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Recombinant rat IgG2b isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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The rat anti-mouse CD8a monoclonal antibody clone YTS 105.18 specifically reacts with the mouse CD8a protein (T-cell surface glycoprotein CD8 alpha chain) encoded by the mouse CD8A gene. While some alternative suppliers and historical researchers mistakenly misidentify the YTS 105.18 clone as a rat IgG2a antibody, heavy chain variable region sequence validation strictly confirms that its true biological isotype is rat IgG2b kappa. In immunological research, the CD8 protein is a critical marker for cell-mediated immune defense and T-cell development, serving as a pivotal diagnostic and prognostic indicator in inflammatory disorder and tumor profiling datasets.
Syd Labs' recombinant anti-mouse CD8a antibody for flow cytometry (clone YTS 105.18) is engineered to deliver exceptional batch-to-batch consistency and high signal-to-noise ratios. Because clone YTS 105.18 functionally acts as a non-depleting anti-mouse CD8a reagent?blocking receptor interactions without triggering systemic cell death?it represents the gold-standard framework for Flow Cytometry (FC/FACS) applications that require viable cell surface staining and artifact-free FACS sorting. Available in multiple highly purified formats, including native rat IgG2b, advanced human IgG1 fc silent engineered variants to completely eradicate non-specific Fc-receptor binding, and biotinylated anti-mouse cd8 clone yts 105.18 conjugates, this product pipeline is meticulously optimized to integrate seamlessly into complex multi-color flow cytometry panels.
Recombinant Rat IgG2b Monoclonal Antibody.
Clone: YTS 105.18.
Isotype: Rat IgG2b Kappa.
Source: The anti-mouse CD8a monoclonal antibody (clone: YTS 105.18) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant YTS 105.18 antibody specifically binds to the mouse CD8a protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: YTS 105.18.
Isotype: Human IgG1 kappa.
Source: The anti-mouse CD8a monoclonal antibody (clone: YTS 105.18) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant YTS 105.18 antibody specifically binds to mouse CD8a protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant human IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: YTS 105.18.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated anti-mouse CD8a monoclonal antibody (clone: YTS 105.18) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant YTS 105.18 antibody specifically binds to the mouse CD8a protein.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The YTS 105.18 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
While both clones target the mouse T-cell surface glycoprotein CD8 alpha chain, they serve completely distinct experimental purposes. Clone 2.43 is primarily a depleting antibody widely utilized for in vivo T-cell clearance. Conversely, clone YTS 105.18 is functionally non-depleting. For flow cytometry (FC/FACS) and live-cell sorting, Syd Labs' recombinant clone YTS 105.18 is the optimal choice because it binds and stains CD8a+ cells efficiently without triggering systemic cell clearance or antibody-dependent cellular cytotoxicity (ADCC), ensuring high cell viability post-sorting.
Traditional hybridoma-derived antibodies often exhibit non-specific binding to Fcγ receptors (FcγR) expressed on immune cells (such as macrophages, B cells, and NK cells), leading to false-positive signals and high background fluorescence in multi-color flow cytometry. Syd Labs' recombinant human IgG1 Fc silent engineered framework incorporates specific mutations within the Fc domain to completely abolish its binding affinity to Fc receptors. This engineered Fc silent anti-mouse CD8 antibody eliminates the need for extensive Fc-blocking steps, significantly enhancing the signal-to-noise ratio and delivering precise gating for sensitive multicolor flow panels.
Using a biotinylated anti-mouse CD8 clone YTS 105.18 antibody provides a highly sensitive, flexible detection method when paired with fluorochrome-conjugated streptavidin in multi-color flow panels. Syd Labs' biotin avi tag formats utilize site-specific, enzymatically controlled biotinylation during recombinant production. Unlike harsh chemical biotinylation which randomly targets lysine residues and can compromise the antibody's antigen-binding site, the Avi-tag ensures that every single antibody molecule is uniformly biotinylated at a unique peptide sequence. This preserves maximum binding affinity and yields exceptional batch-to-batch consistency in flow cytometric staining.
There is widespread misinformation in some supplier catalogs and older literature stating that clone YTS 105.18 is a rat IgG2a. However, precise heavy chain variable region sequence validation confirms that the true biological isotype of clone YTS 105.18 is rat IgG2b kappa. Syd Labs strictly adheres to sequence-verified recombinant production standards, ensuring that our native formats match the authentic rat IgG2b sequence, allowing you to select the absolute correct isotype control antibody for your flow cytometry controls.
Syd Labs provides the following in vivo grade recombinant anti-mouse CD8 monoclonal antibodies:
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: 2.43)
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: YTS 169.4)
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: YTS 105.18)
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: 53-6.72)
Recombinant Anti-mouse CD8b monoclonal antibody (Clone: YTS 156.7)
Recombinant Anti-mouse CD8 (Lyt 2.1) monoclonal antibody (Clone: 116-13.1)
Syd Labs provides the following in vivo grade recombinant anti-human CD8 monoclonal antibodies:
Recombinant Anti-human CD8a monoclonal antibody (Clone: OKT8)
Syd Labs provides the following recombinant anti-mouse CD8 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: 2.43) for flow cytometry
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: YTS 169.4) for flow cytometry
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: YTS 105.18) for flow cytometry
Recombinant Anti-mouse CD8b monoclonal antibody (Clone: YTS 156.7) for flow cytometry
Syd Labs provides the following recombinant anti-human CD8 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD8a monoclonal antibody (Clone: OKT8) for flow cytometry
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