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Recombinant rat IgG2b isotype controls are available. Recombinant anti-mouse CD8a monoclonal antibodies from the variable region sequences of the rat anti-mouse CD8a monoclonal antibody (clone number: 2.43) are produced from mammalian cells and good for in vitro and in vivo studies.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The rat anti-mouse CD8a monoclonal antibody clone 2.43 (also widely referred to as anti-Lyt-2 antibody) specifically reacts with the mouse CD8a protein—a T-cell surface glycoprotein encoded by the CD8A gene. As a critical component of the dimeric CD8 protein, CD8a is essential for cell-mediated immune defense and T-cell development. Beyond its physiological role, CD8A is recognized as a vital diagnostic and prognostic marker in inflammatory disorders and various tumor models.
Our in vivo grade recombinant anti-mouse CD8a monoclonal antibody (clone 2.43) is engineered to provide superior performance over traditional hybridoma-derived products. By utilizing a low endotoxin production platform, we ensure that each batch meets stringent requirements for in vivo CD8+ T cell depletion assays. Our recombinant 2.43 antibodies possess the complete amino acid sequences of the original hybridoma clone, guaranteeing high-fidelity binding, exceptional batch-to-batch consistency, and the reliability required for pristine, publication-ready research datasets.
The in vivo grade recombinant anti-mouse CD8a rat IgG2b monoclonal antibody was produced in mammalian cells.
Specificity: CD8a.
Clone: 2.43.
Isotype: rat IgG2b, kappa.
Applications: Western blot, immunohistochemistry (IHC), Flow Cytometry (FC), and in vivo CD8+ T cell depletion.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
RRID: AB_3697157
The in vivo grade recombinant anti-mouse CD8a mouse IgG2a monoclonal antibody was produced in mammalian cells.
Specificity: CD8a.
Clone: 2.43.
Isotype: mouse IgG2a, kappa.
Applications: Western blot, immunohistochemistry (IHC), Flow Cytometry (FC), and in vivo CD8+ T cell depletion.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant anti-mouse CD8a antibodies (clone of 2.43) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
12 months from date of receipt, -20°C to -70°C as supplied.
Yes, clone 2.43 is the gold-standard antibody for in vivo CD8+ T cell depletion in mouse models. Syd Labs provides a recombinant, sequence-defined, low-endotoxin version of clone 2.43, engineered to ensure high depletion efficiency (>90%) with minimal non-specific immune activation.
For an optimal mouse CD8+ T cell depletion protocol, typical dosage ranges from 100 µg to 500 µg per mouse per intraperitoneal (i.p.) injection, depending on your specific model (e.g., tumor suppression vs. viral infection). Consistency in injection frequency (e.g., every 3-4 days) is key to maintaining sustained depletion levels. Please refer to our "References" section for validated protocols utilized in published experimental models.
Yes, incorporating an isotype control is critical to distinguish target-specific depletion effects from Fc-receptor-mediated background noise. We strongly recommend using our highly purified rat IgG2b or mouse IgG2a isotype controls, precisely matching the isotype of your selected 2.43 product, to ensure your experimental readouts are statistically robust and publication-ready.
Traditional hybridoma lines are highly susceptible to genetic drift, chromosomal loss, and inherent batch-to-batch variation. Syd Labs' recombinant anti-mouse CD8a 2.43 is produced in mammalian cells, ensuring absolute sequence consistency. This advanced platform guarantees ultra-low endotoxin levels (<1 EU/mg), eliminating the toxicity often seen with traditional hybridoma formats and making it the superior choice for reproducible research.
The most widely accepted standard approach is to perform Flow Cytometry (FC) analysis on peripheral blood, spleen, or lymph node cell suspensions 24-48 hours after the initial injection. Important: You must use a different non-competing anti-CD8a clone (such as clone 53-6.7) for your fluorescent staining to accurately quantify the remaining T cells without competitive interference from the depleting 2.43 antibody.
When publishing your research using this product, please cite our antibody as: "Recombinant Anti-Mouse CD8a Monoclonal Antibody (Syd Labs, Cat# PA007167.r2b; RRID: AB_3697157)." (Note: If using the mouse IgG2a variant, please update the Cat# to PA007167.m2a).
In Vivo Grade Recombinant Anti-Mouse CD8a Monoclonal Antibodies (Clone 2.43):
In Vivo Grade Recombinant Anti-Mouse CD8a Mouse IgG2a Monoclonal Antibody
In Vivo Grade Recombinant Anti-Mouse CD8a Mouse Fab Fragment
In Vivo Grade Recombinant Anti-Mouse CD8a Rat IgG2b Monoclonal Antibody
Syd Labs provides the following recombinant anti-human CD8a monoclonal antibodies:
In vivo grade recombinant anti-human CD8a antibodies (clone OKT8)
Recombinant anti-human CD8a antibodies (clone OKT8) for flow cytometry
Syd Labs provides the following recombinant anti-mouse CD8a monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8a antibodies (clone 2.43)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 169.4)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 105.18)
Syd Labs provides the following recombinant anti-mouse CD8b monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8b antibodies (clone YTS 156.7)
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