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Home > Antibodies > Anti-Mouse Antibodies for Flow Cytometry > Anti-Mouse CD8b Monoclonal Antibodies

Anti-Mouse CD8b Monoclonal Antibodies

CD8 beta, Lyt-3, Ly-3, T-cell surface glycoprotein CD8 beta chain, CD_antigen CD8b

Catalog No. Product Name Size List Price (US$) Quantity
PA007382.r2b In vivo Grade Recombinant Anti-mouse CD8b Monoclonal Antibody, Rat IgG2b Kappa (Clone: YTS 156.7) 1 mg 150.00
PA007382.r2b In vivo Grade Recombinant Anti-mouse CD8b Monoclonal Antibody, Rat IgG2b Kappa (Clone: YTS 156.7) 5 mg 350.00
PA007382.r2b In vivo Grade Recombinant Anti-mouse CD8b Monoclonal Antibody, Rat IgG2b Kappa (Clone: YTS 156.7) 25 mg 900.00
Description

Background of Anti-mouse CD8b Monoclonal Antibody (Clone: YTS 156.7)

The rat anti-mouse CD8b monoclonal antibody YTS 156.7 (rat IgG2b kappa) reacts with the mouse CD8b protein (T-cell surface glycoprotein CD8 beta chain, Lyt-3 or Ly-3) encoded by the mouse cd8b gene that encodes the CD8b chain of the dimeric CD8 protein. The mouse CD8 protein is primarily responsible for cell-mediated immune defense and T-cell development.

Our recombinant YTS 156.7 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse CD8b monoclonal antibody (hybridoma clone name or number: YTS 156.7).

PA007382.r2b: In vivo Grade Recombinant Anti-mouse CD8b Monoclonal Antibody, Rat IgG2b Kappa (Clone: YTS 156.7)

The in vivo grade recombinant anti-mouse CD8b rat IgG2b monoclonal antibody was produced in mammalian cells. 
Clone: YTS 156.7.
Isotype: Rat IgG2b kappa.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 156.7) specifically binds to mouse CD8b.
Applications: Western blot, immunohistochemistry (IHC), Flow Cytometry (FC), and in vivo CD8+ T cell depletion. 
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant anti-mouse CD8b antibodies (clone of YTS 156.7) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Recombinant Anti-Mouse CD8b Monoclonal Antibody (Clone: YTS 156.7)

Srinivasan, et al. Front Immunol. 2024 Sep 3;15:1412513. doi: 10.3389/fimmu.2024.1412513. PMID: 39253084; PMCID: PMC11381289
Expressed on the surface of CD8+ T cells, the CD8 co-receptor is a key component that contributes to antigen recognition, immune cell maturation, and immune cell signaling. ... This review outlines the unique structure and function of different CD8 domains in the context of the distinct properties of CD8αα homodimers and CD8αβ heterodimers. ... Additionally, the applications of anti-CD8 monoclonal antibodies targeting CD8 are summarized to highlight translational research for targeted immunotherapy.
(Tags: anti-mouse CD8b YTS 156.7 mAb; CD8 beta monoclonal antibody)
Van Der Kraak, et al. Mucosal Immunol. 2021 Sep;14(5):1100-1112. doi: 10.1038/s41385-021-00419-1. PMID: 34103660; PMCID: PMC8562907
Major Histocompatibility Complex Class II (MHCII) is dynamically expressed on intestinal epithelial cells throughout the intestine, but its regulation remains poorly understood. ... We observed that spontaneous upregulation of epithelial MHCII correlated with serum Interleukin (IL)-18 and was driven by an intestinal IL-18-IFNγ axis. ... This process activated or expanded type 1 lymphocytes, suggesting a crucial link between intestinal dysbiosis, systemic inflammation, and epithelial antigen presentation.
(Tags: anti-mouse CD8b T cell depletion; CD8b antibody mucosal immunity)
Poh, et al. Front Immunol. 2020 Mar 17;11:400. doi: 10.3389/fimmu.2020.00400. PMID: 32218786; PMCID: PMC7078160
The cytotoxicity of epitope-specific CD8+ T cells is usually measured indirectly through IFNγ production, but direct measurement assays have historically been limited in multiplexing capacity. ... Here, we develop a multiplex cytotoxicity assay that allows direct, simultaneous measurement of up to 23 different specificities of CD8+ T cells in a single reaction. ... This technique is highly useful for studying both human and mouse CD8+ T cells, providing new possibilities for comprehensive analyses of CD8+ T cell cytotoxicity in vaccine evaluations.
(Tags: anti-mouse CD8b flow cytometry; CD8 T cell cytotoxicity assay)
For more technical references, pricing, or data sheets regarding the Recombinant Anti-Mouse CD8b Monoclonal Antibody (Clone: YTS 156.7) formats, please contact our scientific support team at message@sydlabs.com.

Related Recombinant IgG Reference Antibodies:
In vivo grade recombinant rat IgG2b isotype control antibody

Syd Labs provides the following recombinant anti-human CD8a monoclonal antibodies:
In vivo grade recombinant anti-human CD8a antibodies (clone OKT8)
Recombinant anti-human CD8a antibodies (clone OKT8) for flow cytometry

Syd Labs provides the following recombinant anti-mouse CD8a monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8a antibodies (clone 2.43)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 169.4)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 105.18)

Syd Labs provides the following recombinant anti-mouse CD8b monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8b antibodies (clone YTS 156.7)

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