Anti-Mouse CD4 Monoclonal Antibodies for Flow Cytometry

PA007494.r2b: Recombinant Anti-mouse CD4 Monoclonal Antibody (Clone: GK1.5), Rat IgG2b Kappa

Recombinant Rat IgG2b Monoclonal Antibody. 
Clone: GK1.5.
Isotype:  Rat IgG2b Kappa.
Source: The anti-mouse CD4 monoclonal antibody (clone: GK1.5) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant GK1.5 antibody specifically binds to mouse T cell receptor CD4.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007494.h1Fs: Recombinant Anti-mouse CD4 Monoclonal Antibody (Clone: GK1.5), Human IgG1 Fc Silent Kappa

Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: GK1.5.
Isotype: Human IgG1 kappa.
Source: The anti-mouse CD4 monoclonal antibody (clone: GK1.5) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant GK1.5 antibody specifically binds to mouse T cell receptor CD4.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The GK1.5 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.

References of anti-mouse CD4 antibody (GK1.5)

ImmunoPET Imaging of Murine CD4+ T Cells Using Anti-CD4 Cys-Diabody: Effects of Protein Dose on T Cell Function and Imaging
Freise, A. C., et al. (2017). Mol Imaging Biol. 19(4):599-609. doi: 10.1007/s11307-016-1032-z. PMID: 27966069
GK1.5 cys-diabody (cDb), a previously developed anti-mouse CD4 antibody fragment, was tested at different doses to assess its effects on positron emission tomography (PET) imaging and CD4+ T cell viability, proliferation, CD4 expression, and function. In vivo administration of GK1.5 cDb at the high dose of 40 µg caused a transient decrease in CD4 expression in spleen, blood, lymph nodes, and thymus, which recovered within 3 days post-injection; this effect was reduced, although not abrogated, when 2 µg was administered. Concentrations of GK1.5 cDb in excess of 25 nM significantly inhibited CD4+ T cell proliferation and interferon-γ production in vitro. We previously developed an anti-CD4 antibody fragment, GK1.5 cys-diabody (cDb), for immunoPET imaging of murine CD4+ T cells and described its use in monitoring CD4+ T cell reconstitution after hematopoietic stem cell transplantation. To investigate effects of GK1.5 cDb on the expression of CD4 by CD4+ T cells, we injected 40 µg GK1.5 cDb into C57BL/6 mice and measured surface CD4 expression by T cells in blood, spleen, ILN, and thymus after one or three days.
Tags: GK1.5 mAb for flow cytometry; anti-mouse CD4 GK1.5 for flow cytometry

Migrant memory B cells secrete luminal antibody in the vagina
Oh, J.E., et al. (2019). Nature. 571(7763):122-126. doi: 10.1038/s41586-019-1285-1. PMID: 31189952
Cells were surface stained with anti-CD3ε (145–2C11), anti-CD4 antibodies (GK1.5 and RM4–5), and anti-CD44 (IM7), and then fixed and permeabilized using a Cytofix/Cytoperm™ Fixation/Permeabilization kit according to the manufacturer’s instructions. To deplete CD4 T cells, mice were treated with 300 µg intravenously on day −4, −1 and 10 µg anti-mouse CD4 Ab (GK1.5) intravaginally for four consecutive days before virus challenge. Stained cells were sorted by FACS Aria (BD Biosciences, Mountain View, CA). CD45 +CD3ε +CD4 +, CD45 +CD3ε +CD8α +, CD45 +CD3ε −CD19 + cells (vagina) and CD45 +CD19 +IgD −IgG +MHCII +GL7 −CD38 + cells (spleen) were collected for further analysis. Anti-CD45.2 (104), anti-CD45.1 (A20), anti-CD45 (30-F11), anti-CD3ε (145–2C11), anti-CD4 antibodies (GK1.5 and RM4–5), anti-CD8α (53–6.7), anti-CD19 (6D5 and 1D3), anti-CD45R/B220 (RA3–6B2), anti-CD138 (281–2), anti-CD38 (90), anti-GL7 (GL7), anti-MHC class II (I-A/I-E, M5/114.15.2), anti-CD11c (N418), anti-IgD (11–26c.2a), anti-CD44 (IM7), anti-PD-L2 (TY25), anti-CD80 (16–10A1), anti-CD69 (H1.2F3), anti-CD86 (GL1) were purchased from...
Tags: function of anti-mouse CD4 GK1.5 antibody; bioactivity of anti-mouse CD4 GK1.5 mAb

