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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2a Monoclonal Antibody.
Clone: 116-13.1.
Isotype: Mouse IgG2a kappa.
Source: The anti-mouse CD8 (Lyt 2.1) monoclonal antibody (clone: 116-13.1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 116-13.1) specifically binds to mouse CD8 (Lyt 2.1) .
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD8 (Lyt 2.1) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD8 (Lyt 2.1) monoclonal antibody of clone 116-13.1 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Binary outcomes of enhancer activity underlie stable random monoallelic expression.
Nagano T, et al. (2022). eLife. 2022 May 26;11:e74204. doi: 10.7554/eLife.74204. PMID: 35617021
anti-CD8.1 (116?13.1) unconjugated, anti-CD8.2 (2.43) unconjugated. Klra1 Hss1? mice were generated by microinjection, while Klrc1 5?E?, Klrk1 5?E? and Klra7 Hss1? mice were generated by CRISPR-EZ electroporation. Founder mice (F0) harboring deletion alleles were backcrossed to C57BL/6 J (B6) mice to generate heterozygous F1 mice, and were then intercrossed to generate WT, heterozygous and homozygous littermates for experiments. F1 hybrid mice between B6-Klrc1 5?E? mice and BALB/c mice were generated (Klrc1 B6-5?E?/BALB/c+ heterozygotes) along with WT F1 littermates.
Tags: 116-13.1 antibody; anti-mouse CD8 116-13.1
Salp15, an Ixodes scapularis salivary protein, inhibits CD4(+) T cell activation.
Garg R, et al. (2002). J Immunol. 2002 Jul 1;169(1):418-24. doi: 10.4049/jimmunol.169.1.418. PMID: 12121666
Mice were depleted of CD8+ T cells by i.p. injection of 500 ?g anti-CD8 mAb (clone 116-13.1) on days ?1, 1, and 7 relative to immunization. Splenocytes from immunized mice were harvested 7 days after the last immunization. CD4+ T cells were purified using magnetic bead separation. Proliferation was measured by [3H]thymidine incorporation after 72 hours. Cytokine levels in culture supernatants were determined by ELISA.
Tags: anti-mouse Lyt 2.1; anti-mouse CD8 116-13.1 antibody
Amplification of tumor immunity by gene transfer of the co-stimulatory molecule B7.
Guha-Thakurta N, et al. (1998). Eur J Immunol. 1998 Mar;28(3):1116-25. doi: 10.1002/(SICI)1521-4141(199803)28:03<1116::AID-IMMU1116>3.0.CO;2-A. PMID: 9541607
Purified mAb 116-13.1 (anti-CD8) was administered i.p. at 200 ?g per mouse to deplete CD8+ T cells. Tumor cells were injected subcutaneously into syngeneic mice. Tumor growth was monitored twice weekly with calipers. Survival of mice was recorded daily. Histological analysis of tumors was performed on day 14 post-inoculation.
Tags: anti-mouse CD8 116-13.1 in vivo; anti-mouse CD8 116-13.1 in animal model
CD8 surface levels alter the fate of alpha/beta T cell receptor-expressing thymocytes in transgenic mice.
Swat W, et al. (1992). J Exp Med. 1992 Apr 1;175(4):1013-22. doi: 10.1084/jem.175.4.1013. PMID: 1532412
Anti-CD8 monoclonal antibody (clone 116-13.1) was injected intraperitoneally into pregnant females at 100 ?g per dose. Thymocytes were analyzed by flow cytometry 48 hours post-injection. Transgenic mice were crossed to generate double transgenics. Peripheral T cell numbers were enumerated in spleen and lymph nodes. Positive and negative selection was assessed in thymic sections.
Tags: anti-mouse CD8 116-13.1 mAb in cancer research; function of anti-mouse CD8 116-13.1
Human papillomavirus type 16 nucleoprotein E7 is a tumor-rejection antigen.
Chen L, et al. (1991). Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):110-4. doi: 10.1073/pnas.88.1.110. PMID: 1846033
Depletion of CD8+ T cells was achieved by i.p. administration of mAb 116-13.1 (200 ?g/mouse) three days before tumor challenge. Mice were immunized with E7-expressing vaccinia virus. Tumor regression was observed in 80% of immunized mice. Control mice received parental vaccinia. CTL activity was measured using 51Cr release assay.
Tags: bioactivity of anti-mouse CD8 116-13.1; anti-mouse Lyt 2.1 116-13.1
For more references about anti-mouse CD8 antibody (116-13.1), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant anti-human CD8a monoclonal antibodies:
In vivo grade recombinant anti-human CD8a antibodies (clone OKT8)
Recombinant anti-human CD8a antibodies (clone OKT8) for flow cytometry
Syd Labs provides the following recombinant anti-mouse CD8a monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8a antibodies (clone 2.43)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 169.4)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 105.18)
Syd Labs provides the following recombinant anti-mouse CD8b monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8b antibodies (clone YTS 156.7)
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