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EK000116-EK0305: Human ANG ELISA Kit
Range 78pg/ml-5000pg/ml
Sensitivity < 12 pg/ml
Specificity: No detectable cross-reactivity with any other cytokine.
Application: For quantitative detection of human ANG in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: Four months at 4°C and eight months at -20°C.
Background
Angiogenin (Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene.[1] Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease.[2] Hooper et al. (2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al. (1989, 1990) assigned the human angiogenin gene to chromosome 14q11.
Principle
Human ANG ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human ANG specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ANG amount of sample captured in plate.
Reference
1. ^ Weremowicz S, Fox EA, Morton CC, Vallee BL (1990). "Localization of the human angiogenin gene to chromosome band 14q11, proximal to the T cell receptor alpha/delta locus". Am J Hum Genet 47 (6): 973?1.
2. "Entrez Gene: ANG angiogenin, ribonuclease, RNase A family, 5". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=283.
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