PA007625.r2b: In Vivo Grade Recombinant Anti-mouse CD4 Monoclonal Antibody (Clone: YTS191), Rat IgG2b Kappa
Recombinant rat IgG2b Monoclonal Antibody.
Clone: YTS191.
Isotype: Rat IgG2b kappa.
Source: The anti-mouse CD4 monoclonal antibody (clone: YTS191) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: YTS191) specifically binds to mouse CD4.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD4 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD4 monoclonal antibody of clone YTS191 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
References of anti-mouse CD4 antibody (YTS191)
NK/ILC1 cells mediate neuroinflammation and brain pathology following congenital CMV infection
Daria Kveštak, et al. (2021). J Exp Med. 2021 May 3;218(5):e20201503. doi: 10.1084/jem.20201503. PMID: 33630019
CD4 T cells were depleted in vivo by administration of 50 μg anti-CD4 antibody (YTS191.1.2) to MCMV-infected animals on days 4 and 7 p.i. ... For neutralization of IFN-γ, 50 μg anti–IFN-γ antibody (XMG1.2) was injected to MCMV-infected animals on days 1, 2, 4, and 6 p.i. ... CXCR3 was blocked in vivo by administration of anti-CXCR3 antibodies (CD183) to MCMV-infected animals on days 1, 3, 5, and 7 p.i. ... NK cells in C57BL/6 mice were depleted in vivo by administration of 50 μg anti-NK1.1 antibody (PK136) to MCMV-infected animals on days 1, 4, and 7 p.i. ... To determine whether NKp46+ cells have an early role in neuroinflammation and the pathogenesis of perinatal MCMV infection of the brain, we treated MCMV-infected BALB/c mice with anti-AGM1, which depletes brain-infiltrating NK and ILC1 cells, even though NK cells were depleted more efficiently.
Tags: activity of anti-mouse CD4 antibody; activity of YTS191 mAb
Critical roles for LIGHT and its receptors in generating T cell-mediated immunity during Leishmania donovani infection
Amanda C Stanley, et al. (2011). PLoS Pathog. 2011 Oct;7(10):e1002279. doi: 10.1371/journal.ppat.1002279. PMID: 21998581
Mice were administered 100 µg of anti-LTβR mAb (LLTB2) or anti-HVEM mAb (LH1) i.v. on the day of infection and every 5 days thereafter for 14 day experiments, or as a single dose on the day of infection for 7 day experiments... The anti-LTβR mAb 3C8 was administered at 200 µg i.v. starting at the times indicated in the text and every 5 days thereafter... Mice were depleted of CD4+ or CD8+ T cells with anti-CD4 (YTS191.1) or anti-CD8β (53-5.8) mAbs, respectively, as previously described... Depletion of T cell subsets was confirmed at completion of experiments by assessing T cell numbers in the spleen by flow cytometry... C57BL/6 mice and B6.Jα18−/− mice were treated with control rat IgG or anti-LTβR mAb on the day of L. donovani infection, with or without CD4+ or CD8+ T cell depletion, as indicated.
Tags: function of YTS191 mAb; anti-CD4 clone YTS191
CD4+ natural regulatory T cells prevent experimental cerebral malaria via CTLA-4 when expanded in vivo
Ashraful Haque, et al. (2010). PLoS Pathog. 2010 Dec 9;6(12):e1001221. doi: 10.1371/journal.ppat.1001221. PMID: 21170302
Anti-IL-10R (1B1.3a), anti-CD4 (YTS191), anti-IL-2 (S4B6 and JES6-1A12), anti-NK1.1 (PK136), and isotype control mAb (MAC49; ratIgG1) were purified from culture supernatants by protein G column purification (Amersham, Uppsala, Sweden) followed by endotoxin removal... Four days later, spleens were isolated, Foxp3+ CD4+ T cells were enumerated by flow cytometry, and IL-10 and IFNγ production by these cells was assessed directly ex vivo by intracellular cytokine staining. ... C) Four days after infection with PbA, splenic CD4+ Foxp3+ T cells from IL-2Jc treated and control saline treated mice we assessed for expression of CD25, Foxp3 and CTLA-4 by flow cytometry. ... Both antibody blockade treatments restored the splenic IFNγ CD4+ T cell response that had been impaired by IL-2Jc treatment (Figure 8C). ... Since we could detect only a modest role for IL-10 in IL-2Jc mediated protection of wild-type C57BL/6 mice, we further examined the effect of IL-2Jc treatment in IL-10−/− mice, and found that these animals were significantly protected against ECM in a CTLA-4-dependent manner (Figure S4).
Tags: YTS191 mAb in vivo; clone YTS191 of mouse CD4 antibody
For more references about anti-mouse CD4 antibody (YTS191), please contact our scientific support team with message@sydlabs.com.