Anti-Mouse CD4 Monoclonal Antibodies

PA007200.r2b: In vivo Grade Recombinant Anti-mouse CD4 Rat IgG2b Kappa Monoclonal Antibody (Clone: GK1.5)

Recombinant rat IgG2b Monoclonal Antibody.
Clone: GK1.5.
Isotype: Rat IgG2b kappa.
Source: The anti-mouse CD4 monoclonal antibody (clone: GK1.5) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: GK1.5) specifically binds to the mouse T cell receptor CD4.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD4 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-mouse CD4 Monoclonal Antibody:

Macrophage Migration Inhibitory Factor protects cancer cells from immunogenic cell death and impairs anti-tumor immune responses
Balogh, K. N., et al. PLoS One. 2018 Jun 4;13(6):e0197702. doi: 10.1371/journal.pone.0197702. PMID: 29864117
Beginning two days before 4T1 cell tumor implantation, mice were treated with intraperitoneal injection of an initial dose of 200ug/mouse of anti-CD4 (clone GK1.5, BioXCell) and anti-CD8 (clone 2.43, BioXCell) antibodies in PBS, followed by similar dosing with 100ug/mouse every 4 days throughout the course of tumor growth. ...The MIF KD tumors also contained significantly fewer CD4+ T cells by percent. ...However, no significant difference was observed in the total number of CD4+ T cells. ...Interestingly, both populations of tumor-infiltrating T cells in the MIF KD tumors were more activated, as exhibited by the ability of both CD8+ and CD4+ T cells to express IFNgamma after ex vivo re-stimulation. ...Therefore, we depleted the CD4 and CD8 T cells simultaneously in the same mice.
Tags: anti-mouse CD4 GK1.5; anti-mouse CD4 GK1.5 mAb

TGF? Is a Master Regulator of Radiation Therapy-Induced Antitumor Immunity
Vanpouille-Box, C., et al. Cancer Res. 2015 Jun 1;75(11):2232-42. doi: 10.1158/0008-5472.CAN-14-3511. PMID: 25858148
Depletion of CD4+ and CD8+ T cells was achieved by injecting GK1.5 or 2.43 mAb (BioXCell) given i.p. at 100 ?g/mouse on 3 consecutive days, starting at day 10, and was maintained by weekly injections. ...Briefly, TDLN cells were stained with anti-mouse CD4-PE and anti-mouse CD8-FITC (eBioscience), fixed, permeabilized (Foxp3 Fixation/Permeabilization Concentrate and Diluent kit, eBioscience), and stained with goat anti-phospho-Smad2/3 (Ser 423/425) followed by APC-labeled donkey anti-goat IgG (Santa Cruz Biotechnology). ...Images were obtained using a Leica SNC400F fluorescence slide scanner. CD4 and CD8 T cells were counted in five randomly selected (×200) fields in each tumor. ...TIDC, defined as CD45+CD11c+MHC-II+ cells, was analyzed at day 22 for expression of activation markers CD40 and CD70, which plays a critical role in controlling priming versus tolerance induction of CD8+ T cells. ...Consistent with this, depletion of CD4+ or CD8+ T cells abrogated regression of the irradiated 4T1 tumors and restored the number of lung metastases in mice treated with TGF? inhibition + RT.
Tags: anti-mouse CD4 GK1.5 antibody in vivo; anti-mouse CD4 GK1.5 mAb in vivo

Memory programming in CD8(+) T-cell differentiation is intrinsic and is not determined by CD4 help
Kim, J., et al. Nat Commun. 2015 Aug 14:6:7994. doi: 10.1038/ncomms8994. PMID: 26272364
Stimulation of CD8+ T cells in the absence of CD4+ T-cell help is an important constraint on the quantity and quality of the CD8+ T-cell response, resulting in defects in memory expansion of activated CD8+ T cells. ...The general consensus is that CD4 help delivered during CD8+ T-cell priming encodes a programme in the activated CD8+ T cells to generate memory cells. ...Nevertheless, what is the fundamental role of CD4+ T cells in preventing memory impairment of CD8+ T cells remains to be elucidated. ...However, the reliance on contrived approaches to create helper deficiency, such as CD4 depletion and the use of major histocompatibility complex (MHC) II- or CD4-deficient mice, and the paucity of antigen-specific CD8+ T cells expanded after helper-deficient activation limit extrapolating these results to physiological situations. ...However, this immunodominance is CD4+ T-helper cell-dependent.
Tags: anti-mouse CD4 GK1.5 antibody in animal model; anti-mouse CD4 GK1.5 mAb in animal model

