Anti-Mouse CD8a Monoclonal Antibodies

Background of Anti-mouse CD8a Monoclonal Antibody

The rat anti-mouse CD8a monoclonal antibody 53-6.72  (rat IgG2a kappa) reacts with the mouse CD8a protein (T-cell surface glycoprotein CD8 alpha chain) encoded by the mouse CD8A gene that encodes the CD8a chain of the dimeric CD8 protein. The mouse CD8 protein is primarily responsible for cell-mediated immune defense and T-cell development. CD8A has been widely reported as a potential prognosis and diagnostic marker for several diseases, such as inflammatory disorders and tumors. It was shown that the anti-mouse CD8a rat monoclonal antibody (clone number: 53-6.72) can be used for depleting activity in vivo.

Our recombinant 53-6.72 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse CD8a monoclonal antibody (hybridoma clone name or number: 53-6.72).

PA007533.r2a: In Vivo Grade Recombinant Anti-mouse CD8a Monoclonal Antibody (Clone: 53-6.72), Rat IgG2a Kappa

Recombinant rat IgG2a Monoclonal Antibody.
Clone: 53-6.72.
Isotype: Rat IgG2a kappa.
Source: The anti-mouse CD8a monoclonal antibody (clone: 53-6.72) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 53-6.72) specifically binds to mouse CD8a.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD8a protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The in vivo grade recombinant anti-mouse CD8a monoclonal antibody of clone 53-6.72 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-mouse CD8a Monoclonal Antibody:

Animal model of respiratory syncytial virus: CD8+ T cells cause a cytokine storm that is chemically tractable by sphingosine-1-phosphate 1 receptor agonist therapy
Walsh, K. B., et al. J Virol. 2014 Jun;88(11):6281-93. doi: 10.1128/JVI.00464-14. PMID: 24672024
Isolated cells were stained with antibodies raised against murine CD11b (M1/70), CD11c (HL3), Ly6C (HK1.4), Ly6G (1A8), F480 (BM8), NK1.1 (PK136), CD3e (145-2C11), CD4 (L3T4), CD8a (53-6.7), CD90.2 (53-2.1), B220 (RA3-6B2), CD103 (M290), CD69 (HI.2F3), CD44 (IM7), CD40 (MH40-3), CD80 (16-10A1), CD86 (GL1), PD-L1 (MIH5), H-2Kb (AF6-88.5), and I-A/I-E (M5/114.15.2). ...For cell depletion experiments, 250 ?g of anti-NK1.1 (PK136; Bioxcell) and/or 500 ?g of anti-CD8 (53-6.72; Bioxcell) antibodies were administered i.p. to mice 1 day before and 1 day after infection. ...Lung and mesenteric lymph nodes (MLN) were harvested from mice perfused with ?10 ml of sterile phosphate-buffered saline (PBS). ...Cytokine production by NK cells and CD8+ T cells within the lung on day 5 after PVM infection was determined by 5 h of incubation of single-cell suspensions in culture medium with 4 ?g/ml brefeldin A (Sigma, St. Louis, MO, USA) in the absence of exogenous stimulation. ...Intracellular granzyme B (Life Technologies, Carlsbad, CA, USA) staining was performed directly ex vivo in the absence of stimulation or incubation with brefeldin A.
Tags: anti-mouse CD8a 53-6.72; anti-mouse CD8a 53-6.72 antibody

Clonal expansions of CD8+ T cells with IL-10 secreting capacity occur during chronic Mycobacterium tuberculosis infection.
Cyktor, J. C., et al. PLoS One. 2013;8(3):e58612. doi: 10.1371/journal.pone.0058612. PMID: 23472214
However, the importance of CD8+ T cells during Mtb infection in this mouse strain remains unclear. ...CD8+ T cells are an important component of the protective immune response to Mtb, as defined by studies showing that mice deficient in CD8+ T cells had impaired control of Mtb infection. ...In this study we show that Mtb-susceptible CBA/J mice accumulated large numbers of CD8+ T cells in their lungs as Mtb infection progressed that could not be fully accounted for by an expansion of IFN-?-producing CD8+ T cells. ...Although V?8 CD8+ T cells could secrete IL-10, in vivo depletion of this specific T cell clonal population during chronic infection did not overtly change the Mtb burden in the lungs in the timeframe tested, although the amount of IL-10 in the lung was reduced indicating some biological impact of depletion. ...Anti-CD8+ (53.6.72) depletion antibody (BioXCell) or whole rat IgG2a (2A3) (BioXCell), were diluted to 2.5 mg/ml in PBS and stored at ?80°C.
Tags: anti-mouse CD8a 53-6.72 mAb; anti-mouse CD8a 53-6.72 antibody in vivo

The CTLA-4 and PD-1/PD-L1 inhibitory pathways independently regulate host resistance to Plasmodium-induced acute immune pathology
Hafalla, J. C. R., et al. PLoS Pathog. 2012 Feb;8(2):e1002504. doi: 10.1371/journal.ppat.1002504. PMID: 22319445
Finally, reminiscent of what is known for the susceptible strain, we confirmed that certain T cells (CD8+) and molecules (IFN-?) are crucial to the development of cerebral malaria in the otherwise resistant mice. ...For example, expression of PD-1 is associated with progressive loss of CD8+ T cell effector function during persistent lymphocytic choriomeningitis virus (LCMV) infection in mice; blockade of PD-1/PD-L1 interactions but not the CTLA-4 pathway augmented T cell function and allowed the virus to be controlled. ...High levels of CTLA-4 expression are found on HIV-specific CD4+ T cells, but not on CD8+ T cells, and in vitro blockade of CTLA-4 enhances HIV-specific CD4+ T cell function. ...While these studies clearly indicate a role for the PD-1/PD-L1 pathway in dampening T cell responses, there is, rather confusingly, some evidence that this pathway is important in promoting CD8+ T cell responses in murine influenza virus [32] and Listeria monocytogenes [33] infections, suggesting that the outcome of PD-1/PD-L1 interactions might be modified by other regulatory pathways. ...The current best model of the pathogenesis of ECM is that CD8+ T cells damage cerebral vascular endothelial cells and the underlying basement membrane, thereby breaching the blood brain barrier, causing haemorrhage and oedema.
Tags: anti-mouse CD8a 53-6.72 in animal model; anti-mouse CD8a 53-6.72 in cancer research

For more references about Anti-mouse CD8a Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following recombinant anti-human CD8a monoclonal antibodies:
In vivo grade recombinant anti-human CD8a antibodies (clone OKT8)
Recombinant anti-human CD8a antibodies (clone OKT8) for flow cytometry

Syd Labs provides the following recombinant anti-mouse CD8a monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8a antibodies (clone 2.43)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 169.4)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 105.18)

Syd Labs provides the following recombinant anti-mouse CD8b monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8b antibodies (clone YTS 156.7)