Anti-Mouse CD8a Monoclonal Antibodies for Flow Cytometry

PA007498.r2b: Recombinant Anti-mouse CD8a Monoclonal Antibody (Clone: 2.43), Rat IgG2b Kappa

Recombinant Rat IgG2b Monoclonal Antibody. 
Clone: 2.43.
Isotype:  Rat IgG2b Kappa.
Source: The anti-mouse CD8a monoclonal antibody (clone: 2.43) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 2.43 antibody specifically binds to mouse CD8a protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007498.h1Fs: Recombinant Anti-mouse CD8a Monoclonal Antibody (Clone: 2.43), Human IgG1 Fc Silent Kappa

Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: 2.43.
Isotype: Human IgG1 kappa.
Source: The anti-mouse CD8a monoclonal antibody (clone: 2.43) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 2.43 antibody specifically binds to mouse CD8a protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The 2.43 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.

Background

The rat anti-mouse CD8a monoclonal antibody 2.43 (rat IgG2b kappa) reacts with the mouse CD8a protein (T-cell surface glycoprotein CD8 alpha chain) encoded by the mouse CD8A gene that encodes the CD8a chain of the dimeric CD8 protein. The mouse CD8 protein is primarily responsible for cell-mediated immune defense and T-cell development. CD8A has been widely reported as a potential prognosis and diagnostic marker for several diseases, such as inflammatory disorders and tumors. It was shown that the anti-mouse CD8a rat monoclonal antibody (clone number: 2.43) can be used for depleting activity in vivo.
Our recombinant 2.43 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse CD8a monoclonal antibody (hybridoma clone name or number: 2.43).

References of anti-mouse CD8a antibody (2.43)

T cell mediated immunity after combination therapy with intralesional PV-10 and blockade of the PD-1/PD-L1 pathway in a murine melanoma model
Liu H, et al. (2018). PLoS One. 2018 Apr 16;13(4):e0196033. doi: 10.1371/journal.pone.0196033. PMID: 29668690
To deplete CD8+ T cells, mice were injected intraperitoneally with 100 μg of anti-CD8 (2.43 antibody) on days -3, 0, and every 3–4 days thereafter. ... Tumor growth was measured every 2–3 days using calipers, and tumor volume was calculated as (length × width²)/2. ... Flow cytometry was performed to assess PD-1 expression on tumor-infiltrating lymphocytes. ... Combination therapy with PV-10 and anti-PD-1 significantly reduced tumor burden compared to monotherapy. ... Depletion of CD8+ T cells abrogated the therapeutic effect of the combination treatment.
Tags: function of 2.43 antibody; 2.43 antibody for flow cytometry

CD4 T cells mediate cardiac xenograft rejection via host MHC Class II
Plenter RJ, et al. (2012). J Heart Lung Transplant. 2012 Jul;31(7):781-9. doi: 10.1016/j.healun.2012.03.018. PMID: 22560623
To deplete CD4 T cells, 20 mg/kg of anti-CD4 antibody (clone GK1.5) was injected intraperitoneally into mice, while CD8 depletion was accomplished with 20 mg/kg of 2.43 antibody. ... Cardiac xenografts were transplanted into the abdomen of recipient mice and monitored for rejection. ... Flow cytometry confirmed the efficacy of T cell depletion in peripheral blood. ... Rejection was assessed by palpation, with cessation of graft beating indicating rejection. ... Host MHC Class II expression was critical for CD4 T cell-mediated xenograft rejection.
Tags: 2.43 antibody mouse dose range; 2.43 mAb for cancer research

Selective Targeting of Antitumor Immune Responses with Engineered Live-Attenuated Listeria monocytogenes
Yoshimura K, et al. (2006). Cancer Res. 2006 Jan 15;66(2):1096-104. doi: 10.1158/0008-5472.CAN-05-2302. PMID: 16424011
For depletion studies, 0.5 mg of anti-CD8 (2.43 antibody) was injected intraperitoneally on days -3 and -1 before tumor challenge. ... Listeria monocytogenes expressing tumor antigens was administered to induce antitumor immunity. ... Flow cytometry was used to confirm CD8+ T cell depletion in liver and spleen. ... Tumor growth was significantly reduced in mice treated with the engineered Listeria. ... The therapeutic effect was dependent on CD8+ T cell responses.
Tags: 2.43 mAb for mouse tumor model; clone 2.43 of anti-mouse CD8a

NK and CD8+ T cell-mediated eradication of poorly immunogenic B16-F10 melanoma by the combined action of IL-12 gene therapy and 4-1BB costimulation
Xu D, et al. (2004). Int J Cancer. 2004 Feb 20;109(4):499-506. doi: 10.1002/ijc.11696. PMID: 14627990
To identify the cell types responsible for antitumor activity, leukocytes were depleted in vivo every other day for 3 times with 2.43 antibody (rat anti-mouse CD8). ... IL-12 gene therapy was delivered intratumorally to B16-F10 melanoma-bearing mice. ... 4-1BB costimulation was provided via agonistic anti-4-1BB antibody injections. ... Flow cytometry confirmed the depletion of CD8+ T cells in treated mice. ... Tumor regression was significantly enhanced in the combined treatment group.
Tags: anti-mouse CD8a 2.43 of low endotoxin; function of anti-mouse CD8a 2.43

Induction of CD4+ T-Cell-Independent Immunoglobulin Responses by Inactivated Influenza Virus
Sha Z, et al. (2000). J Virol. 2000 Jun;74(11):4999-5005. doi: 10.1128/JVI.74.11.4999-5005.2000. PMID: 10799866
To deplete CD8+ T cells, mice were injected intraperitoneally with 0.5 mg of 2.43 antibody on days -3 and -1. ... Inactivated influenza virus was administered to induce immunoglobulin responses. ... Flow cytometry was used to confirm CD8+ T cell depletion in spleen and lymph nodes. ... IgG responses were maintained in the absence of CD4+ T cells, indicating T-cell-independent mechanisms. ... The study demonstrated the role of B cells in antiviral immunity.
Tags: anti-mouse CD8a 2.43 antibody for flow cytometry; anti-mouse CD8a 2.43 mAb

For more references about anti-mouse CD8a antibody (2.43), please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following recombinant anti-human CD8a monoclonal antibodies:
In vivo grade recombinant anti-human CD8a antibodies (clone OKT8)
Recombinant anti-human CD8a antibodies (clone OKT8) for flow cytometry

Syd Labs provides the following recombinant anti-mouse CD8a monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8a antibodies (clone 2.43)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 169.4)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 105.18)

Syd Labs provides the following recombinant anti-mouse CD8b monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8b antibodies (clone YTS 156.7)