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Recombinant anti-mouse CD8a monoclonal antibodies from the variable region sequences of the rat anti-mouse CD8a monoclonal antibody (clone number: 2.43) are produced from mammalian cells and good for in vitro and in vivo studies.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The rat anti-mouse CD8a monoclonal antibody 2.43 (rat IgG2b kappa) reacts with the mouse CD8a protein (T-cell surface glycoprotein CD8 alpha chain) encoded by the mouse CD8A gene that encodes the CD8a chain of the dimeric CD8 protein. The mouse CD8 protein is primarily responsible for cell-mediated immune defense and T-cell development. CD8A has been widely reported as a potential prognosis and diagnostic marker for several diseases, such as inflammatory disorders and tumors. It was shown that the anti-mouse CD8a rat monoclonal antibody (clone number: 2.43) can be used for depleting activity in vivo.
Our recombinant 2.43 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse CD8a monoclonal antibody (hybridoma clone name or number: 2.43).
The in vivo grade recombinant anti-mouse CD8a mouse IgG2a monoclonal antibody was produced in mammalian cells.
Specificity: CD8a.
Clone: 2.43.
Isotype: mouse IgG2a, kappa.
Applications: Western blot, immunohistochemistry (IHC), Flow Cytometry (FC), and in vivo CD8+ T cell depletion.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
The in vivo grade recombinant anti-mouse CD8a rat IgG2b monoclonal antibody was produced in mammalian cells.
Specificity: CD8a.
Clone: 2.43.
Isotype: rat IgG2b, kappa.
Applications: Western blot, immunohistochemistry (IHC), Flow Cytometry (FC), and in vivo CD8+ T cell depletion.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant anti-mouse CD8a antibodies (clone of 2.43) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.
1、Macrophage Migration Inhibitory Factor protects cancer cells from immunogenic cell death and impairs anti-tumor immune responses
Balogh KN, et al. PLoS One. 2018 Jun 4;13(6):e0197702. doi: 10.1371/journal.pone.0197702. PMID: 29864117.
Beginning two days before 4T1 cell tumor implantation, mice were treated with intraperitoneal injection of an initial dose of 200ug/mouse of anti-CD4 (clone GK1.5) and anti-CD8 (clone 2.43) antibodies in PBS, followed by similar dosing with 100ug/mouse every 4 days throughout the course of tumor growth. Tumors were excised on day 20 and weighed.
Tags: anti-mouse CD8a antibody; 2.43 antibody
2、VEGF-A modulates expression of inhibitory checkpoints on CD8+ T cells in tumors
Voron T, et al. J Exp Med. 2015 Feb 9;212(2):139-48. doi: 10.1084/jem.20140559. Epub 2015 Jan 19. PMID: 25601652.
After treatments, tumors were dissociated using Gentle Macs dissociator (Miltenyi Biotec). For CD8+ T cell depletion studies, anti-CD8 antibody (clone 2.43) or isotype control antibody (rat IgG2a) was administered at 100 µg i.p. to CT26-tumor bearing mice the day before starting anti-VEGFA treatment. CD8 depletion was checked by flow cytometry.
Tags: anti-mouse CD8a antibody; 2.43 antibody
3、Co-inhibitory Molecule B7 Superfamily Member 1 Expressed by Tumor-Infiltrating Myeloid Cells Induces Dysfunction of Anti-tumor CD8+ T Cells
Li J, et al. 2018 Apr 17;48(4):773-786.e5. doi: 10.1016/j.immuni.2018.03.018. Epub 2018 Apr 3. PMID: 29625896.
For A20 model, 100 μg control or anti-B7S1 antibody was injected intraperitoneally on Day 5, 7, 9, 11, 13, 15, 17, 19, 21 and 23. To test the therapeutic effect of combinational blockade of B7S1 and PD-1, control antibodies, 100 μg anti-B7S1, 75 μg anti-PD-1 (J43) or 100 μg anti-B7S1+75 μg anti-PD-1 were injected i.p. every 3 days starting from Day 9. To deplete CD4+ or CD8+ T cells, 100 μg of anti-CD4 (GK 1.5) or anti-CD8 (2.43) was injected i.p. on Day −1, 2, 7, 12 and 17. To deplete NK cells, 20 μg of anti-asialo GM1 was injected i.v. on Day −1, 4, 9, 14 and 19. The depletion efficiency was up to 85%.
Tags: anti-mouse CD8a 2.43 antibody; anti-mouse CD8a 2.43 in vivo
For more references about anti-mouse CD8a antibody (2.43) please contact our scientific support team with message@sydlabs.com.
In Vivo Grade Recombinant Anti-Mouse CD8a Monoclonal Antibodies (Clone 2.43):
In Vivo Grade Recombinant Anti-Mouse CD8a Mouse IgG2a Monoclonal Antibody
In Vivo Grade Recombinant Anti-Mouse CD8a Mouse Fab Fragment
In Vivo Grade Recombinant Anti-Mouse CD8a Rat IgG2b Monoclonal Antibody
Syd Labs provides the following recombinant anti-human CD8a monoclonal antibodies:
In vivo grade recombinant anti-human CD8a antibodies (clone OKT8)
Recombinant anti-human CD8a antibodies (clone OKT8) for flow cytometry
Syd Labs provides the following recombinant anti-mouse CD8a monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8a antibodies (clone 2.43)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 169.4)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 105.18)
Syd Labs provides the following recombinant anti-mouse CD8b monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8b antibodies (clone YTS 156.7)
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