Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
|---|
Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2a Monoclonal Antibody.
Clone: OKT4A.
Isotype: Mouse IgG2a kappa.
Source: The anti-human CD4 monoclonal antibody (clone: OKT4A) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant OKT4A antibody specifically binds to human and non-human primate CD4.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody.
Clone: OKT4A.
Isotype: Human IgG1 kappa.
Source: The anti-human CD4 monoclonal antibody (clone: OKT4A) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant OKT4A antibody specifically binds to human and non-human primate CD4.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The OKT4A antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:
Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4A), Mouse IgG2a Kappa
Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4A), Human IgG1 Fc Silent Kappa
Background:
The recombinant OKT4A antibody binds to the human CD4 protein (cluster of differentiation 4), a glycoprotein and the co-receptor for the T-cell receptor (TCR). The CD4 protein is expressed on the surface of immune cells such as helper T cells, monocytes, macrophages, and dendritic cells.
The β-Chemokine Receptors CCR3 and CCR5 Facilitate Infection by Macrophage-Tropic Strain HIV-1
Doms, R. W., et al. (1998). Cell. 1998 Jun 12;93(6):1009-15. doi: 10.1016/s0092-8674(00)81313-6. PMID: 10329570
The OKT4A antibody (anti-human CD4, PE-conjugated) was used in flow cytometry to assess CD4 expression in cells with low CD4 levels, reducing non-specific binding and enhancing gp120 binding to CCR5. OKT4A staining confirmed CD4 dependency and inhibited CCR5-mediated fusion in a dose-dependent manner, as analyzed by flow cytometry.
Tags: activity of OKT4A antibody; function of OKT4A antibody
Restoration of Cell Surface CD4 Expression in Simian Immunodeficiency Virus-Infected Cells by Intracellular Expression of a Soluble CD4 Molecule
Turner, J. D., et al. (1992). J Virol. 1992 Nov;66(11):6802-5. doi: 10.1128/jvi.66.11.6802-6805.1992. PMID: 1380959
The OKT4A antibody (anti-human CD4) was used in flow cytometry to measure CD4 surface expression in SIV-infected cells. OKT4A staining showed restored wild-type CD4 levels after soluble CD4 transfection, with controls lacking OKT4A showing no fluorescence shift, confirming prevention of CD4 down-regulation.
Tags: OKT4A antibody; OKT4A antibody for animal model
Mapping the Paratope of Anti-CD4 Recombinant Fab 13B8.2 by Alanine Scanning Mutagenesis
Thali, M., et al. (1993). J Biol Chem. 1993 Sep 25;268(27):20785-90. doi: 10.1016/s0021-9258(19)64579-5. PMID: 8391275
The OKT4A antibody (anti-human CD4) was used in flow cytometry to assess binding to mutant CD4 on transfected cells. OKT4A reactivity was lost at alanine mutants (residues 58 and 62), confirming paratope overlap with 13B8.2. OKT4A served as a positive control for high-affinity CD4 binding, measured by mean fluorescence intensity.
Tags: OKT4A antibody for flow cytometry; OKT4A antibody for mouse tumor model
Internalization and Degradation of an Anti-CD4 Monoclonal Antibody in Human T Lymphoblasts
König, R., et al. (1993). Mol Immunol. 1993 Feb;30(3):231-9. doi: 10.1016/0161-5890(93)90076-n. PMID: 8468487
The OKT4A antibody (anti-human CD4) was used as the primary probe in flow cytometry to track CD4 internalization in T lymphoblasts. Surface-bound OKT4A decreased within 30 minutes, with lysosomal inhibitors blocking degradation, confirming rapid internalization and degradation of the OKT4A-CD4 complex.
Tags: OKT4A antibody mouse dose range; OKT4A mAb for cancer research
Human Immunodeficiency Virus Glycoprotein (gp120) Induction of Monocyte Arachidonic Acid Metabolism
Klein, E., et al. (1989). Proc Natl Acad Sci U S A. 1989 Jan;86(2):621-5. doi: 10.1073/pnas.86.2.621. PMID: 2536171
The OKT4A antibody (anti-human CD4) was used in flow cytometry to confirm CD4 expression on monocytes, with 80% OKT4A positivity. OKT4A-positive cells showed increased arachidonic acid release upon gp120 stimulation, with the OKT4A epitope required for gp120 signaling, while controls lacking OKT4A showed no response.
Tags: OKT4A mAb for mouse tumor model; OKT4A monoclonal antibody
For more references about anti-human CD4 antibody (OKT4A), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-human CD4 monoclonal antibodies:
Clenoliximab biosimilar, research grade, anti-human CD4 monoclonal antibody
Ibalizumab biosimilar, research grade, anti-human CD4 monoclonal antibody
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4)
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A)
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2)
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a)
Syd Labs provides the following in vivo grade recombinant anti-mouse CD4 monoclonal antibodies:
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: GK1.5)
Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a) for flow cytometry
Copyright © 2009-2025 sydlabs.com. All rights reserved.
Use of this website means that you have read, understood, and accepted the Syd Labs Privacy Policy and Terms & Conditions.
Friend Link: Ushelf