Anti-Human CD4 (13B8.2) | Recombinant, Flow Cytometry - Syd Labs

Catalog No.	Product Name	Size	List Price (US$)
PA007415.m1	Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Mouse IgG1 Kappa	50 ug	100.00
PA007415.m1	Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Mouse IgG1 Kappa	200 ug	200.00
PA007415.h1Fs	Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Human IgG1 Fc Silent Kappa	50 ug	200.00
PA007415.h1Fs	Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Human IgG1 Fc Silent Kappa	200 ug	400.00
PA007758.h1Fs-b	Biotinylated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Human IgG1 Fc Silent Kappa	50 ug	150.00
PA007758.h1Fs-b	Biotinylated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Human IgG1 Fc Silent Kappa	200 ug	300.00

Catalog No.

Product Name

Size

List Price (US$)

Quantity

PA007415.m1

Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Mouse IgG1 Kappa

50 ug

100.00

PA007415.m1

Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Mouse IgG1 Kappa

200 ug

200.00

PA007415.h1Fs

Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Human IgG1 Fc Silent Kappa

50 ug

200.00

PA007415.h1Fs

Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Human IgG1 Fc Silent Kappa

200 ug

400.00

PA007758.h1Fs-b

Biotinylated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Human IgG1 Fc Silent Kappa

50 ug

150.00

PA007758.h1Fs-b

Biotinylated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Human IgG1 Fc Silent Kappa

200 ug

300.00

Description

PA007415.m1: Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Mouse IgG1 Kappa

Recombinant Mouse IgG1 Monoclonal Antibody.
Clone: 13B8.2.
Isotype: Mouse IgG1 kappa.
Source: The anti-human CD4 monoclonal antibody (clone: 13B8.2) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 13B8.2 antibody specifically binds to human CD4.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007415.h1Fs: Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Human IgG1 Fc Silent Kappa

Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: 13B8.2.
Isotype: Human IgG1 kappa.
Source: The anti-human CD4 monoclonal antibody (clone: 13B8.2) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 13B8.2 antibody specifically binds to human CD4.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007758.h1Fs-b: Biotinylated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: 13B8.2.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated anti-human CD4 monoclonal antibody (clone: 13B8.2) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant 13B8.2 antibody specifically binds to human CD4.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The 13B8.2 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

Background:

The recombinant 13B8.2 antibody binds to the human CD4 protein (cluster of differentiation 4), a glycoprotein and the co-receptor for the T-cell receptor (TCR). The CD4 protein is expressed on the surface of immune cells such as helper T cells, monocytes, macrophages, and dendritic cells.

References of Anti-Human CD4 Monoclonal Antibody (Clone: 13B8.2)

In vitro antitumoral activity of baculovirus-expressed chimeric recombinant anti-CD4 antibody 13B8.2 on T-cell lymphomas

Troadec, S., et al. J Immunother. 2007 Feb-Mar;30(2):190-202. PMID: 17273799

The chimeric recombinant anti-CD4 antibody 13B8.2, expressed in baculovirus, was tested for antitumoral activity against T-cell lymphomas using flow cytometry. The 13B8.2 antibody induced apoptosis in CD4+ Jurkat cells, as confirmed by Annexin V staining, and significantly reduced CD4+ T cells in treated lymphoma cell lines. Cell viability was assessed after 48 hours using MTT assays, and the efficacy of 13B8.2 was compared with other anti-CD4 antibodies in vitro.

(Tags: activity of 13B8.2 antibody; function of 13B8.2 antibody)

An anti-CD4 (CDR3-loop) monoclonal antibody inhibits human immunodeficiency virus type 1 envelope glycoprotein-induced apoptosis

Guillerm, C., et al. Virology. 1998 Sep 15;248(2):254-63. PMID: 9743188

The anti-CD4 monoclonal antibody 13B8.2, targeting the CDR3-like region of CD4, was used to assess HIV-1 envelope glycoprotein-induced apoptosis via flow cytometry. Preincubation with 13B8.2 significantly reduced apoptosis in HIV-1-infected T cells and blocked CD4 crosslinking by gp120. The study also demonstrated that 13B8.2 inhibits syncytium formation in vitro, with cell surface CD4 expression quantified using fluorescently labeled antibodies.

(Tags: 13B8.2 antibody; 13B8.2 antibody for animal model)

The Cytoplasmic Tail of CD4 Is Required for Inhibition of HIV-1 Env-Mediated Cell Fusion

Albright, C. A., et al. J Virol. 1997 Jun;71(6):4686-91. PMID: 9171443

The 13B8.2 antibody (anti-human CD4 antibody) was used to detect CD4 expression on transfected HeLa cells by flow cytometry to study HIV-1 envelope-mediated cell fusion. Mutations in the CD4 cytoplasmic tail abolished the inhibitory effect on cell fusion, while flow cytometry confirmed that CD4 surface expression remained unchanged. HIV-1 envelope glycoprotein binding was assessed through co-culture experiments.

(Tags: 13B8.2 antibody for cancer research; 13B8.2 antibody for flow cytometry)

CD4 is required for inhibition of human immunodeficiency virus type 1 replication by antibodies that bind to the immunoglobulin CDR3-like region in domain 1 of CD4

Benkirane, M., et al. J Virol. 1995 Nov;69(11):6904-10. PMID: 7568058

The anti-CD4 antibody 13B8.2, targeting the immunoglobulin CDR3-like region, was used to inhibit HIV-1 replication in Jurkat cells, with CD4 expression monitored by flow cytometry. The 13B8.2 antibody effectively blocked HIV-1 provirus transcription without significantly altering CD4 surface levels. Inhibition of HIV-1 entry was confirmed using syncytium formation assays.

(Tags: 13B8.2 antibody mouse dose range; 13B8.2 mAb for cancer research)

An antibody that binds the immunoglobulin CDR3-like region of the CD4 molecule inhibits provirus transcription in HIV-infected T cells

Benkirane, M., et al. EMBO J. 1993 Dec;12(12):4909-21. PMID: 8102412

The 13B8.2 antibody (anti-human CD4 mAb), targeting the immunoglobulin CDR3-like region of CD4, was used to inhibit provirus transcription in HIV-1-infected Jurkat cells, with CD4 expression quantified by flow cytometry. The study showed that 13B8.2 does not affect HIV-1 entry but targets post-entry events, with stable CD4 expression confirmed during treatment.

(Tags: 13B8.2 mAb for mouse tumor model; 13B8.2 monoclonal antibody)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD4 monoclonal antibodies:
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: GK1.5)
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: YTS191)
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: YTS 177.9.6.1)
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: YTA 3.1.2)

Syd Labs provides the following in vivo grade recombinant anti-human CD4 monoclonal antibodies:
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-Leu 3a)
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4)
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2)
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A)
Clenoliximab biosimilar, research grade, anti-human CD4 monoclonal antibody
Ibalizumab biosimilar, research grade, anti-human CD4 monoclonal antibody

Syd Labs provides the following recombinant anti-mouse CD4 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: GK1.5) for flow cytometry

Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-Leu 3a) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A) for flow cytometry

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Anti-Human CD4 Monoclonal Antibodies for Flow Cytometry

cluster of differentiation 4, leu-3, T4

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PA007415.m1: Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Mouse IgG1 Kappa

PA007415.h1Fs: Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Human IgG1 Fc Silent Kappa

PA007758.h1Fs-b: Biotinylated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Human IgG1 Fc Silent Kappa

References of Anti-Human CD4 Monoclonal Antibody (Clone: 13B8.2)