Anti-Mouse CD8a Monoclonal Antibodies

Background

The rat anti-mouse CD8a monoclonal antibody YTS 169.4  (rat IgG2b kappa) reacts with the mouse CD8a protein (T-cell surface glycoprotein CD8 alpha chain) encoded by the mouse CD8A gene that encodes the CD8a chain of the dimeric CD8 protein. The mouse CD8 protein is primarily responsible for cell-mediated immune defense and T-cell development. CD8A has been widely reported as a potential prognosis and diagnostic marker for several diseases, such as inflammatory disorders and tumors. It was shown that the anti-mouse CD8a rat monoclonal antibody (clone number: YTS 169.4) can be used for depleting activity in vivo.

Our recombinant YTS 169.4 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse CD8a monoclonal antibody (hybridoma clone name or number: YTS 169.4).

PA007166.m2a: In Vivo Grade Recombinant Anti-Mouse CD8a Mouse IgG2a Kappa Monoclonal Antibody

The in vivo grade recombinant anti-mouse CD8a mouse IgG2a monoclonal antibody was produced in mammalian cells.
Specificity: CD8a.
Clone: YTS 169.4.
Isotype: mouse IgG2a, kappa.
Applications: Western blot, immunohistochemistry (IHC), Flow Cytometry (FC), and in vivo CD8+ T cell depletion.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007166.r2b: In Vivo Grade Recombinant Anti-Mouse CD8a Rat IgG2b Kappa Monoclonal Antibody

The in vivo grade recombinant anti-mouse CD8a rat IgG2b monoclonal antibody was produced in mammalian cells.
Specificity: CD8a.
Clone: YTS 169.4.
Isotype: rat IgG2b, kappa.
Applications: Western blot, immunohistochemistry (IHC), Flow Cytometry (FC), and in vivo CD8+ T cell depletion.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant anti-mouse CD8a antibodies (clone of YTS 169.4) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

References of anti-mouse CD8a antibody (YTS 169.4)

Exhausted effector memory CD8 T cells expand in chronic EAU.
Boldison J, et al. Invest Ophthalmol Vis Sci. 2013 Oct 3;54(10):6446-54. doi: 10.1167/iovs.13-12429. PMID: 24091325
For in vivo depletion, anti-CD8 (YTS 169.4, 100 ?g) was administered intraperitoneally on days -3 and 0 relative to immunization. Flow cytometry was used to quantify retinal infiltration and confirm CD8+ T cell depletion. Chronic experimental autoimmune uveoretinitis (EAU) was induced in B10.RIII mice by immunization with IRBP peptide. Effector memory CD8 T cells were found to expand in the chronic phase of EAU. Depletion of CD8+ T cells reduced retinal inflammation but did not eliminate disease progression.
Tags: anti-mouse CD8a; in vivo study of YTS 169.4 antibody

Site-restricted persistent cytomegalovirus infection after selective long-term depletion of CD4+ T lymphocytes.
Jonji? S, et al. J Exp Med. 1989 Apr 1;169(4):1199-212. doi: 10.1084/jem.169.4.1199. PMID: 2703885
The mAbs used for depletion were anti-L3T4 (clone YTS 191.1) and anti-Lyt-2 (clone YTS 169.4) at a dose of 1 mg per mouse, administered intraperitoneally. Selective depletion of CD4+ T lymphocytes was performed to study persistent cytomegalovirus infection. Viral titers were measured in salivary glands and lungs of infected mice. CD4+ T cell depletion led to site-restricted viral persistence in the salivary glands. The study demonstrated the critical role of CD4+ T cells in controlling cytomegalovirus infection.
Tags: anti-mouse CD8a YTS 169.4 antibody in vivo; anti-mouse CD8a YTS 169.4 antibody in animal model

In vivo depletion of murine CD8 positive T cells impairs survival during infection with a highly virulent strain of Cryptococcus neoformans.
Mody CH, et al. Mycopathologia. 1994 Feb;125(2):77-84. doi: 10.1007/BF01103969. PMID: 8057664
Mice were injected intraperitoneally with 100 ?g of anti-CD8 monoclonal antibody (YTS 169.4) to deplete CD8+ T cells in vivo. Survival was monitored in mice infected with a highly virulent strain of Cryptococcus neoformans. Lung fungal burden was assessed by quantitative culture. Flow cytometry confirmed the depletion of CD8+ T cells in peripheral blood. CD8+ T cell depletion significantly reduced survival compared to control mice.
Tags: anti-mouse CD8a YTS 169.4 in cancer research; anti-mouse CD8a YTS 169.4 in mouse tumor model

Therapy with monoclonal antibodies by elimination of T-cell subsets in vivo.
Cobbold SP, et al. Nature. 1984 Dec 6-12;312(5994):548-51. doi: 10.1038/312548a0. PMID: 6392460
The rat anti-mouse Lyt-2 monoclonal antibody (YTS 169.4) was used to eliminate T-cell subsets in vivo in ATX mice. Monoclonal antibodies were administered intraperitoneally to deplete specific T-cell populations. The efficacy of T-cell depletion was confirmed by flow cytometry of spleen cells. Skin graft survival was prolonged in mice treated with anti-T-cell antibodies. The study demonstrated the therapeutic potential of monoclonal antibodies in immunosuppression.
Tags: anti-mouse CD8a YTS 169.4 mAb in mouse tumor model; function of anti-mouse CD8a YTS 169.4 mAb

CD4 monoclonal antibody pairs for immunosuppression and tolerance induction.
Qin S, et al. Eur J Immunol. 1987 Aug;17(8):1159-65. doi: 10.1002/eji.1830170813. PMID: 3303040
Anti-CD8 (YTS 169.4) and anti-CD4 (YTS 191.1) antibodies were administered intraperitoneally to achieve immunosuppression in vivo. The combination of monoclonal antibodies was tested for its ability to induce tolerance to skin grafts. Flow cytometry was used to monitor T-cell depletion in peripheral blood and lymphoid organs. Long-term graft survival was achieved in mice treated with both antibodies. The study highlighted the synergistic effect of anti-CD4 and anti-CD8 antibodies in tolerance induction.
Tags: anti-mouse CD8a YTS 169.4 antibody of low endotoxin; anti-mouse CD8a (YTS 169.4)

For more references about anti-mouse CD8a antibody (YTS 169.4), please contact our scientific support team with message@sydlabs.com.

In Vivo Grade Recombinant Anti-Mouse CD8a Monoclonal Antibodies (Clone YTS 169.4):
In Vivo Grade Recombinant Anti-Mouse CD8a Mouse IgG2a Monoclonal Antibody
In Vivo Grade Recombinant Anti-Mouse CD8a Mouse Fab Fragment
In Vivo Grade Recombinant Anti-Mouse CD8a Rat IgG2b Monoclonal Antibody

Syd Labs provides the following recombinant anti-human CD8a monoclonal antibodies:
In vivo grade recombinant anti-human CD8a antibodies (clone OKT8)
Recombinant anti-human CD8a antibodies (clone OKT8) for flow cytometry

Syd Labs provides the following recombinant anti-mouse CD8a monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8a antibodies (clone 2.43)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 169.4)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 105.18)

Syd Labs provides the following recombinant anti-mouse CD8b monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8b antibodies (clone YTS 156.7)