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HA-Tag Antibody

Anti-HA-Tag Antibody, HA-Tag Antibody (12CA5), Clone 12CA5, YPYDVPDYA epitope

Catalog No. Product Name Size List Price (US$) Quantity
PA007281.m2b Recombinant Anti-HA-Tag Monoclonal Antibody (clone 12CA5), Mouse IgG2b Kappa 50 ug 100.00
PA007281.m2b Recombinant Anti-HA-Tag Monoclonal Antibody (clone 12CA5), Mouse IgG2b Kappa 100 ug 150.00
PA007281.m2b Recombinant Anti-HA-Tag Monoclonal Antibody (clone 12CA5), Mouse IgG2b Kappa 200 ug 275.00
PA007281.m2b Recombinant Anti-HA-Tag Monoclonal Antibody (clone 12CA5), Mouse IgG2b Kappa 1 mg 500.00
PA007281.m2b Recombinant Anti-HA-Tag Monoclonal Antibody (clone 12CA5), Mouse IgG2b Kappa 5 mg 1500.00
PA007281.mf Recombinant Anti-HA-Tag Mouse Fab Fragment (Clone 12CA5) 1 mg 900.00
PA007281.scFv Recombinant Anti-HA-Tag scFv (clone 12CA5), His Tag 1 mg 900.00
PA007281.h1 Recombinant Anti-HA-Tag Monoclonal Antibody (Clone 12CA5),Human lgG1 Kappa 1 mg 500.00
PA007281.h1 Recombinant Anti-HA-Tag Monoclonal Antibody (Clone 12CA5),Human lgG1 Kappa 5 mg 1500.00
PA007281.rt Recombinant Anti-HA-Tag Monoclonal Antibody (Clone 12CA5), Rabbit IgG 1 mg 500.00
Description

PA007281.m2b: Recombinant Anti-HA-Tag Monoclonal Antibody (clone 12CA5), Mouse IgG2b Kappa

The recombinant anti-HA-Tag monoclonal antibody (clone 12CA5), mouse IgG2b kappa, is produced in mammalian cells. It consists of mouse variable regions and mouse IgG2b kappa constant regions.
Clone: 12CA5.
Isotype: mouse IgG2b kappa.
Specificity/Sensitivity: the 9-amino acid sequence YPYDVPDYA, derived from influenza virus hemagglutinin (HA) on HA tagged proteins and HA tag expression cells.
Applications: ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls. 
Reactivity: Human, Mouse, Rat, Others.
Purification: The HA-tag antibody was affinity-purified from supernatant of mammalian cells cultured in a chemically defined medium by affinity-chromatography using protein A.
Form of Antibody: 1 mg/ml in 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007281.h1: Recombinant Anti-HA-Tag Monoclonal Antibody (clone 12CA5), Human IgG1 Kappa

The recombinant anti-HA-Tag monoclonal antibody (clone 12CA5), human IgG1 kappa, is produced in mammalian cells. It consists of mouse variable regions and human IgG1 kappa constant regions.
Clone: 12CA5.
Isotype: human IgG1 Kappa.
Specificity/Sensitivity: the 9-amino acid sequence YPYDVPDYA, derived from influenza virus hemagglutinin (HA) on HA tagged proteins and HA tag expression cells.
Applications: ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls. 
Reactivity: Human, Mouse, Rat, Others.
Purification: The HA-tag antibody was affinity-purified from supernatant of mammalian cells cultured in a chemically defined medium by affinity-chromatography using protein A.
Form of Antibody: 1 mg/ml in 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The recombinant anti-HA-Tag monoclonal antibody (clone 12CA5) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap.
1 month from date of receipt, 2 to 8°C as supplied.
12 months from date of receipt, -20°C to -70°C as supplied.