Effect of a CD4-depleting antibody on the development of Cryptococcus neoformans-induced allergic bronchopulmonary mycosis in mice
Arora, S., et al. (2006). Infect Immun. 74(7):4339-48. doi: 10.1128/IAI.01989-05. PMID: 16790808
Mice were treated with 300 μg of anti-CD4 monoclonal antibody (MAb) GK1.5 intraperitoneally on the day prior to C. neoformans inoculation and boosted with 100 μg of MAb at days 7 and 14. The efficiency of T-cell depletion was assessed by flow cytometric analysis using anti-CD4 Ab RM 4-4, which binds to a region of CD4 distinct from GK1.5. Mice were rendered CD4 + T-cell deficient by injecting them intraperitoneally with the anti-CD4 MAb GK1.5. In comparison, anti-CD4 Ab-treated animals had much less inflammation, including fewer macrophages and eosinophils (Fig. 2B). Histological sections of lungs from control and anti-CD4 Ab-treated mice at week 3 postinfection were stained with PAS, which stains mucus pink.
Tags: anti-mouse CD4 [Clone GK1.5] - purified; anti-CD4 antibody (GK1.5)

Protocol for the isolation of mouse senescence-associated CD4+ T cells using flow cytometry and functional assays
Fukushima, Y., et al. (2023). STAR Protoc. 4(3):102472. doi: 10.1016/j.xpro.2023.102472. PMID: 37515759
CD4 MicroBeads do not interfere with anti-CD4 Ab (clone GK1.5) in the staining panels (Tables 1 and 2) and thus are compatible. Anti-CD4 (GK1.5), FITC (1:200)" is listed in the Key resources table under Antibodies. Anti-CD4 (GK1.5), APC-Cy7 (1:200)" is listed in the Key resources table under Antibodies. CD4 | GK1.5 | FITC | 1:200" is listed in Table 1 under Ab staining panel A to isolate CD153+ SA-T cells. CD4 | GK1.5 | APC-Cy7 | 1:200" is listed in Table 2 under Ab staining panel B to isolate “enriched” CD153+ SA-T cells.
Tags: clone GK1.5 of anti-mouse CD4; mouse CD4 antibody (clone GK1.5)

T4+ T helper cell function in vivo: differential requirement for induction of antiviral cytotoxic T-cell and antibody responses
Ahmed, R., et al. (1988). J Virol. 62(6):2102-2106. doi: 10.1128/JVI.62.6.2102-2106.1988. PMID: 2966865
Two monoclonal antibodies (GKI.5 and RL172.4) directed against the L3T4 (T4) molecule were used for depleting T helper cells from mice. The rat anti-mouse hybridoma MAb GK1.5 was obtained from the American Type Culture Collection, Rockville, Md. For in vivo treatment with GK1.5, mice were injected intraperitoneally (i.p.) with a total of 1.5 mg of GK1.5 antibody. Phycoerythrin-conjugated anti-mouse L3T4 MAb (clone GK1.5), biotin-conjugated anti-mouse Lyt2 MAb (clone 53-6), and streptavidin-phycoerythrin were purchased from Becton Dickinson, Mountain View, Calif. Two injections of GK1.5 (300 ,ug each on days -3 and -1) caused >90% depletion of L3T4+ cells.
Tags: purified anti-mouse CD4 (GK1.5); GK1.5 monoclonal antibody

For more references about anti-mouse CD4 antibody (GK1.5), please contact our scientific support team with message@sydlabs.com.