100000 cells per hybridoma clone are sufficient for cloning and sequencing the monoclonal antibody variable regions (both VH and VL) and full length. Welcome to challenge us with smaller number of clonal B cells or non-viable cells for hybridoma rescue.

- Reliable results. With optimized workflow and PCR primers, we have successfully cloned and sequenced >3000 antibodies from various species in the past 10 years. Syd Labs has become a reliable CRO supplier of antibody sequencing to biotech and biopharma companies, government agencies including FDA and NIH, and universities. Clients' samples of hybridoma cell lines or clonal B cells just need to meet 2 requirements: functional antibodies are secreted by the cells; enough RNA remains.
- Save time. One may learn how to clone and sequence antibody genes by studying hybridoma antibody cloning and antibody sequencing protocols that we collected from public literature. Generally speaking, it is more difficult to clone antibody genes from rodents than other species; among isotypes, it is the easiest to clone IgG genes, especially IgG1. A couple of companies sell such kits and PCR primers. However, it takes time to become a highly efficient and experienced professional for so many lab skills and bioinformatics analysis involved in such projects. In addition, there are some samples invaluable but difficult to work. Some service suppliers spend months but could not solve the problem, just wasting clients' samples and time.
- Save money. Since we process samples from multiple clients in the same time, our cost is lower than clients'. Our low cost molecular biology reagents further save clients' cost.

Custom Ilama and alpaca monoclonal antibody development (VHH, sdAb or nanobody) by Ilama or alpaca immunization and phage display screening.

Production of camel, Ilama or alpaca VHH in E.coli or VHH-Fc in HEK 293 cells.

Fast turnaround: 4-6 months once the antigen is available.

PA007187: In Vivo Grade Recombinant Chimeric Human IgG1 kappa Isotype Control Antibody

The in vivo grade recombinant chimeric human IgG1 isotype control antibody was produced in CHO cells.
Applications: an isotype-matched negative control for human IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007188: In Vivo Grade Recombinant Chimeric Human IgG1-D265A kappa Isotype Control Antibody

The in vivo grade recombinant chimeric human IgG1-D265A kappa isotype control mutant was produced in mammalian cells.
Applications: an isotype-matched negative control for human IgG1 D265A kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007189: In Vivo Grade Recombinant Chimeric Human IgG1-N297A kappa Isotype Control Antibody

The in vivo grade recombinant chimeric human IgG1-N297A kappa isotype control mutant was produced in mammalian cells.
Applications: an isotype-matched negative control for human IgG1 N297A kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007190: In Vivo Grade Recombinant Chimeric Human IgG1-L234A L235A P329G (hIgG1 LALAPG) kappa Isotype Control Antibody

The in vivo grade recombinant chimeric human IgG1-L234A L235A P329G (hIgG1 LALAPG, Fc silenced) kappa isotype control mutant was produced in CHO cells.
Applications: an isotype-matched negative control for human IgG1 LALAPG kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007191: In Vivo Grade Recombinant Chimeric Human IgG1-D265A N297A (hIgG1 DANA) kappa Isotype Control Antibody

The in vivo grade recombinant chimeric human IgG1-D265A N297A (hIgG1 DANA) kappa isotype control mutant was produced in mammalian cells.
Applications: an isotype-matched negative control for human IgG1 DANA kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007192: In Vivo Grade Recombinant Chimeric Human IgG1-S267E L328F (hIgG1 SELF) kappa Isotype Control Antibody

The in vivo grade recombinant chimeric human IgG1-S267E L328F (hIgG1 SELF, Fc activated) kappa isotype control mutant was produced in mammalian cells.
Applications: an isotype-matched negative control for human IgG1 SELF kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007193: In vivo Grade Recombinant Chimeric Human IgG2 Isotype Control Antibody

Source: CHO cell-derived.
Applications: an isotype-matched negative control for human IgG2 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007194: In Vivo Grade Recombinant Chimeric Human IgG4-S228P Isotype Control Antibody (Chimeric hIgG4 S228P Isotype Control)