References of Anti-HA-Tag Monoclonal Antibody (clone 12CA5)

Jean-Marc Egly, et al. Antibodies (Basel). 2020 Nov 12;9(4):61. doi: 10.3390/antib9040061. PMID: 33198305; PMCID: PMC7665837
“The reproducibility crisis in biological research has highlighted the critical need for well-defined, standardized affinity reagents. Recombinant antibodies, which are generated from sequenced clones, offer a scalable and consistent alternative to traditional polyclonal antibodies. This review explores the advantages of adopting recombinant technology in neuroscience, ensuring that experimental results remain valid and comparable across different laboratories.”
(Tags: Recombinant Antibodies; Neuroscience Research)
Katsumasa Irie, et al. ACS Meas Sci Au. 2024 Jun 19;4(3):230-238. doi: 10.1021/acsmeasuresciau.4c00008. PMID: 38927891; PMCID: PMC11196025
“Achieving high-precision molecular counting in super-resolution microscopy requires efficient antibody dissociation to ensure target accessibility. By introducing chaotropic agents to the imaging buffer, the noncovalent interactions between the Hemagglutinin (HA) tag and its specific monoclonal antibody fragment can be precisely modulated. This method significantly improves the dynamic range and reliability of quantitative molecular census measurements in fixed-cell samples.”
(Tags: Super-resolution Imaging; HA-Tag)
Takushi Miyoshi, et al. Methods Mol Biol. 2024;2722:155-167. doi: 10.1007/978-1-0716-3477-6_11. PMID: 38349454; PMCID: PMC10859171
“Traditional immunolabeling strategies often utilize multiple antibodies, which can increase the probe size and compromise spatial resolution in super-resolution microscopy. Single-antibody labeling approaches minimize these artifacts, allowing for more faithful structural reconstructions of cellular components. This protocol details the optimization of labeling density and binding kinetics to maximize the performance of super-resolution techniques.”
(Tags: Immunolabeling; Super-resolution Microscopy)
M. C. H. M. van der Wal, et al. Elife. 2018 Sep 6;7:e36873. doi: 10.7554/eLife.36873. PMID: 30188307; PMCID: PMC6125123
“Understanding the organization and interactions of surface proteins is essential for elucidating cellular signaling mechanisms. The two-hybrid antibody micropattern assay serves as a robust platform for detecting protein-protein associations, such as those occurring between MHC class I heavy chains, directly on the cell membrane. Results from this study confirm that these specific 'in cis' interactions can be systematically mapped using this high-resolution methodology.”
(Tags: Micropattern Assay; Protein-Protein Interaction)
T. Miyoshi, et al. STAR Protoc. 2023 Sep 15;4(3):102488. doi: 10.1016/j.xpro.2023.102488. PMID: 37647183; PMCID: PMC10468357
“Multiplexed imaging techniques require probes with tunable binding kinetics, specifically fast-dissociating antibody fragments that allow for repeated labeling and washing cycles. This protocol provides a step-by-step guide for the engineering and validation of such probes, optimized for high-throughput super-resolution microscopy. These tools facilitate the simultaneous visualization of multiple targets within complex biological structures.”
(Tags: Multiplexed Imaging; Antibody Engineering)

Other Tag Antibodies:
DYKDDDDK-Tag Monoclonal Antibody (Clone L1.1)
Myc-Tag Monoclonal Antibody (Clone 9E10)
HA-Tag Monoclonal Antibody (Clone 12CA5)
V5-tag Monoclonal Antibody (Clone SV5-Pk1)
Strep-Tag Monoclonal Antibody (Clone C23.21)
His-Tag Monoclonal Antibody (Clone 3D5)
His-tag Monoclonal Antibody (Clone HIS.H8)
GFP-tag Monoclonal Antibody
RFP-tag Monoclonal Antibody
His-Tag Monoclonal Antibody (Clone 26D11)

Loading Control Antibodies:
GAPDH Monoclonal Antibody
beta-Actin Monoclonal Antibody
beta-Tubulin Monoclonal Antibody

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