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Recombinant mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant mouse IgG1 Monoclonal Antibody.
Clone: 13B8.2.
Isotype: Mouse IgG1 kappa.
Source: The anti-human CD4 monoclonal antibody (clone: 13B8.2) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 13B8.2) specifically binds to human CD4.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD4 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Background
The recombinant 13B8.2 antibody binds to the human CD4 protein (cluster of differentiation 4), a glycoprotein and the co-receptor for the T-cell receptor (TCR). The CD4 protein is expressed on the surface of immune cells such as helper T cells, monocytes, macrophages, and dendritic cells.
Functionally specialized human CD4+ T-cell subsets express physicochemically distinct TCRs
Silvin, A., et al. (2020). Elife. 9:e57063. doi: 10.7554/eLife.57063. PMID: 33289628
Peripheral blood mononuclear cells (PBMCs) from 12 donors were stained with anti-CD4–PE (clone 13B8.2) antibody, along with anti-CD27–PE-Cy5 (clone O323), anti-CD25–eFluor450 (clone BC96), anti-CD31–PE-Cy7 (clone WM59), and anti-CD45RA–FITC (clone JS-83) to identify recent thymic emigrants (RTEs), mature naive T cells, and naive Tregs in the CD4+ lineage. The study explored the plasticity of Th2, Th1, Th17, and Treg cells, noting that Th2 cells can adopt a Th1-like phenotype, while Th1 and Th17 cells can transition to Th2 in helminth-infected mice, and Th17 cells can acquire regulatory features like IL-10 production under IL-12 or IL-27 influence.
Tags: anti-human CD4 13B8.2; anti-human CD4 13B8.2 antibody
Macrophage infection via selective capture of HIV-1-infected CD4+ T cells
Baxter, A. E., et al. (2014). Cell Host Microbe. 16(6):711-21. doi: 10.1016/j.chom.2014.10.010. PMID: 25467409
The study used the 13B8.2 antibody (anti-human CD4, 10 μg/ml) as an inhibitor of HIV-1 Env-receptor interactions, alongside soluble CD4, Tak-779, 2G12, and T20. The results suggest that macrophage uptake of HIV-1-infected CD4+ T cells contributes to the macrophage reservoir and viral dissemination in vivo, particularly after transmission or during massive CD4+ T cell death in lymphoid tissues. Detection of viral DNA or proteins in phagocytes may indicate uptake of infected immune cells rather than direct infection.
Tags: anti-human CD4 13B8.2 in vivo; anti-human CD4 13B8.2 in animal model
In vitro antitumoral activity of baculovirus-expressed chimeric recombinant anti-CD4 antibody 13B8.2 on T-cell lymphomas
Troadec, S., et al. (2007). J Immunother. 30(2):190-202. doi: 10.1097/01.cji.0000210069.83727.91. PMID: 17273799
A baculovirus-expressed chimeric recombinant IgG1 antibody (rIgG1) with human Cγ1 and Cκ domains, derived from the murine anti-CD4 monoclonal antibody 13B8.2, was tested for antitumoral activity against CD4+ T-cell lymphomas. The 13B8.2 antibody, specific for the CDR3-like loop of CD4, inhibited HIV-1 replication and T cell proliferation in vitro and showed promising results in CD4+ lymphoma cell lines, leading to plans for in vivo preclinical studies in a murine xenograft model.
Tags: anti-human CD4 13B8.2 mAb in animal model; anti-human CD4 13B8.2 in cancer research
Molecular and cellular analysis of human immunodeficiency virus-induced apoptosis in lymphoblastoid T-cell-line-expressing wild-type and mutated CD4 receptors
Guillerm, C., et al. (1998). J Virol. 72(10):8061-72. doi: 10.1128/JVI.72.10.8061-8072.1998. PMID: 9733846
The 13B8.2 monoclonal antibody, targeting the CD4 region critical for receptor dimerization, prevented apoptosis in HIV-infected cells without inhibiting viral replication. The study highlighted high levels of HIV replication and immune system activation in vivo, contributing to enhanced apoptosis in both infected and bystander CD4 cells. Fas signaling was noted as a potential contributor to T-cell depletion in HIV-1-infected patients.
Tags: bioactivity of anti-human CD4 13B8.2; anti-human CD4 13B8.2 of low endotoxin
Differential CD4-dependent inhibition of JNK but not Erk-2 activities by anti-CD4 monoclonal antibodies
Chene, C., et al. (1998). Int Immunol. 10(7):869-78. doi: 10.1093/intimm/10.7.869. PMID: 9701025
The study observed differential inhibition of JNK but not Erk-2 activities by the anti-CD4 monoclonal antibody 13B8.2 in Jurkat T cells. In vivo experiments in mouse models showed that administration of 13B8.2 led to selective inhibition of JNK activity in T cells, demonstrating that CD4 ligation modulates T cell activation through differential kinase inhibition.
Tags: anti-CD4 (13B8.2) monoclonal antibody; clone 13B8.2 of human CD4 antibody
For more references about anti-human CD4 antibody (13B8.2), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-human CD4 monoclonal antibodies:
Clenoliximab biosimilar, research grade, anti-human CD4 monoclonal antibody
Ibalizumab biosimilar, research grade, anti-human CD4 monoclonal antibody
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4)
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A)
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2)
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a)
Syd Labs provides the following in vivo grade recombinant anti-mouse CD4 monoclonal antibodies:
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: GK1.5)
Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a) for flow cytometry
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