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Rapid PCR Master Mix

Catalog No. Product Name Size List Price (US$) Quantity
MB049-EQ2G-L 2x Rapid PCR Master Mix (Green Dye) 1000 x 20 ul reactions 110.00
MB049-EQ2G-XL 2x Rapid PCR Master Mix (Green Dye) 5000 x 20 ul reactions 400.00
Description

MB049-EQ2G: 2x Rapid PCR Master Mix, with green dye

The cheap 2x rapid PCR Master Mix with non-interfering green dye is ready-to-use 2x concentrated solution for genotyping and DNA subcloning. Except DNA template and primers, the 2x rapid PCR master mix contains all other components for PCR, including Taq DNA polymerase, extension promoter, dNTPs, MgCl2, reaction buffer, PCR stabilizer and enhancer at optimal concentrations for consistent and efficient PCR amplification. For a 20 ul PCR reaction, simply add 10 ul of 2x rapid PCR master mix to specific primers, DNA template, and water. No additional loading buffer is required for electrophoresis when the PCR reaction is completed. This product contains electrophoresis buffer and green dye. It can be directly electrophoresed after the reaction, which is convenient to use. The protective agent included allows the 2x rapid PCR Master Mix to maintain stable activity after repeated freezing and thawing.

The amplification speed of the 2x rapid PCR master mix is ~15 sec/kb, a standard for rapid PCR reaction. The speed can even reach 1 sec/kb if the amplification length is <1 kb. The 2x rapid PCR master mix has excellent amplification performance and high storage stability. It is suitable for PCR amplification within 5 kb if genomic DNAs are used as the template, and 10 kb if plasmid and λDNA are used as the template. There is an A at the 3’ end of the PCR product, which can be directly cloned into the T vector and is suitable for our one-step homologous recombination-based DNA subcloning kit.

Optimal template and primer concentrations, and PCR program parameters should be determined experimentally by the investigator.

Features of the 2x rapid PCR master mix for genotyping:
* The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors.
* The tracking dye and precipitant have been added into the PreMix so that the PCR product can be directly loaded for electrophoresis.
* Fast amplification speed: normally ~15 sec/kb; ~1 sec/kb if the amplification length is <1 kb.
* High stability: repeated freezing and thawing for 50 times without significant decrease in activity.
* Low cost: $440 for 50 ml 2x rapid PCR PreMix with dye. The cheapest 2x rapid PCR master mix in the market.

Applications of the 2x rapid PCR master mix:
* Routine PCR using genomic, viral and plasmid templates.
* RT-PCR.
* DNA cloning and subcloning.
* Primer extension.
* DNA and colony screening.
* Genotyping.
* DNA mutagenesis.
* Generating hybridization probes for Southern or Northern hybridization.

The Taq DNA polymerase (catalog No. MB042-EUT, $1250 for 50000 units or 10 ml) contained in the 2x rapid PCR master mix with green dye is available alone.

Storage/Stability: Stable for 1 year at -20°C and 1 week at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.

PCR enzymes:
* Taq DNA polymerase.
* High yield Taq DNA polymerase.
* Bst DNA polymerase large fragment.
* Hot-start high-fidelity DNA polymerase.
* Hot start Taq DNA polymerase with chemical or antibody inhibitor, used for SYBR green or Taqman probe-based real time PCR (qPCR or quantitative PCR).

2x PCR master mixes:
* 2x PCR preMix with dye (green, red, blue).
* 2x rapid PCR master mix with a green dye.
* 2x long PCR master mix with a blue dye.
* 2x high yield PCR master mix with a blue dye.
* 2x hot-start high-fidelity PCR master mix with a blue dye.
* Blood direct PCR kit
* Cell and tissue direct PCR kit
* RT supermix for qPCR.
* RT-PCR Kit.
* Dye-based one-step qRT-PCR kits.
* Probe-based one-step qRT-PCR kits.
* Probe-based multiplex one-step qRT-PCR kits.
* SYBR Green qPCR master mixes.
* Probe qPCR master mixes.

PCR components and related products:
* dNTP mix, 10 mM each and dNTP mix, 25 mM each.
* Proteinase K powder and Proteinase K solution.
* Reverse transcriptases and First strand cDNA synthesis kits.
* Murine RNase inhibitor.
* Uracil DNA glycosylases.
* PCR enhancer.

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