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    37 products and services containing "taq"
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  • MB041-EN-1: dNTP Mix, 10 mM each

    Chemically synthesized and >99% purity confirmed by HPLC;
    Free of human and E. coli DNA;
    Free of DNases and RNases;
    Free from PCR, qPCR, and cDNA synthesis inhibitors;
    Stable for 2 years at –20°C.

  • MB066-EN-1: dNTP mix, 25 mM each

    MB066-EN-2: dNTP set, 100 mM each

    Chemically synthesized and >99% purity confirmed by HPLC;
    Free of human and E. coli DNA;
    Free of DNases and RNases;
    Free from PCR, qPCR, and cDNA synthesis inhibitors;
    Stable for 2 years at –20°C.
     

  • MB119-B: 2x hot-start high-fidelity PCR Master Mix, with blue dye
    * Simple operation: The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors. The tracking dye and precipitant have been added into the master mix so that the PCR product can be directly loaded for electrophoresis.
    * Ultra-high fidelity: The fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. For every 300,000 bases amplified, there are fewer than 5 mismatches.
    * Fast amplification: normally 4-5 sec/kb; ~0.5 sec/kb if the amplification length is <1 kb; 4-150 times that of conventional PCR master mix.
    * Long fragment amplification: up to 40 kb from simple templates such as λDNA and plasmids, up to 20 kb from complex templates such as genomic DNA, and up to 10 kb from cDNA templates.
    * Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples.
    * Features of the hot-start high fidelity DNA polymerase (catalog No. MB118) contained in the 2x hot-start high-fidelity PCR master mix.

  • MB121: Bst 2G DNA Polymerase, Large Fragment

    * Rapid amplification of diverse templates.
    * Higher amplification yield.
    * Increased tolerance of inhibitors including salts.
    * Improved thermostability of the enzyme.
    * The cheapest Bst 2.0 DNA Polymerase, Large Fragment in the market.
     

    MB122: Bst DNA Polymerase, Large Fragment

    * Warm-start reaction system: The reaction process is completed by simply mixing all components at 60 - 65 ℃ for 30 - 60 minutes.
    * Multiple options of reading results: The amplification products can be checked with gel electrophoresis, colorimetric methods, and fluorescence intensity methods.
    * Potent amplification ability: The upgraded polymerase features strong 5´→3´ DNA polymerase activity and strong strand displacement activity.
    * Perfect for assembling RT-LAMP kits: Compatible with various reverse transcriptases from Syd Labs and other commercial suppliers.

  • MB125: Universal DNA Assembly Cloning Kit

    * Simple and convenient: mix one or multiple linearized inserts and the vector in one tube, and incubate them for 5 to 15 minutes at 50°C. One tube, one step.
    * Fast and efficient with high positive rate and low background.
    * No restriction enzymatic site(s) is needed.
    * Multiple linearized inserts: 1 to 5.
    * Insert size: 50 bp to 10 kb.

     

    MB126: Simple DNA Assembly Cloning Kit

    * Simple and convenient: mix one linearized insert and the vector, and incubate them for 5 minutes at 50°C.
    * Fast and efficient with >95% positive rate.
    * No restriction enzymatic site(s) is needed.
    * Multiple linearized inserts: 1.
    * Insert size: 50 bp to 10 kb.

     

  • BP000768-ENZ-281: Recombinant Thermostable dUTPase Pyrococcus Fruriosus

    Source: E.coli-derived.
    Activity: 103 u/ug.
    Purity > 97%, by RP-HPLC and SDS-PAGE.

  • MB128: Dye-Based One-Step qRT-PCR Kit

    * Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination.
    * High detection sensitivity of one step qRT-PCR SYBR green kit: 1 pg total RNA.
    * More stable amplification curve with low concentration template.
    * Reduced formation of primer dimers and improved specificity of qPCR products.
    * Convenient, easy, and quick operation.

  • MB037-5G: 5G First Strand cDNA Synthesis Kit with gDNA Elimination Function

    * Higher reverse transcription efficiency and higher cDNA yields from all regions of RNA transcripts: based on the more efficient 5G Reverse Transcriptase.
    * Flexible choice of primers: Different types of reverse transcription primers can be used flexibly for different experimental designs.
    * Quick and complete removal of genome contamination by the 5× gDNA Removing Buffer: to ensure more reliable follow-up results, and simplify the design of qPCR primers, without the need to design primers across introns.

     

    MB037-4G: 4G First Strand cDNA Synthesis Kit with gDNA Elimination Function

    * Based on the highly efficient 4G Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA.
    * A wide range of template starting amount: from 1 pg - 5 μg total RNA.
    * Long fragment amplification: up to 15 kb or more.
    * Anchored Oligo (dT)23 VN primer is designed for binding site anchoring with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis.
    * Different primers are selected for different downstream applications. The synthesized one-strand cDNA is widely used in molecular cloning, hybridization, PCR amplification and qPCR reaction, and so on.

  • MB040-HY2: 2x HY PCR Master Mix, with blue dye

    * The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors.
    * The high yield PCR Master Mix has increased amplification robustness, fidelity, yield, and fragment length, as well as the ability to handle difficult or “dirty” templates.
    * The tracking dye and precipitant have been added into the PCR PreMix so that the PCR product can be directly loaded for electrophoresis.

    Features of the blend of Taq enzyme and a proofreading enzyme contained in the high yield PCR master mix:
    * Robust processivity. Up to 10 kb human genomic and 15 kb lamda DNA fragments have been tested for amplification.
    * High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
    * Reproducible results. No visible activity change after storage of the Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
    * Proofreading with 5′ exonuclease activity.

  • MB016-HYT: High Yield Taq DNA Polymerase

    * Robust processivity. Up to 10 kb human genomic and 15 kb lamda DNA fragments have been tested for amplification.
    * High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
    * Reproducible results. No visible activity change after storage of the high yield Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
    * Proofreading with 5′ exonuclease activity.

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