The recombinant chimeric human IgG4-S228P isotype control antibody (chimeric hIgG4 S228P isotype control) was produced in CHO cells.
Applications: an isotype-matched negative control for human IgG4 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007195: In Vivo Grade Recombinant Chimeric Human IgG4-S228P L235E P329G Isotype Control Antibody (Chimeric hIgG4 SPLEPG Isotype Control Mutant)

The recombinant chimeric human IgG4-S228P L235E P329G isotype control antibody (chimeric hIgG4 SPLEPG isotype control mutant, Fc silenced) was produced in mammalian cells.
Applications: an isotype-matched negative control for human IgG4 SPLEPG kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

The most comprehensive hybridoma-based mouse monoclonal antibody production services: custom production of monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins as well as small molecular compounds; for ELISA, Western blot, IHC, and flow cytometry applications.

The lowest custom mouse monoclonal antibody production price: $6000.

Fast turnaround: 24 weeks once the antigen is available.

PA007146: In Vivo Grade Recombinant Armenian Hamster IgG1 Isotype Control Antibody

The in vivo grade recombinant hamster IgG1 isotype control antibody was produced in mammalian cells.
Applications: an isotype-matched negative control for hamster IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin: Less than 1 EU/mg of protein as determined by LAL method.

PA007148: In Vivo Grade Recombinant Syrian Hamster IgG1 Isotype Control Antibody

The in vivo grade recombinant hamster IgG1 isotype control antibody was produced in mammalian cells.
Applications: an isotype-matched negative control for hamster IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin: Less than 1 EU/mg of protein as determined by LAL method.

PA007147: In Vivo Grade Recombinant Armenian Hamster IgG2 Isotype Control Antibody

The in vivo grade recombinant Armenian hamster IgG2 isotype control antibody was produced in mammalian cells.
Applications: an isotype-matched negative control for hamster IgG2 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin: Less than 1 EU/mg of protein as determined by LAL method.

BP005038: Human CD24 Protein with the N-GST Tag

Source: The recombinant Human CD24 Protein, (amino acids Ser27-Gly59) fused with a GST tag at the N-terminus, was produced in E.coli cells.
Activity: Immobilized recombinant Human CD24 protein with the N-GST tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-CD24 Antibody protein with the N-hFc tag with the EC50 of 4.5 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP003511: Human CD24 Protein with the C-His-Avi Tag

Source: The recombinant Human CD24 Protein, (amino acids Ser27-Gly59) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD24 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-CD24 Antibody protein with the C-hFc tag with the EC50 of 67.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005414: Human CD24 Protein with the C-hFc Tag

Source: The recombinant Human CD24 Protein, (amino acids Ser27-Gly59) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD24 protein with the C-hFc tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Anti-CD24 Antibody protein with the C-hFc tag with the EC50 of 0.58 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005415: Human CD24 Protein with the C-mFc Tag

Source: The recombinant Human CD24 Protein, (amino acids Ser27-Gly59) fused with a mFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD24 protein with the C-mFc tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-CD24 Antibody protein with the C-hFc tag with the EC50 of 0.28 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005471: Human CD24 Protein with the C-Llama Fc Tag

Source: The recombinant Human CD24 Protein, (amino acids Ser27-Gly59) fused with a Llama Fc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD24 protein with the C-Llama Fc tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Anti-CD24 Antibody protein with the C-hFc tag with the EC50 of 0.26 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP4091: In Vivo Grade Recombinant Human IgG1 Fc protein (hIgG1 Fc)

The recombinant human IgG1 Fc protein was produced in mammalian cells and purified by protein A.
Purity: >95% by SDS-PAGE under reducing conditions
Endotoxin: Less than 1 EU/mg of protein as determined by LAL method.

Recombinant human IgG1 Fc protein mutants available including hIgG1 Fc C103S; hIgG1 Fc D265A; hIgG1 Fc R214K; hIgG1 Fc R214K/N297A; hIgG1 Fc L234A/L235A; hIgG1 Fc L234F/L235E/P331S; hIgG1 Fc L235A/G237A. Recombinant human IgG1 isotype control antibody and mutants are available. Biotinylated hIgG1 Fc, and hIgG1 Fc with Avi-, His-, and Flag-tags are also available.