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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG1 isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG1 Monoclonal Antibody.
Clone: 13B8.2.
Isotype: Mouse IgG1 kappa.
Source: The anti-human CD4 monoclonal antibody (clone: 13B8.2) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 13B8.2 antibody specifically binds to human CD4.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: 13B8.2.
Isotype: Human IgG1 kappa.
Source: The anti-human CD4 monoclonal antibody (clone: 13B8.2) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 13B8.2 antibody specifically binds to human CD4.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The 13B8.2 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:
Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Mouse IgG1 Kappa
Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Human IgG1 Fc Silent Kappa
Background:
The recombinant 13B8.2 antibody binds to the human CD4 protein (cluster of differentiation 4), a glycoprotein and the co-receptor for the T-cell receptor (TCR). The CD4 protein is expressed on the surface of immune cells such as helper T cells, monocytes, macrophages, and dendritic cells.
In vitro antitumoral activity of baculovirus-expressed chimeric recombinant anti-CD4 antibody 13B8.2 on T-cell lymphomas
Troadec, S., et al. (2007). J Immunother. 30(2):190-202. doi: 10.1097/01.cji.0000210069.83727.91. PMID: 17273799
The chimeric recombinant anti-CD4 antibody 13B8.2, expressed in baculovirus, was tested for antitumoral activity against T-cell lymphomas using flow cytometry. The 13B8.2 antibody induced apoptosis in CD4+ Jurkat cells, as confirmed by Annexin V staining, and significantly reduced CD4+ T cells in treated lymphoma cell lines. Cell viability was assessed after 48 hours using MTT assays, and the efficacy of 13B8.2 was compared with other anti-CD4 antibodies in vitro.
Tags: activity of 13B8.2 antibody; function of 13B8.2 antibody
An anti-CD4 (CDR3-loop) monoclonal antibody inhibits human immunodeficiency virus type 1 envelope glycoprotein-induced apoptosis
Guillerm, C., et al. (1998). Virology. 248(2):254-63. doi: 10.1006/viro.1998.9265. PMID: 9743188
The anti-CD4 monoclonal antibody 13B8.2, targeting the CDR3-like region of CD4, was used to assess HIV-1 envelope glycoprotein-induced apoptosis via flow cytometry. Preincubation with 13B8.2 significantly reduced apoptosis in HIV-1-infected T cells and blocked CD4 crosslinking by gp120. The study also demonstrated that 13B8.2 inhibits syncytium formation in vitro, with cell surface CD4 expression quantified using fluorescently labeled antibodies.
Tags: 13B8.2 antibody; 13B8.2 antibody for animal model
The Cytoplasmic Tail of CD4 Is Required for Inhibition of HIV-1 Env-Mediated Cell Fusion
Albright, C. A., et al. (1997). J Virol. 71(6):4686-91. doi: 10.1128/JVI.71.6.4686-4691.1997. PMID: 9171443
The 13B8.2 antibody (anti-human CD4 antibody) was used to detect CD4 expression on transfected HeLa cells by flow cytometry to study HIV-1 envelope-mediated cell fusion. Mutations in the CD4 cytoplasmic tail abolished the inhibitory effect on cell fusion, while flow cytometry confirmed that CD4 surface expression remained unchanged. HIV-1 envelope glycoprotein binding was assessed through co-culture experiments.
Tags: 13B8.2 antibody for cancer research; 13B8.2 antibody for flow cytometry
CD4 is required for inhibition of human immunodeficiency virus type 1 replication by antibodies that bind to the immunoglobulin CDR3-like region in domain 1 of CD4
Benkirane, M., et al. (1995). J Virol. 69(11):6904-10. doi: 10.1128/JVI.69.11.6904-6910.1995. PMID: 7568058
The anti-CD4 antibody 13B8.2, targeting the immunoglobulin CDR3-like region, was used to inhibit HIV-1 replication in Jurkat cells, with CD4 expression monitored by flow cytometry. The 13B8.2 antibody effectively blocked HIV-1 provirus transcription without significantly altering CD4 surface levels. Inhibition of HIV-1 entry was confirmed using syncytium formation assays.
Tags: 13B8.2 antibody mouse dose range; 13B8.2 mAb for cancer research
An antibody that binds the immunoglobulin CDR3-like region of the CD4 molecule inhibits provirus transcription in HIV-infected T cells
Benkirane, M., et al. (1993). EMBO J. 12(12):4909-21. doi: 10.1002/j.1460-2075.1993.tb06185.x. PMID: 8102412
The 13B8.2 antibody (anti-human CD4 mAb), targeting the immunoglobulin CDR3-like region of CD4, was used to inhibit provirus transcription in HIV-1-infected Jurkat cells, with CD4 expression quantified by flow cytometry. The study showed that 13B8.2 does not affect HIV-1 entry but targets post-entry events, with stable CD4 expression confirmed during treatment.
Tags: 13B8.2 mAb for mouse tumor model; 13B8.2 monoclonal antibody
For more references about anti-human CD4 antibody (13B8.2), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-human CD4 monoclonal antibodies:
Clenoliximab biosimilar, research grade, anti-human CD4 monoclonal antibody
Ibalizumab biosimilar, research grade, anti-human CD4 monoclonal antibody
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4)
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A)
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2)
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a)
Syd Labs provides the following in vivo grade recombinant anti-mouse CD4 monoclonal antibodies:
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: GK1.5)
Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a) for flow cytometry
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