IL-27 Signaling Is Crucial for Survival of Mice Infected with African Trypanosomes via Preventing Lethal Effects of CD4+ T Cells and IFN-?
Liu, G., et al. PLoS Pathog. 2015 Jul 29;11(7):e1005065. doi: 10.1371/journal.ppat.1005065. PMID: 26222157
Either depletion of CD4+ T cells, resulting in a dramatically reduced secretion of IFN-?, or neutralization of IFN-?, prevented the early mortality of infected IL-27R-/- mice with a significantly reduced inflammatory response and a major amelioration of the liver pathology. ...Thus, our data identify IL-27 signaling as a novel pathway to prevent the early mortality via inhibiting hyperactivation of CD4+ Th1 cells and their excessive secretions of IFN-? during experimental infection with extracellular protozoan parasites African trypanosomes. ...More recent studies have suggested that IL-27 also has the function to inhibit immunopathology via downregulation of active CD4+ T cells during infections, particularly with intracellular protozoan parasites. ...However, the precise mechanism of CD4+ T cell-mediated immunopathogenesis in the absence of IL-27 signaling still remains incompletely understood. ...A limited and similar percentage and absolute number of CD4+ T cells from uninfected wild-type and IL-27R-/- mice produced IFN-? after 12 h stimulation with Cell Stimulation Cocktail (containing PMA, ionomycin, and protein transport inhibitors).
Tags: anti-mouse CD4 GK1.5 in cancer research; anti-mouse CD4 GK1.5 mAb in cancer research

Antibody Blockade of Semaphorin 4D Promotes Immune Infiltration into Tumor and Enhances Response to Other Immunomodulatory Therapies
Evans, E. E., et al. Cancer Immunol Res. 2015 Jun;3(6):689-701. doi: 10.1158/2326-6066.CIR-14-0171. PMID: 25614511
Other antibody clones, including anti?PD-1/MAb RMP1-14, anti-CD8/MAb 2.43, and anti-CD4/MAb GK1.5, were purchased from BioXCell and were verified to have <2 EU/mg endotoxin levels, ?95% purity, and ?5% high-molecular-weight species before in vivo use. ...Anti-SEMA4D and anti?CTLA-4 demonstrated independent and combinatorial effects on Teff to Treg ratios as determined by FACS analysis (Teff = CD8+/CD69+; Treg = CD4+/CD25+; Fig. 5C and Supplementary Fig. S5A). ...Tumor-associated M2 macrophages can interact with lymphocytes to promote tumor growth; for example, TAMs can regulate the influx of CD4+ Tregs and can inhibit cytotoxic CD8+ T-cell responses through several mechanisms. ...Spleens were dissociated into small pieces and the tissue homogenate was pressed through a 0.07-mm cell strainer, followed by RBC lysis (Bioscience) for 5 minutes at room temperature. ...Splenocytes and TILs were assayed by Cytometric Bead Array (CBA) cytokine analysis.
Tags: anti-mouse CD4 GK1.5 antibody in mouse tumor model; anti-mouse CD4 GK1.5 antibody of low endotoxin

For more references about Anti-mouse CD4 Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-human CD4 monoclonal antibodies:
Clenoliximab biosimilar, research grade, anti-human CD4 monoclonal antibody
Ibalizumab biosimilar, research grade, anti-human CD4 monoclonal antibody
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4)
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A)
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2)
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD4 monoclonal antibodies:
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: GK1.5)

Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a) for flow cytometry