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Conditions of optimal recombinant Human IL-2 R alpha/CD25 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
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BP005391: Human IL-2 R alpha/CD25 Protein with the C-His-Avi Tag
Source: The recombinant Human IL-2 R alpha/CD25 Protein, (amino acids Glu22-Cys213) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human IL-2 R alpha/CD25 protein with the His-Avi tag at the C-terminus has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human IL-2 R alpha protein with the C-His-Avi tag at 0.2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-IL-2 R alpha Antibody protein with the C-hFc tag with the EC50 of 9.6 ng/ml determined by ELISA (QC Test). The affinity constant of 15.2 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human IL-2 R alpha/CD25 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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