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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant rat IgG2b isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2b Monoclonal Antibody.
Clone: 2.43.
Isotype: Rat IgG2b Kappa.
Source: The anti-mouse CD8a monoclonal antibody (clone: 2.43) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 2.43 antibody specifically binds to mouse CD8a protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: 2.43.
Isotype: Human IgG1 kappa.
Source: The anti-mouse CD8a monoclonal antibody (clone: 2.43) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 2.43 antibody specifically binds to mouse CD8a protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: 2.43.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated anti-mouse CD8a monoclonal antibody (clone: 2.43) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant 2.43 antibody specifically binds to mouse CD8a protein.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The 2.43 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Background
The rat anti-mouse CD8a monoclonal antibody 2.43 (rat IgG2b kappa) reacts with the mouse CD8a protein (T-cell surface glycoprotein CD8 alpha chain) encoded by the mouse CD8A gene that encodes the CD8a chain of the dimeric CD8 protein. The mouse CD8 protein is primarily responsible for cell-mediated immune defense and T-cell development. CD8A has been widely reported as a potential prognosis and diagnostic marker for several diseases, such as inflammatory disorders and tumors. It was shown that the anti-mouse CD8a rat monoclonal antibody (clone number: 2.43) can be used for depleting activity in vivo.
Our recombinant 2.43 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse CD8a monoclonal antibody (hybridoma clone name or number: 2.43).
1、Macrophage Migration Inhibitory Factor protects cancer cells from immunogenic cell death and impairs anti-tumor immune responses
Balogh, K. N., et al. (2018). PLoS One. doi: 10.1371/journal.pone.0197702. PMID: 29864117
Beginning two days before 4T1 cell tumor implantation, mice were treated with intraperitoneal injection of an initial dose of 200ug/mouse of anti-CD4 (clone GK1.5, BioXCell) and anti-CD8 (clone 2.43, BioXCell) antibodies in PBS, followed by similar dosing with 100ug/mouse every 4 days throughout the course of tumor growth...
Tags: anti-mouse CD8a antibody; 2.43 antibody
2、Co-inhibitory Molecule B7 Superfamily Member 1 Expressed by Tumor-Infiltrating Myeloid Cells Induces Dysfunction of Anti-tumor CD8+ T Cells
Li, J., et al. (2018). Immunity. doi: 10.1016/j.immuni.2018.03.018. PMID: 29625896
Co-inhibitory Molecule B7 Superfamily Member 1 Expressed by Tumor-Infiltrating Myeloid Cells Induces Dysfunction of Anti-tumor CD8+ T Cells To deplete CD4+ or CD8+ T cells, 100 μg of anti-CD4 (GK 1.5, Bioxcell) or anti-CD8 (2.43, Bioxcell) was injected i.p. on Day −1, 2, 7, 12 and 17...
Tags: anti-mouse CD8a 2.43 antibody; anti-mouse CD8a 2.43 in vivo
3、Eradication of large established tumors in mice by combination immunotherapy that engages innate and adaptive immune responses
Moynihan, K. D., et al. (2016). Nat Med. doi: 10.1038/nm.4200. PMID: 27775706
For T cell depletion, 200 μg of anti-CD8α (clone 2.43), anti-CD4 (clone GK1.5), or anti-NK1.1 (clone PK136) (all from BioXCell) were administered i.p. every 3–4 d starting 1 d before treatment...
Tags: anti-mouse CD8a 2.43 antibody in animal model; anti-mouse CD8a 2.43 mAb in animal model
For more references about anti-mouse CD8a antibody for Flow Cytometry (2.43) please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-mouse CD8 monoclonal antibodies:
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: 2.43)
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: YTS 169.4)
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: YTS 105.18)
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: 53-6.72)
Recombinant Anti-mouse CD8b monoclonal antibody (Clone: YTS 156.7)
Recombinant Anti-mouse CD8 (Lyt 2.1) monoclonal antibody (Clone: 116-13.1)
Syd Labs provides the following in vivo grade recombinant anti-human CD8 monoclonal antibodies:
Recombinant Anti-human CD8a monoclonal antibody (Clone: OKT8)
Syd Labs provides the following recombinant anti-mouse CD8 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: 2.43) for flow cytometry
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: YTS 169.4) for flow cytometry
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: YTS 105.18) for flow cytometry
Recombinant Anti-mouse CD8b monoclonal antibody (Clone: YTS 156.7) for flow cytometry
Syd Labs provides the following recombinant anti-human CD8 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD8a monoclonal antibody (Clone: OKT8) for flow cytometry
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