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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG1 isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG1 Monoclonal Antibody.
Clone: SK3 / Anti-Leu 3a.
Isotype: Mouse IgG1 kappa.
Source: The anti-human CD4 monoclonal antibody (clone: SK3 / Anti-Leu 3a) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant SK3 / Anti-Leu 3a antibody specifically binds to human CD4.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: SK3 / Anti-Leu 3a.
Isotype: Human IgG1 kappa.
Source: The anti-human CD4 monoclonal antibody (clone: SK3 / Anti-Leu 3a) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant SK3 / Anti-Leu 3a antibody specifically binds to human CD4.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The SK3 / Anti-Leu 3a antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:
Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: SK3 / Anti-Leu 3a), Mouse IgG1 Kappa
Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: SK3 / Anti-Leu 3a), Human IgG1 Fc Silent Kappa
Background:
The recombinant SK3 / Anti-Leu 3a antibody binds to the human CD4 protein (cluster of differentiation 4), a glycoprotein and the co-receptor for the T-cell receptor (TCR). The CD4 protein is expressed on the surface of immune cells such as helper T cells, monocytes, macrophages, and dendritic cells.
CD4+ T-cell subsets in inflammatory diseases: beyond the Th1/Th2 paradigm
Hirahara, K., et al. (2016). Int Immunol. 2016 Apr;28(4):163-71. doi: 10.1093/intimm/dxw006. PMID: 26969728
The SK3 antibody (anti-human CD4, Leu 3a) was used in flow cytometry to identify CD4+ T-helper subsets in human peripheral blood mononuclear cells. SK3 staining enabled accurate gating of CD4+ cells, revealing distinct cytokine profiles in inflammatory conditions and highlighting novel therapeutic targets for autoimmune diseases.
Tags: activity of SK3 / Anti-Leu 3a antibody; function of SK3 / Anti-Leu 3a antibody
Flow cytometric analysis of CD4 expression in HIV patients using Anti-Leu 3a
Nicholson, J. K., et al. (1990). Clin Immunol Immunopathol. 1990 Sep;56(3):392-405. doi: 10.1016/0090-1229(90)90159-x. PMID: 2147101
The SK3 antibody (anti-human CD4, Leu 3a) was used in flow cytometry with a FACScan analyzer to quantify CD4+ T-cell counts in HIV-infected patients. SK3 staining correlated with disease progression, showing significant CD4+ cell reduction in advanced HIV, establishing reference ranges for clinical monitoring.
Tags: SK3 / Anti-Leu 3a antibody; SK3 / Anti-Leu 3a antibody for animal model
Comparison of Leu 3a and OKT4 in flow cytometry for T helper cell detection
Landay, A., et al. (1988). Blood. 1988 Feb;71(2):456-62. PMID: 3288135
The SK3 antibody (anti-human CD4, Leu 3a) was compared to OKT4 in flow cytometry for CD4+ T-cell detection in peripheral blood lymphocytes. SK3 showed higher sensitivity in low CD4+ populations, particularly in HIV patients, validating its use for clinical diagnostics over less consistent OKT4 staining.
Tags: SK3 / Anti-Leu 3a antibody for flow cytometry; SK3 / Anti-Leu 3a antibody for mouse tumor model
Flow cytometric determination of T lymphocyte subsets using Leu series antibodies
Hoffman, R. A., et al. (1985). Clin Immunol Immunopathol. 1985 Dec;37(3):337-47. doi: 10.1016/0090-1229(85)90104-7. PMID: 2417814
The SK3 antibody (anti-human CD4, Leu 3a) was used in flow cytometry with a Coulter EPICS-C cytometer to identify helper T cells in healthy donors. SK3 staining reliably distinguished CD4+ from CD8+ subsets, optimizing protocols for clinical quantification of T lymphocyte subsets.
Tags: SK3 / Anti-Leu 3a antibody mouse dose range; SK3 / Anti-Leu 3a mAb for animal model
Leu 3a antibody in flow cytometry for monitoring immune status in AIDS
Fauci, A. S., et al. (1985). N Engl J Med. 1985 Mar 28;312(13):831-8. doi: 10.1056/NEJM198503283121201. PMID: 2983206
The SK3 antibody (anti-human CD4, Leu 3a) was used in flow cytometry with a FACStar analyzer to monitor CD4+ T-cell counts in AIDS patients. SK3 staining confirmed CD4+ cell depletion as a hallmark of disease progression, establishing CD4 monitoring as a clinical standard.
Tags: SK3 / Anti-Leu 3a mAb for flow cytometry; SK3 / Anti-Leu 3a mAb for mouse tumor model
For more references about anti-human CD4 antibody (SK3 / Anti-Leu 3a), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-human CD4 monoclonal antibodies:
Clenoliximab biosimilar, research grade, anti-human CD4 monoclonal antibody
Ibalizumab biosimilar, research grade, anti-human CD4 monoclonal antibody
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4)
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A)
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2)
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a)
Syd Labs provides the following in vivo grade recombinant anti-mouse CD4 monoclonal antibodies:
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: GK1.5)
Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a) for flow cytometry
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