My Cart [ 0 ]
Home > Molecular Biology > PCR > PCR Master Mixes > Cell and Tissue Direct PCR Kit

Cell and Tissue Direct PCR Kit

Cell and Tissue Genomic DNA Extraction Coupled with PCR Kits
Catalog No. Product Name Size List Price (US$) Quantity
MB103-11 Cell and Tissue Direct PCR Kit 100 reactions 109.00
Description

Cell and Tissue Direct PCR Kit = Rapid Cell and Tissue Genomic DNA Extraction Kit (Catalog No. MB103-10) + 2x PCR PreMix, with dye (Catalog No. MB067-EQ2-100). The kits and reagents are routinely used in mutation detection, SNP genotyping, and target detection in transgenic animals.

Rapid genomic DNA extraction:

Step 1:

A) Mouse tails: Place 2-3 mm mouse tail in a 0.5 ml microcentrifuge tube containing 100 ul solution A.
B) Cultured cells: Add 1x10^4 - 1x10^5 cell suspension in <10 ul to a 0.5 ml microcentrifuge tube containing 100 ul solution A or add 100 ul solution A to a cell pellet that contains 1x10^4 - 1x10^5 cells.

Step 2:

Place the tube in a thermocycler and heat at 95°C for 5 min. Cool the tube to room temperature. Add 10 ul solution B and vortex briefly. Extracted genomic DNA is now ready for use as PCR temperate or for longer term storage at -20°C.

Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.

Related Links

Super savings and terrific tips sent to your email inbox:

See our Privacy Policy