PA007200.r2b: In vivo Grade Recombinant Anti-mouse CD4 Rat IgG2b Kappa Monoclonal Antibody (Clone: GK1.5)
Recombinant rat IgG2b Monoclonal Antibody.
Clone: GK1.5.
Isotype: Rat IgG2b kappa.
Source: The anti-mouse CD4 monoclonal antibody (clone: GK1.5) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: GK1.5) specifically binds to the mouse T cell receptor CD4.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD4 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
References of anti-mouse CD4 antibody (GK1.5)
In vivo administration of Fab' fragments of anti-L3T4 (GK1.5) antibody inhibits the T helper cell function of murine lymph node cells
N E Street, et al. (1989). Cell Immunol. 1989 Apr;120(1):75-81. doi: 10.1016/0008-8749(89)90175-5. PMID: 2522831
The effect of intravenous injection of Fab? fragments of anti-L3T4 antibody (GK1.5 monoclonal antibody) into mice was studied. ... This treatment depleted L3T4 + cells from the popliteal lymph nodes of keyhole-limpet hemocyanin-primed mice. ... The T cells that remained were unable to provide help to antigen-specific B cells in vitro. ... The results obtained using Fab? fragments were comparable with those using intact anti-L3T4 antibody and demonstrate that either form of GK1.5 is a potentially useful immunosuppressive agent in mice. ... This treatment depleted L3T4 + cells from the popliteal lymph nodes of keyhole-limpet hemocyanin-primed mice.
Tags: activity of anti-mouse CD4 antibody; anti-mouse CD4 antibody for animal model
In vivo effects of GK1.5 (anti-L3T4a) monoclonal antibody on induction and expression of delayed-type hypersensitivity
S D Miller, et al. (1985). Cell Immunol. 1985 May;92(2):414-26. doi: 10.1016/0008-8749(85)90022-x. PMID: 2859929
The in vivo effects of monoclonal GK1.5 antibody, directed against the L3T4a determinant expressed on Class II-restricted T cells, on the induction and expression of murine delayed-type hypersensitivity (DTH) responses were examined. ... Development and expression of both hapten (2,4-dinitrofluorobenzene and 2,4,6-trinitrochlorobenzene)- and protein antigen poly(Glu 60Ala 30Tyr 10)-specific DTH are significantly inhibited by injection of monoclonal anti-L3T4a antibody. ... The inhibitory effects of anti-L3T4a were most pronounced when administered during the afferent (induction) phase of the DTH response, leading to the functional inhibition of the generation of both polyclonal lymph node T-proliferative cells (Tprlf) and DTH effector cells (T DH). ... The in vivo inhibitory effect is apparently unrelated to preferential induction of suppressor T cells as GK1.5 inhibited DTH induction in cyclophosphamide-treated as well as normal recipients. ... Possible mechanisms where in vivo injection of anti-L3T4a inhibits Class II-restricted T-cell subsets involved in DTH induction and expression, including immune depletion and inhibition of T-cell-receptor/ligand interactions, are discussed.
Tags: anti-CD4 antibody (GK1.5); clone GK1.5 of anti-mouse CD4
ImmunoPET Imaging of Murine CD4+ T Cells Using Anti-CD4 Cys-Diabody: Effects of Protein Dose on T Cell Function and Imaging
Amanda C Freise, et al. (2017). Mol Imaging Biol. 2017 Aug;19(4):599-609. doi: 10.1007/s11307-016-1032-z. PMID: 27966069
Given the key role of these cells in regulating immunity, it is important to develop a biologically inert probe. GK1.5 cys-diabody (cDb), a previously developed anti-mouse CD4 antibody fragment, was tested at different doses to assess its effects on positron emission tomography (PET) imaging and CD4 + T cell viability, proliferation, CD4 expression, and function. ... For immunoPET imaging, the lowest protein dose of 2 ?g of 89Zr-labeled GK1.5 cDb resulted in significantly higher % injected dose/g in inguinal lymph nodes (ILN) and spleen compared to the 12-?g protein dose. ... In vivo administration of GK1.5 cDb at the high dose of 40 ?g caused a transient decrease in CD4 expression in spleen, blood, lymph nodes, and thymus, which recovered within 3 days postinjection; this effect was reduced, although not abrogated, when 2 ?g was administered. ... Proliferation was inhibited in vivo in ILN but not the spleen by injection of 40 ?g GK1.5 cDb. ... Concentrations of GK1.5 cDb in excess of 25 nM significantly inhibited CD4 + T cell proliferation and interferon-? production in vitro.
Tags: GK1.5 anti-mouse CD4 antibody; GK1.5 mAb in vivo
In vivo use of monoclonal antibodies against murine T cell antigens
R R Ghobrial, et al. (1989). Clin Immunol Immunopathol. 1989 Sep;52(3):486-506. doi: 10.1016/0090-1229(89)90162-1. PMID: 2788053
neither the C5 component of complement nor antibody-dependent cell-mediated cytotoxicity mediated the action of GK1.5 antibody in vivo; ... intraperitoneal compared to intravenous administration of the different antibodies achieved the same serum levels whether in the presence or absence of the recipient's thymus; ... repeated treatment with a rat IgM anti-L3T4 or a rat IgG2b anti-Lyt2 antibody was followed by inability to detect serum levels of each antibody; ... in vivo treatment with these antibodies caused target cell lysis, target antigen masking without cell destruction, or target antigen modulation without cell destruction and the particular effect of a given antibody could not be predicted by its isotype or specificity; ... thymectomy prolonged the effect of most, but not all antibodies.
Tags: GK1.5 mAb for animal model; GK1.5 antibody for mouse tumor model
Autoimmune diabetes in NOD mouse is L3T4 T-lymphocyte dependent
Y Wang, et al. (1987). Diabetes. 1987 Apr;36(4):535-8. doi: 10.2337/diab.36.4.535. PMID: 3102301
L3T4+ T-lymphocytes were depleted by the in vivo administration of the L3T4-specific monoclonal antibody GK1.5. ... L3T4 levels remained at this low level for approximately 2 wk after withdrawal of GK1.5 treatment, after which the L3T4 levels slowly began to increase in the periphery. ... Grafting of cultured BALB/c islet tissue into GK1.5-treated diabetic NOD mice resulted in a rapid return to normoglycemia that persisted for 2-4 wk. ... Cultured BALB/c islets fail to function when transplanted into diabetic nonobese diabetic (NOD) mice; such grafted tissue is rapidly destroyed by disease recurrence. ... The gradual return to the hyperglycemic condition roughly correlated with the reappearance of L3T4+ T-lymphocytes in the peripheral circulation.
Tags: function of GK1.5 mAb; anti-mouse CD4 GK1.5
For more references about anti-mouse CD4 antibody (GK1.5), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-human CD4 monoclonal antibodies:
Clenoliximab biosimilar, research grade, anti-human CD4 monoclonal antibody
Ibalizumab biosimilar, research grade, anti-human CD4 monoclonal antibody
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4)
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A)
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2)
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a)
Syd Labs provides the following in vivo grade recombinant anti-mouse CD4 monoclonal antibodies:
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: GK1.5)
Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a) for flow cytometry