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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
|---|
Recombinant mouse IgG2b isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2b Monoclonal Antibody.
Clone: OKT4.
Isotype: Mouse IgG2b kappa.
Source: The anti-human CD4 monoclonal antibody (clone: OKT4) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant OKT4 antibody specifically binds to human CD4.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: OKT4.
Isotype: Human IgG1 kappa.
Source: The anti-human CD4 monoclonal antibody (clone: OKT4) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant OKT4 antibody specifically binds to human CD4.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The OKT4 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:
Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4), Mouse IgG2b Kappa
Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4), Human IgG1 Fc Silent Kappa
Background:
The recombinant OKT4 antibody binds to the human CD4 protein (cluster of differentiation 4), a glycoprotein and the co-receptor for the T-cell receptor (TCR). The CD4 protein is expressed on the surface of immune cells such as helper T cells, monocytes, macrophages, and dendritic cells.
Single amino acid substitution in the V3 domain of CD4 is responsible for OKT4 epitope deficiency
Kawamura, N., et al. (1991). Immunogenetics. 1991;34(3):208-10. doi: 10.1007/BF00205826. PMID: 1716607
The OKT4 antibody (anti-human CD4) was used to identify a deficiency in the CD4 epitope in patients with normal CD4+ T cell counts, as detected by other anti-CD4 monoclonal antibodies. This study linked a single amino acid substitution in the V3 domain of CD4 to the OKT4 epitope deficiency, relevant to understanding HIV pathogenesis as CD4 is the HIV receptor.
Tags: activity of OKT4 antibody; function of OKT4 antibody
Surface antigen changes occurring in short-term cultures of activated human T lymphocytes: analysis by flow cytometry
Boylston, A. W., et al. (1982). Cell Immunol. 1982 Jul 15;71(1):12-26. doi: 10.1016/0008-8749(82)90492-0. PMID: 6183008
The OKT4 antibody (anti-human CD4) was used in flow cytometry to analyze surface antigen changes in activated T lymphocytes. The study observed a shift in the OKT4:OKT8 ratio from 60:40 to 40:60 in cultured T lymphoblasts, suggesting a possible phenotype change from OKT4+ to OKT8+ cells, while OKT4 expression remained stable.
Tags: OKT4 antibody; OKT4 antibody for animal model
Reversible alterations in immunoregulatory T cells in smoking. Analysis by monoclonal antibodies and flow cytometry
Ferson, M., et al. (1982). Chest. 1982 Nov;82(5):526-9. doi: 10.1378/chest.82.5.526. PMID: 6982152
The OKT4 antibody (anti-human CD4) was used in flow cytometry to assess T-cell subsets in heavy smokers, revealing a decreased percentage of OKT4+ cells and a reduced OKT4+/OKT8+ ratio. These changes normalized six weeks after smoking cessation, indicating reversible alterations in immunoregulatory T cells linked to smoking.
Tags: OKT4 antibody for flow cytometry; OKT4 antibody for mouse tumor model
Quantitative immunofluorescent assay of full-length, recombinant CD4 in solution and mapping of its epitopes
Lyding, J., et al. (1991). J Fluoresc. 1991 Jun;1(2):141-6. doi: 10.1007/BF00865210. PMID: 24242964
The OKT4 antibody (anti-human CD4) was used in a quantitative flow cytometry assay with Simply Cellular microbeads to measure recombinant CD4 antigen levels. The assay, using standard conditions for OKT4 binding and detection, enabled epitope mapping and determination of CD4 oligomerization levels in solution.
Tags: OKT4 antibody mouse dose range; OKT4 mAb for animal model
Asbestos exposure correlates with alterations in circulating T cell subsets
de Shazo, R. D., et al. (1985). Am J Med Sci. 1985 Jan;289(1):1-5. doi: 10.1097/00000441-198501000-00001. PMID: 3929128
The OKT4 antibody (anti-human CD4) was used in flow cytometry to assess alterations in circulating T-cell subsets in asbestos-exposed individuals. The study identified changes in CD4+ T-cell populations, suggesting microbial metabolites like ascorbate influence CD4+ T-cell function, with implications for Th17-mediated conditions like Crohn’s disease.
Tags: OKT4 mAb for flow cytometry; OKT4 monoclonal antibody
For more references about anti-human CD4 antibody (OKT4), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-human CD4 monoclonal antibodies:
Clenoliximab biosimilar, research grade, anti-human CD4 monoclonal antibody
Ibalizumab biosimilar, research grade, anti-human CD4 monoclonal antibody
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4)
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A)
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2)
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a)
Syd Labs provides the following in vivo grade recombinant anti-mouse CD4 monoclonal antibodies:
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: GK1.5)
Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a) for flow cytometry
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