger, P., et al. (2018). Sci Rep. 2018 Jun 13;8(1):9040. doi: 10.1038/s41598-018-27125-6. PMID: 29892077
In addition, PD-1 staining (clone 29F1A12) uncovered a FSC-H low population predominantly positive for PD-1. ...PD-1 (clone 29F.1A12) and fixable viability dye (FVD) staining was assessed by flow cytometry. ...PD-1 staining (clone 29 F.1A12) in these cells could be due to binding of an intracellular PD-1 pool that becomes accessible in cells with leaky membranes...Again, PD-1 mAb staining (clone 29F.1A12) was found in both PD-1 wild-type and knock-out cells in the dead cell fraction, indicative of unspecific binding. ...We assessed PD-1 expression by flow cytometry and compared the two mainly used antibody clones (29 F.1A12 and RMP1-14), which were also included in the above-mentioned study.
Tags: PD 1 (CD279) antibody, anti-mouse; rat anti-mouse PD-1 (CD279)

Adaptive resistance to therapeutic PD-1 blockade is associated with upregulation of alternative immune checkpoints.
Koyama, S., et al. (2016). Nat Commun. 2016 Feb 17;7:10501. doi: 10.1038/ncomms10501. PMID: 26883990
Fluorescent conjugated anti-PD-1 antibody is the same clone (29F.1A12) as the therapeutic antibody. ...PD-1-blocking antibody (clone 29F.1A12), TIM-3-blocking antibody (clone RMT3–23) and their isotype controls (clone 2A3) were injected intraperitoneally into the mice for therapeutic treatment (three times a week, 200 μg for PD1 and 100 μg for TIM-3 per dose).
Tags: mouse PD-1-specific antibody; 29F.1A12 mAb for flow cytometry

The PD-1 Pathway Regulates Development and Function of Memory CD8+ T Cells following Respiratory Viral Infection.
Pauken, K. E., et al. (2020). Cell Rep. 2020 Jun 30;31(13):107827. doi: 10.1016/j.celrep.2020.107827. PMID: 32610128
WT B6 mice were treated with anti-PD-1 antibody (clone 29F.1A12) or isotype control antibody (rat IgG2a) on days −1, 2, and 5 and harvested on day 8 p.i., or treated on days −1, 2, 5, and 8 and harvested on day 60+ p.i. PD-L1/L2 DKO mice were included as a control....For in vivo antibody blockade experiments, WT C57BL/6 mice were given 200 μg per mouse per injection of rat anti-PD-1 antibody (clone 29F.1A12) or isotype control antibody (Rat IgG2a, clone 2A3) on days −1, 2, and 5 p.i. and analyzed at day 8 p.i., or on days −1, 2, 5, and 8 p.i. and analyzed at day 60+ p.i...
Tags: anti-mouse PD-1 antibody for flow cytometry; 29F.1A12 mAb for flow cytometry

For more references about anti-mouse PD-1 antibody (clone 29F.1A12) for flow cytometry, please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following anti-mouse PD-L1 / PD-1 antibodies:
In vivo grade recombinant anti-mouse PD1 antibodies (Clone 29F.1A12.1)
In vivo grade recombinant anti-mouse PD-1 antibodies (Clone RMP1-14.1)
In vivo grade recombinant anti-mouse PD-L1 antibodies (Clone 10F.9G2.1)

Recombinant PD1 and PDL1 Proteins:
Recombinant Human PD-1/PDCD1 Proteins
Biotinylated Recombinant Human PD-1/PDCD1 Proteins
Recombinant Mouse PD-1/PDCD1 Proteins
Biotinylated Recombinant Mouse PD-1/PDCD1 Proteins
Recombinant Cynomolgus PD-1/PDCD1 Proteins
Recombinant Canine PD-1/PDCD1 Proteins
Biotinylated Cynomolgus/Rhesus macaque PD-L1 Protein
Biotinylated Human PD-L1 Protein
Biotinylated Mouse PD-L1 Protein
Cynomolgus PD-L1 Protein
Cynomolgus/Rhesus macaque PD-L1 Protein
Human PD-L1/B7-H1 Protein
Mouse PD-L1/B7-H1 Protein