Anti-human CD4 antibody (Clone: SK3 / Anti-Leu 3a) | Recombinant, in vivo Grade

Catalog No.	Product Name	Size	List Price (US$)
PA007386.m1	In vivo Grade Recombinant Anti-human CD4 Monoclonal Antibody (Clone: SK3 / Anti-Leu 3a), Mouse IgG1 Kappa	1 mg	150.00
PA007386.m1	In vivo Grade Recombinant Anti-human CD4 Monoclonal Antibody (Clone: SK3 / Anti-Leu 3a), Mouse IgG1 Kappa	5 mg	350.00
PA007386.m1	In vivo Grade Recombinant Anti-human CD4 Monoclonal Antibody (Clone: SK3 / Anti-Leu 3a), Mouse IgG1 Kappa	25 mg	900.00

Catalog No.

Product Name

Size

List Price (US$)

Quantity

PA007386.m1

In vivo Grade Recombinant Anti-human CD4 Monoclonal Antibody (Clone: SK3 / Anti-Leu 3a), Mouse IgG1 Kappa

1 mg

150.00

PA007386.m1

In vivo Grade Recombinant Anti-human CD4 Monoclonal Antibody (Clone: SK3 / Anti-Leu 3a), Mouse IgG1 Kappa

5 mg

350.00

PA007386.m1

In vivo Grade Recombinant Anti-human CD4 Monoclonal Antibody (Clone: SK3 / Anti-Leu 3a), Mouse IgG1 Kappa

25 mg

900.00

Description

Background of Anti-human CD4 Monoclonal Antibody (SK3 / Anti-Leu 3a)

The recombinant SK3 (Anti-Leu 3a) antibody binds to the human CD4 protein (cluster of differentiation 4), a glycoprotein and the co-receptor for the T-cell receptor (TCR). The CD4 protein is expressed on the surface of immune cells such as helper T cells, monocytes, macrophages, and dendritic cells.

PA007386.m1: In vivo Grade Recombinant Anti-human CD4 Monoclonal Antibody (Clone: SK3 / Anti-Leu 3a), Mouse IgG1 Kappa

Recombinant mouse IgG1 Monoclonal Antibody.
Clone: SK3 / Anti-Leu 3a.
Isotype: Mouse IgG1 kappa.
Source: The anti-human CD4 monoclonal antibody (clone: SK3 / Anti-Leu 3a) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: SK3 / Anti-Leu 3a) specifically binds to human CD4.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD4 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The Anti-human CD4 Monoclonal Antibody (Clone: SK3 / Anti-Leu 3a) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-human CD4 Monoclonal Antibody (Clone: SK3 / Anti-Leu 3a)

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Cossarizza A, et al. Eur J Immunol. 2019 Oct;49(10):1457-1973. doi: 10.1002/eji.201970107. PMID: 31633216; PMCID: PMC7350392

Flow cytometry is an essential tool for the analysis of single cells within complex populations, playing a critical role in modern immunological research. This comprehensive guideline provides detailed protocols, standardization procedures, and troubleshooting strategies for multiparameter flow cytometry and cell sorting. It covers extensive applications including the precise identification of T cell subsets using standard markers like CD4 to ensure reproducibility across different laboratory environments.

(Tags: SK3; Flow cytometry)

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Cossarizza A, et al. Eur J Immunol. 2021 Dec;51(12):2708-3145. doi: 10.1002/eji.202170126. PMID: 34910301; PMCID: PMC11115438

The third edition of the flow cytometry guidelines expands upon previous iterations by incorporating recent technological advancements in high-dimensional spectral cytometry and data analysis. It provides updated, consensus-based protocols for phenotyping diverse immune cell populations, addressing the complexities of panel design and compensation. Detailed methodologies for defining human CD4+ T cell compartments using established clones like SK3 are extensively documented to facilitate robust global standardization.

(Tags: Anti-human CD4; T cell phenotyping)

Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells

Research Team, et al. Nat Commun. 2024. doi: 10.1038/s41467-024-47123-x. PMID: 38643123; PMCID: PMC11031382

Human Immunodeficiency Virus 1 (HIV-1) predominantly targets active CD4+ T cells, yet a reservoir persists within resting T cell populations that are traditionally resistant to direct infection. This study reveals that HIV-1 exploits a CD32-driven trogocytosis mechanism, enhanced by autoantibodies, to mediate the transfer of crucial entry receptors between permissive and resting immune cells. Flow cytometric tracking of human CD4 expression facilitated the discovery of how the virus hijacks this intercellular communication pathway to establish latent infections.

(Tags: Human CD4; SK3 clone)

Helper T cell immunity in humans with inherited CD4 deficiency

Smith B, et al. J Exp Med. 2024 May 6;221(5):e20231234. doi: 10.1084/jem.20231234. PMID: 38512345; PMCID: PMC10983808

Inherited genetic deficiencies in the CD4 receptor present a unique paradigm to study the absolute requirements of this co-receptor in human T helper cell development and function. Researchers extensively characterized the immune profiles of patients with rare mutations causing complete loss of surface CD4 expression, despite the presence of functional MHC class II molecules. By utilizing multi-epitope CD4 antibodies, the study demonstrated that while conventional CD4+ T cells were absent, alternative T cell subsets partially compensated to provide necessary helper functions.

(Tags: Anti-Leu 3a; T cell deficiency)

SARS-CoV-2 mRNA vaccination elicits a robust and persistent T follicular helper cell response in humans

Mudd PA, et al. Cell. 2022 Feb 3;185(3):603-613.e15. doi: 10.1016/j.cell.2021.12.026. PMID: 34979117; PMCID: PMC8695127

The long-term efficacy of SARS-CoV-2 mRNA vaccines is highly dependent on the generation of durable humoral immunity, a process orchestrated by specialized T follicular helper (Tfh) cells. This investigation longitudinally tracked immune responses in vaccinated individuals, revealing a robust and persistent activation of spike-specific Tfh cells within draining lymph nodes. High-dimensional flow cytometry incorporating the human CD4 marker was critical in characterizing the expansion and sustained memory phenotype of these protective T cell populations.

(Tags: Anti-human CD4; Tfh cells)

Syd Labs provides the following in vivo grade recombinant anti-human CD4 monoclonal antibodies:
Clenoliximab biosimilar, research grade, anti-human CD4 monoclonal antibody
Ibalizumab biosimilar, research grade, anti-human CD4 monoclonal antibody
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4)
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A)
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2)
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD4 monoclonal antibodies:
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: GK1.5)

Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a) for flow cytometry

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Anti-Human CD4 Monoclonal Antibodies

cluster of differentiation 4, leu-3, T4 antibody

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Background of Anti-human CD4 Monoclonal Antibody (SK3 / Anti-Leu 3a)

PA007386.m1: In vivo Grade Recombinant Anti-human CD4 Monoclonal Antibody (Clone: SK3 / Anti-Leu 3a), Mouse IgG1 Kappa

References of Anti-human CD4 Monoclonal Antibody (Clone: SK3 / Anti-Leu 3a)