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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Background
Recombinant mouse anti-biotin antibodies recognize biotin (vitamin B7) attached to proteins, peptides, nucleic acids, or solid matrices, and can be offered in fluorophore and enzyme-conjugated versions.
The anti-biotin monoclonal antibody can be used as a second-step reagent in various applications for detecting biotinylated targets. The biotin-streptavidin system is widely used in immunoassay signal amplification techniques because streptavidin binds biotin with extremely high affinity.
Because biotin is naturally present in a large variety of biological samples, it may affect the results of biotin-based assays, such as reduced sensitivity, false negatives or false positives. Streptavidin/biotin blocking kit, avidin/biotin blocking system, or biotin blockers are used to block signals from endogenous avidin, biotin and biotin-binding proteins in tissues when used with biotin-based IHC detection.
Please contact us to ask for a quote for the engineered anti-biotin antibody mutants of various isotypes, and mutants with Avi-, His-, and DYKDDDDK-tags, and biotinylated antibodies and mutants. A variety of conjugates (such as dyes and fluorophores) with the anti-biotin antibodies and mutants are available.
Recombinant human IgG1 Monoclonal Antibody.
Clone: F1.
Isotype: Human IgG1 kappa.
Source: The recombinant anti-Biotin monoclonal antibody (clone: F1) was produced in mammalian cells.
Specificity/Sensitivity: The recombinant anti-Biotin monoclonal antibody (clone: F1) specifically binds to Biotin.
Applications: ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)).
Form of Antibody: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Recombinant human IgG1-I253A H310A H435A (IAHAHA) Monoclonal Antibody.
Clone: F1.
Isotype: Human IgG1-I253A H310A H435A (IAHAHA) Kappa.
Source: The recombinant anti-Biotin monoclonal antibody (clone: F1) was produced in mammalian cells.
Specificity/Sensitivity: The recombinant anti-Biotin monoclonal antibody (clone: F1) specifically binds to Biotin.
Applications: ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)).
Form of Antibody: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The recombinant anti-Biotin monoclonal antibody of clone F1 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Syd Labs provides the following in vivo grade recombinant anti-Biotin antibodies:
In Vivo Grade Recombinant Anti-Biotin Antibodies (Clone F1)
Syd Labs provides the following recombinant anti-Fluorescein/Digoxin/Biotin/Phospho-Tyrosine monoclonal antibodies:
Recombinant Anti-Fluorescein Monoclonal Antibodies (Clone 4-4-20)
Recombinant Anti-Fluorescein Monoclonal Antibodies (Clone 4M5.3)
Recombinant Anti-Digoxin Monoclonal Antibodies (Clone 5A7)
Recombinant Anti-Digoxin Monoclonal Antibodies (Clone 26-10)
Recombinant Anti-Biotin Monoclonal Antibodies (Clone M33)
Recombinant Anti-Biotin Monoclonal Antibodies (Clone F1)
Recombinant Anti-Phospho-Tyrosine Monoclonal Antibodies (Clone Py20)
Recombinant Anti-Phospho-Tyrosine Monoclonal Antibodies (Clone 4G10)
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