Anti-Mouse CD8a Monoclonal Antibodies for Flow Cytometry

PA007497.r2b: Recombinant Anti-mouse CD8a Monoclonal Antibody (Clone: YTS 169.4), Rat IgG2b Kappa

Recombinant Rat IgG2b Monoclonal Antibody. 
Clone: YTS 169.4.
Isotype:  Rat IgG2b Kappa.
Source: The anti-mouse CD8a monoclonal antibody (clone: YTS 169.4) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant YTS 169.4 antibody specifically binds to mouse CD8a protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007497.h1Fs: Recombinant Anti-mouse CD8a Monoclonal Antibody (Clone: YTS 169.4), Human IgG1 Fc Silent Kappa

Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: YTS 169.4.
Isotype: Human IgG1 kappa.
Source: The anti-mouse CD8a monoclonal antibody (clone: YTS 169.4) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant YTS 169.4 antibody specifically binds to mouse CD8a protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The YTS 169.4 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.

Background

The rat anti-mouse CD8a monoclonal antibody YTS 169.4 (rat IgG2b kappa) reacts with the mouse CD8a protein (T-cell surface glycoprotein CD8 alpha chain) encoded by the mouse CD8A gene that encodes the CD8a chain of the dimeric CD8 protein. The mouse CD8 protein is primarily responsible for cell-mediated immune defense and T-cell development. CD8A has been widely reported as a potential prognosis and diagnostic marker for several diseases, such as inflammatory disorders and tumors. It was shown that the anti-mouse CD8a rat monoclonal antibody (clone number: YTS 169.4) can be used for depleting activity in vivo.
Our recombinant YTS 169.4 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse CD8a monoclonal antibody (hybridoma clone name or number: YTS 169.4).

References of anti-mouse CD8a antibody (YTS 169.4)

Immunological correlates of protection mediated by a whole-cell pertussis vaccine
Diavatopoulos DA, et al. (2024). mBio. 2024 Aug 14;15(8):e0174624. doi: 10.1128/mbio.01746-24. PMID: 39118232
For CD8+ T-cell depletion, mice were injected intraperitoneally with 100 µg of anti-CD8 antibody (clone YTS 169.4) on days -3, 0, and every 3–4 days thereafter. ... Flow cytometry was used to confirm the depletion of CD8+ T cells in spleen and lung tissues. ... The whole-cell pertussis vaccine induced robust Th1 and Th17 responses in vaccinated mice. ... Protection against Bordetella pertussis was assessed by measuring bacterial loads in the lungs. ... Immunological correlates were evaluated using intracellular cytokine staining and flow cytometry.
Tags: activity of anti-mouse CD8a antibody; anti-mouse CD8a antibody for animal model

Endocytic membrane repair by ESCRT-III controls antigen export to the cytosol during cross-presentation
Cebrian I, et al. (2022). Elife. 2022 Aug 23;11:e78511. doi: 10.7554/eLife.78511. PMID: 36005844
For CD8 T cell depletion, 100 µg of YTS 169.4 antibody was injected intraperitoneally 3 days before and on the day of immunization. ... Flow cytometry was performed to quantify the ratio of blue to green fluorescence as a measure of antigen export to the cytosol. ... Bone marrow-derived dendritic cells were pulsed with ovalbumin and analyzed for cross-presentation efficiency. ... ESCRT-III machinery was critical for maintaining endocytic membrane integrity during antigen processing. ... The study confirmed that CD8+ T cell responses were dependent on cytosolic antigen presentation.
Tags: anti-mouse CD8a for cancer research; anti-mouse CD8a for mouse tumor model

Inhibiting myeloid-derived suppressor cell trafficking enhances T cell immunotherapy
Sun L, et al. (2019). JCI Insight. 2019 Apr 18;4(8):e126853. doi: 10.1172/jci.insight.126853. PMID: 31011208
For CD8+ T cell depletion, 100 µg of anti-CD8 antibody (clone YTS 169.4) was administered intraperitoneally on days -3, 0, and every 3–4 days post-tumor implantation. ... Flow cytometry was used to evaluate the infiltration of myeloid-derived suppressor cells in the tumor microenvironment. ... Inhibition of MDSC trafficking enhanced the efficacy of anti-PD-1 immunotherapy. ... Tumor growth was monitored using caliper measurements in syngeneic mouse models. ... T cell activation was assessed by staining for CD69 and IFN-γ expression.
Tags: anti-CD8a monoclonal antibody; function of anti-mouse CD8a antibody

CD8+ T Cell Response to Gammaherpesvirus Infection Mediates Lymphoproliferative Disease
Mishra A, et al. (2015). PLoS One. 2015 Aug 7;10(8):e0135719. doi: 10.1371/journal.pone.0135719. PMID: 26241984
Mice were depleted of CD8+ T cells by intraperitoneal injection of 100 µg of YTS 169.4 antibody on days -3 and 0 relative to infection. ... Flow cytometry was used to analyze CD8+ T cell responses in spleens of gammaherpesvirus-infected mice. ... Lymphoproliferative disease was assessed by histological examination of spleen and lymph nodes. ... Viral load was quantified using qPCR in infected tissues. ... Depletion of CD8+ T cells reduced the severity of lymphoproliferative disease.
Tags: YTS 169.4 mAb for flow cytometry; anti-mouse CD8a YTS 169.4 for flow cytometry

Early expression of interferon-γ inducible protein 10 and monokine induced by interferon-γ in cardiac allografts is mediated by CD8+ T cells
Kapoor A, et al. (2000). Transplantation. 2000 Mar 27;69(6):1147-55. doi: 10.1097/00007890-200003270-00020. PMID: 10760720
To deplete CD8+ T cells, 0.5 mg of anti-CD8 monoclonal antibody (YTS 169.4) was injected intraperitoneally on days -3 and -1 before transplantation. ... Flow cytometry was performed on spleen cell suspensions to confirm CD8+ T cell depletion. ... Chemokine expression (IP-10 and Mig) was assessed in cardiac allografts using RT-PCR. ... CD8+ T cells were found to be critical mediators of early chemokine expression. ... Allograft rejection was monitored by palpation and histological analysis.
Tags: anti-mouse CD8a YTS 169.4 mAb for flow cytometry; anti-mouse CD8a YTS 169.4

For more references about anti-mouse CD8a antibody (YTS 169.4), please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following recombinant anti-human CD8a monoclonal antibodies:
In vivo grade recombinant anti-human CD8a antibodies (clone OKT8)
Recombinant anti-human CD8a antibodies (clone OKT8) for flow cytometry

Syd Labs provides the following recombinant anti-mouse CD8a monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8a antibodies (clone 2.43)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 169.4)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 105.18)

Syd Labs provides the following recombinant anti-mouse CD8b monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8b antibodies (clone YTS 156.7)