PA005172-ANT-181: Anthrax LF Polyclonal Antibody

Rabbit Polyclonal Antibody;
Applications: WB;
Isotype: Rabbit Ig.

PA005173-ANT-182: Anthrax PA Polyclonal Antibody

Rabbit Polyclonal Antibody;
Applications: WB;
Isotype: Rabbit Ig.

PA007166.m2a: In Vivo Grade Recombinant Anti-Mouse CD8a Mouse IgG2a Kappa Monoclonal Antibody

The in vivo grade recombinant anti-mouse CD8a mouse IgG2a monoclonal antibody (clone of YTS 169.4) was produced in mammalian cells.
Applications: Western blot, immunohistochemistry (IHC), Flow Cytometry (FC), and in vivo CD8+ T cell depletion.

PA007166.r2b: In Vivo Grade Recombinant Anti-Mouse CD8a Rat IgG2b Kappa Monoclonal Antibody

The in vivo grade recombinant anti-mouse CD8a rat IgG2b monoclonal antibody (clone of YTS 169.4) was produced in mammalian cells.
Applications: Western blot, immunohistochemistry (IHC), Flow Cytometry (FC), and in vivo CD8+ T cell depletion.

PA007167.m2a: In Vivo Grade Recombinant Anti-Mouse CD8a Mouse IgG2a Kappa Monoclonal Antibody

The in vivo grade recombinant anti-mouse CD8a mouse IgG2a monoclonal antibody (clone of 2.43) was produced in mammalian cells.
Applications: Western blot, immunohistochemistry (IHC), Flow Cytometry (FC), and in vivo CD8+ T cell depletion.

PA007167.r2b: In Vivo Grade Recombinant Anti-Mouse CD8a Rat IgG2b Kappa Monoclonal Antibody

The in vivo grade recombinant anti-mouse CD8a rat IgG2b monoclonal antibody (clone of 2.43) was produced in mammalian cells.
Applications: Western blot, immunohistochemistry (IHC), Flow Cytometry (FC), and in vivo CD8+ T cell depletion.

PA007168.m2a: In Vivo Grade Recombinant Anti-Mouse OX40 Mouse IgG2a Kappa Monoclonal Antibody

The in vivo grade recombinant anti-mouse OX40 mouse IgG2a monoclonal antibody (clone of OX86) was produced in mammalian cells.
Applications: ELISA, Western blot (WB), immunofluorescence (IF), Flow Cytometry (FC), and various in vitro and in vivo functional assays.

PA007168.r1: In Vivo Grade Recombinant Anti-Mouse CD8a Rat IgG1 Kappa Monoclonal Antibody

The in vivo grade recombinant anti-mouse OX40 rat IgG1 monoclonal antibody (clone of OX86) was produced in mammalian cells.
Applications: ELISA, Western blot (WB), immunofluorescence (IF), Flow Cytometry (FC), and various in vitro and in vivo functional assays.

PA007163.m2cLA: In Vivo Grade Recombinant Anti-mouse PD-1 Mouse IgG2c-L234A L235A P329G (LALAPG) Kappa Monoclonal Antibody (Clone 29F.1A12.1)

The in vivo grade recombinant anti-mouse PD-1 monoclonal antibody (mouse IgG2c-LALAPG kappa) was produced in mammalian cells.
Applications: western blot (WB), immunohistochemistry (IHC), Flow Cytometry (FC), and various in vitro and in vivo functional assays.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007163.r2a: In Vivo Grade Recombinant Anti-mouse PD-1 Rat IgG2a Kappa Monoclonal Antibody (Clone 29F.1A12.1)

The in vivo grade recombinant anti-mouse PD-1 monoclonal antibody (rat IgG2a kappa) was produced in mammalian cells.
Applications: western blot (WB), immunohistochemistry (IHC), Flow Cytometry (FC), and various in vitro and in vivo functional assays.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Fast turnaround: 3-6 months with our proprietary optimization algorithm.

Antibody affinity can be improved by about 10 folds after each round of antibody affinity maturation.

100000 cells per hybridoma clone are sufficient for cloning and sequencing the monoclonal antibody variable regions (both VH and VL) and full length. Welcome to challenge us with smaller number of clonal B cells or non-viable cells for hybridoma rescue.

- Reliable results. With optimized workflow and PCR primers, we have successfully cloned and sequenced >3000 antibodies from various species in the past 10 years. Syd Labs has become a reliable CRO supplier of antibody sequencing to biotech and biopharma companies, government agencies including FDA and NIH, and universities. Clients' samples of hybridoma cell lines or clonal B cells just need to meet 2 requirements: functional antibodies are secreted by the cells; enough RNA remains.
- Save time. One may learn how to clone and sequence antibody genes by studying hybridoma antibody cloning and antibody sequencing protocols that we collected from public literature. Generally speaking, it is more difficult to clone antibody genes from rodents than other species; among isotypes, it is the easiest to clone IgG genes, especially IgG1. A couple of companies sell such kits and PCR primers. However, it takes time to become a highly efficient and experienced professional for so many lab skills and bioinformatics analysis involved in such projects. In addition, there are some samples invaluable but difficult to work. Some service suppliers spend months but could not solve the problem, just wasting clients' samples and time.
- Save money. Since we process samples from multiple clients in the same time, our cost is lower than clients'. Our low cost molecular biology reagents further save clients' cost.

Kinetic analysis is used to determine the affinity of an antibody to its soluble antigen using surface plasmon resonance (SPR) or biolayer interferometry (BLI), which can measure kon, koff, and kD. Association and dissociation are measured on the Biacore T100 or the ForteBio Octet QKe using various concentrations of the antigen and the kD is calculated using an appropriate curve-fitting model.

Fast turnaround time: 2-3 days per antibody-antigen pair, but may require more time depending on the complexity of analysis.

BP001218-C207: Recombinant Human Annexin A6 (ANXA6) Protein

Source: E. coli-derived.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug).