MB112: Proteinase K Solution, PCR Grade

Proteinase K is commonly used for protein digestion, DNA extraction, RNA purification, direct PCR, and genotyping. The recombinant proteinase K enzyme is a mutant to the native protease, which gains higher specific activity and yield as well as wider pH and temperature range . The large scale recombinant enzyme production has advantage in lot-to-lot consistency, superior purity and cost-efficiency. DNA-free nature of recombinant Proteinase K made it well-suited in isolating PCR and RT-PCR templates.
High purity: Each lot of the recombinant proteinase K solution (molecular biology grade, PCR grade, NGS grade, chip grade, and RNA grade) was tested to ensure the absence of DNases and RNases, and free of DNA.
The cheapest proteinase K solution in the market.
Specific activity:  ≥ 780 units/ml.

MB112: Proteinase K Solution, PCR Grade

Proteinase K is commonly used for protein digestion, DNA extraction, RNA purification, direct PCR, and genotyping. The recombinant proteinase K enzyme is a mutant to the native protease, which gains higher specific activity and yield as well as wider pH and temperature range . The large scale recombinant enzyme production has advantage in lot-to-lot consistency, superior purity and cost-efficiency. DNA-free nature of recombinant Proteinase K made it well-suited in isolating PCR and RT-PCR templates.
High purity: Each lot of the recombinant proteinase K solution (molecular biology grade, PCR grade, NGS grade, chip grade, and RNA grade) was tested to ensure the absence of DNases and RNases, and free of DNA.
The cheapest proteinase K solution in the market.
Specific activity:  ≥ 780 units/ml.

MB049-EQ2G: 2x Rapid PCR Master Mix, with green dye

* The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors.
* The tracking dye and precipitant have been added into the PreMix so that the PCR product can be directly loaded for electrophoresis.
* Fast amplification speed: normally ~15 sec/kb; ~1 sec/kb if the amplification length is <1 kb.
* High stability: repeated freezing and thawing for 50 times without significant decrease in activity.
* Low cost: $400 for 50 ml 2x rapid PCR PreMix with dye. The cheapest PCR master mix for genotyping in the market.

- Reliable results. Through development of genome-wide qPCR primers, we have successfully tested over 30,000 genes in qPCR assays. Relative and absolute analysis of gene expression by qPCR is available for all known genes in any organism.
- Save money. No need to buy real-time PCR instruments or reagents. Our reverse transcription and qPCR reagents are used to save clients' cost.

MB036-5G: 5G Reverse Transcriptase

* Extensive template compatibility: compatible with various templates such as animals, plants, and viruses.
* Super impurity tolerance: tolerant to common impurities (ethanol, isopropanol, water balance phenol, guanidine isothiocyanate, humic acid).
* Higher reverse transcription efficiency than 4G reverse transcriptase.

MB036-4G: 4G Reverse Transcriptase

* Extensive template compatibility: compatible with various templates such as animals, plants, and viruses.
* Super impurity resistance: resistant to common impurities (ethanol, isopropanol, water balance phenol, guanidine isothiocyanate, humic acid).
* Long-fragment cDNA amplification (as long as 20 kb).

MB036-MM: M-MLV(H-) Reverse Transcriptasee

* Point mutations eliminate RNase H activity to obtain long cDNA products ≤5 kb.
* Stable and reliable reverse transcription performance for RNA templates above 100 ng.
* For 5’-RACE reaction and cDNA library construction.

MB079: Recombinant Murine RNase Inhibitor (RI)

* High purity;
* Neither DNases nor RNases present;
* Compatible with RT-PCR and RT-qPCR.

MB127-5G: 5G RT Supermix for qPCR with gDNA Elimination Function

* The 5G RT supermix for qPCR with gDNA elimination function is suitable for two-step RT-qPCR detection, and compatible with dye-based and probe-based qPCR, enabling high-performance gene expression analysis.
* Simple and fast operation: one-step genome clearance and reverse transcription.
* Higher reverse transcription efficiency and higher cDNA yields from all regions of RNA transcripts: based on the more efficient 5G Reverse Transcriptase.
* Flexible choice of primers: Different types of reverse transcription primers can be used flexibly for different experimental designs.
* Excellent cDNA stability: complete deactivation of thermal DNase and long-term storage of cDNA.
 

MB127-4G: 4G RT Supermix for qPCR with gDNA Elimination Function

* The 4G RT supermix for qPCR with gDNA elimination function is suitable for two-step RT-qPCR detection, and compatible with dye-based and probe-based qPCR, enabling high-performance gene expression analysis.
* Based on the highly efficient 4G Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA.
* Quick and complete removal of genome contamination by the 4 × gDNA Removing Buffer: to ensure more reliable follow-up results, and simplify the design of qPCR primers, without the need to design primers across introns.
* Different primers are selected for different downstream applications.

MB038-4G: 4G One-step RT-PCR Kit

MB038-4Gb: 4G One-step RT-PCR Kit with blue dye

The kit is provided in a convenient Master Mix format. The 2 x One-step Mix contains optimized buffer system, dNTP and loading dye; One-step Enzyme Mix contains optimized ratio of 4G Reverse Transcriptase, Murine RNase inhibitor, and hot start Taq DNA polymerase.

* High efficiency. Integrating the superior performance of 4G Reverse Transcriptase and hot-start Taq DNA Polymerase, with an optimized buffer system, the detection sensitivity of the 4G One Step RT-PCR Kit can reach 1 pg total RNA and can be amplified Fragments up to 10 kb.
* High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
* Reproducible results.
* Reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which improves detection throughput and reduces the risk of contamination.

MB0110-PC: Spin Column for DNA/RNA Tiniprep

* No buffer retention, no dead volume.
* High recovery rate, High quality, fast and easy with 2 minute procedure.
* Small elution volume (down to 5 ul).
* Small loading and elution amount (down to 10 ng).
* For DNA/RNA nano-prep (10 ng to 20 ug) directly from single E.coli colony or other small samples.
* Broad range recovery of DNA and RNA from short oligo/micro RNA of 17 bp to 140 kb DNA.
* Compatible with most manufacturers’ RNA or DNA kit reagents.

MB092-PC: Spin Column for Gel Extraction

* Spin columns are compatible to reagents in gel extraction kits from most of other manufacturers, such as Qiagen and Sigma. The yield is similar.
* Competitive and attractive price: $85 for 200 universal spin columns.
* Syd Labs provides the recipe of reagents for gel extraction if you buy spin columns from us. Very simple to make those reagents. The shipping fee is much lower if you buy spin columns only rather than the whole DNA purification kits with heavy and bulky reagents.