MB119-B: 2x hot-start high-fidelity PCR Master Mix, with blue dye
* Simple operation: The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors. The tracking dye and precipitant have been added into the master mix so that the PCR product can be directly loaded for electrophoresis.
* Ultra-high fidelity: The fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. For every 300,000 bases amplified, there are fewer than 5 mismatches.
* Fast amplification: normally 4-5 sec/kb; ~0.5 sec/kb if the amplification length is <1 kb; 4-150 times that of conventional PCR master mix.
* Long fragment amplification: up to 40 kb from simple templates such as λDNA and plasmids, up to 20 kb from complex templates such as genomic DNA, and up to 10 kb from cDNA templates.
* Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples.
* Features of the hot-start high fidelity DNA polymerase (catalog No. MB118) contained in the 2x hot-start high-fidelity PCR master mix.

EK0403: AAV2 Titration ELISA Kit

Application: for the quantitative determination of full and empty AAV2 capsids, including intact AAV2 Wild Type virions, AAV2 recombinant virions, assembled AAV2 virions, intact empty AAV2 capsids.
This assay is specific for AAV2 capsids.

Detection range: 1.0^7 capsids/mL - 6.5^8 capsids/mL
Sensitivity: 1.0^7 capsids/mL
Precision: CV < 10%

EK0401: AAV5 Titration ELISA Kit

Application: for the quantitative determination of full and empty AAV5 capsids, including intact AAV5 Wild Type virions, AAV5 recombinant virions, assembled AAV5 virions, intact empty AAV5 capsids.
This assay is specific for AAV5 capsids.

Detection range: 3.1^7 capsids/mL - 2.0^9 capsids/mL
Sensitivity: 1.5^7 capsids/mL
Precision: CV < 10%

EK0402: AAV8 Titration ELISA Kit

Application: for the quantitative determination of full and empty AAV8 capsids, including intact AAV8 Wild Type virions, AAV8 recombinant virions, assembled AAV8 virions, intact empty AAV8 capsids.
This assay is specific for AAV8 capsids.

Detection range: 1.0^7 capsids/mL - 6.5^8 capsids/mL
Sensitivity: 1.0^7 capsids/mL
Precision: CV < 10%

EK0400: AAV9 Titration ELISA Kit

Application: for the quantitative determination of full and empty AAV9 capsids, including intact AAV9 Wild Type virions, AAV9 recombinant virions, assembled AAV9 virions, intact empty AAV9 capsids.
This assay is specific for AAV9 capsids.

Detection range: 1.0^7 capsids/mL - 6.5^8 capsids/mL
Sensitivity: 1.0^7 capsids/mL
Precision: CV < 10%

EK000081-EK0557: Human ACE ELISA Kit

Range: 156pg/ml-10,000pg/ml
Sensitivity < 5 pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitive detection of human ACE in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: Four months at 4°C and eight months at -20°C.

EK000116-EK0305: Human ANG ELISA Kit

Range 78pg/ml-5000pg/ml
Sensitivity < 12 pg/ml
Specificity: No detectable cross-reactivity with any other cytokine.
Application: For quantitative detection of human ANG in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: Four months at 4°C and eight months at -20°C.

PA007158.m1: In Vivo Grade Recombinant Anti-DNP Mouse IgG1 Kappa Isotype Control Antibody (mIgG1 Isotype Control)

The in vivo grade recombinant anti-DNP mouse IgG1 isotype control antibody (clone of DNP.m1) was produced in mammalian cells.
Applications: an isotype-matched negative control for anti-DNP mouse IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Please contact us to ask for a quote for the engineered DNP isotype control mutants, isotype control and mutants with Avi-, His-, and Flag-tags, and biotinylated isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the anti-DNP isotype control and mutants are available.

100000 cells per hybridoma clone are sufficient for cloning and sequencing the monoclonal antibody variable regions (both VH and VL) and full length. Welcome to challenge us with smaller number of clonal B cells or non-viable cells for hybridoma rescue.

- Reliable results. With optimized workflow and PCR primers, we have successfully cloned and sequenced >3000 antibodies from various species in the past 10 years. Syd Labs has become a reliable CRO supplier of antibody sequencing to biotech and biopharma companies, government agencies including FDA and NIH, and universities. Clients' samples of hybridoma cell lines or clonal B cells just need to meet 2 requirements: functional antibodies are secreted by the cells; enough RNA remains.
- Save time. One may learn how to clone and sequence antibody genes by studying hybridoma antibody cloning and antibody sequencing protocols that we collected from public literature. Generally speaking, it is more difficult to clone antibody genes from rodents than other species; among isotypes, it is the easiest to clone IgG genes, especially IgG1. A couple of companies sell such kits and PCR primers. However, it takes time to become a highly efficient and experienced professional for so many lab skills and bioinformatics analysis involved in such projects. In addition, there are some samples invaluable but difficult to work. Some service suppliers spend months but could not solve the problem, just wasting clients' samples and time.
- Save money. Since we process samples from multiple clients in the same time, our cost is lower than clients'. Our low cost molecular biology reagents further save clients' cost.

Kinetic analysis is used to determine the affinity of an antibody to its soluble antigen using surface plasmon resonance (SPR) or biolayer interferometry (BLI), which can measure kon, koff, and kD. Association and dissociation are measured on the Biacore T100 or the ForteBio Octet QKe using various concentrations of the antigen and the kD is calculated using an appropriate curve-fitting model.

Fast turnaround time: 2-3 days per antibody-antigen pair, but may require more time depending on the complexity of analysis.