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Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Our Recombinant Anti-Mouse CD8a Monoclonal Antibody (Clone: 53-6.72) represents a sequence-defined, mammalian-expressed technological upgrade specifically engineered to eliminate the inherent biological vulnerabilities of legacy hybridoma-derived reagents. This premium In Vivo Grade Anti-Mouse CD8a Antibody precisely targets the mouse CD8a protein (T-cell surface glycoprotein CD8 alpha chain), a critical orchestrator of cell-mediated immune defense and T-cell ontogeny. By leveraging advanced recombinant engineering, our Anti-Mouse CD8a Clone 53-6.72 retains the exact, high-fidelity variable regions or complete amino acid sequences of the classic rat IgG2a kappa clone. Unlike traditional hybridoma lines that are notoriously prone to genetic drift, unexpected mutations, and contamination with non-specific endogenous light chains, this recombinant mouse CD8a antibody guarantees absolute batch-to-batch consistency and structural purity, ensuring unparalleled reproducibility in long-term screening campaigns.
In functional immunology and oncology modeling, the classic Anti-CD8a Clone 53-6.7 has established itself as the gold-standard workflow for targeting malignant pathways and executing in vivo CD8a+ T cell depletion assays. However, introducing external monoclonal reagents into live animal models demands stringent safety thresholds to prevent catastrophic non-specific immune activation or subject mortality. To address this paradigm, Syd Labs subjects this low endotoxin anti-mouse CD8a antibody to meticulous purification processes, enforcing ultra-low endotoxin specifications and a complete absence of problematic protein aggregates. By explicitly aligning superior batch reproducibility with uncompromising in vivo safety, our recombinant platform ensures that your in vivo cell depletion and T-cell tracking profiles generate clean, publication-ready datasets, entirely free from confounding systemic side effects.
Our In Vivo Grade Anti-Mouse CD8a Antibody (Clone: 53-6.72) is specifically formulated and optimized for functional in vivo CD8a+ T cell depletion in live models. For analytical applications such as cell surface staining, immunophenotyping, or tissue mapping, we highly recommend our dedicated Recombinant Anti-Mouse CD8a Antibody for Flow Cytometry. This FACS-optimized alternative offers Human IgG1 Fc Silent™ and Biotinylated Avi-Tag formats to minimize background noise and ensure pristine FACS gating profiles.
Recombinant rat IgG2a Monoclonal Antibody.
Synonyms: CD8a, CD8 alpha, Lyt-2, Lyt2 alpha, Ly-2.
Clone: 53-6.72.
Isotype: Rat IgG2a kappa.
Source: The anti-mouse CD8a monoclonal antibody (clone: 53-6.72) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 53-6.72) specifically binds to mouse CD8a.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD8a protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
RRID: AB_3751529
Shipping: The in vivo grade recombinant anti-mouse CD8a monoclonal antibody of clone 53-6.72 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Yes. Our In Vivo Grade Anti-Mouse CD8a Antibody is thoroughly validated for systemic administration to achieve comprehensive, targeted in vivo cd8 depletion clone 53-6.7 equivalent results in murine models. Standard published protocols typically utilize an intraperitoneal (i.p.) injection of 100 µg to 500 µg of the purified antibody per mouse. This dosing regimen effectively ablates cytotoxic T cell populations within peripheral blood, spleen, and lymph nodes for multiple days, making it an ideal choice for immune-mediated infection modeling and oncology screenings.
For an optimal mouse cd8+ t cell depletion protocol, we recommend an initial clone 53-6.7 injection dosage in vivo (utilizing our recombinant 53-6.72 format) ranging from 100 µg to 250 µg per mouse via intraperitoneal (i.p.) administration. Because this antibody features a highly effective rat igg2a anti mouse cd8 depletion isotype, it successfully triggers host Fc-mediated effector functions (such as ADCC and CDC) to clear target cells. Maintenance doses of 100 µg can be administered every 2-3 days depending on your experimental window. We advise verifying depletion efficiency via flow cytometry of peripheral blood or spleen 48-72 hours post-injection.
Traditional hybridoma lines are highly susceptible to genetic drift, chromosomal loss, or contamination with non-specific endogenous myeloma light chains. Our Recombinant Anti-Mouse CD8a Monoclonal Antibody is sequence-defined and expressed under strict serum-free mammalian cell culture conditions. This cutting-edge platform guarantees 100% batch-to-batch structural integrity, superior reproducible binding profiles, and eliminates unwanted non-specific background signals, allowing long-term research cohorts to generate pristine, publication-ready datasets.
To prevent lethal cytokine storms or non-specific physiological inflammation in live subjects, our premium low endotoxin anti-mouse cd8 53-6.7 equivalent antibody undergoes stringent multi-step purification protocols. It features a guaranteed ultra-low endotoxin threshold (<1 EU/mg) and is formulated as a 0.2 µM filtered solution in 1x PBS. The composition is entirely free of sodium azide, carrier proteins, or stabilizing preservatives, meeting the strict requirements for clean, toxicity-free animal injections.
Biochemically and functionally, there is no structural difference in antigen recognition between the two names. The designation 53-6.72 represents the complete cloned subline nomenclature of the classic Anti-CD8a Clone 53-6.7. Our advanced Anti-Mouse CD8a Clone 53-6.72 possesses the exact high-fidelity variable domains that specifically bind the mouse CD8 alpha chain. It seamlessly substitutes for any academic or industrial protocol historically established with the 53-6.7 designation while bringing the upgraded stability of a recombinant expression framework.
Yes. Syd Labs specializes in high-yield bioprocess scale-up to support translational medicine and large-scale industrial platforms. We provide customized, bulk-packaged aliquots of our Recombinant Mouse CD8a Antibody upon request. Additionally, we provide professional engineering options including specific species/isotype chimerization, targeted Fc silencing (e.g., LALAPG mutants to abolish non-specific Fc-receptor engagement), recombinant affinity tagging, and direct enzyme or fluorophore conjugations.
Syd Labs provides the following recombinant anti-human CD8a monoclonal antibodies:
In vivo grade recombinant anti-human CD8a antibodies (clone OKT8)
Recombinant anti-human CD8a antibodies (clone OKT8) for flow cytometry
Syd Labs provides the following recombinant anti-mouse CD8a monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8a antibodies (clone 2.43)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 169.4)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 105.18)
Syd Labs provides the following recombinant anti-mouse CD8b monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8b antibodies (clone YTS 156.7)
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