Anti-DNP Isotype Control Antibodies

Background

The anti-DNP antibodies specifically bind to DNP (2,4,6-trinitrophenyl) and derivatives. The DNP antibodies have been used in various applications, such as ELISA, Immunohistochemistry, Immunocytochemistry, and Western Blot. Because Dinitrophenyl (DNP) is a hapten and not found endogenously in animal and plant tissues, the anti-DNP monoclonal antibody in nature is suitable for use as an isotype-matched control antibody to estimate the degree of non-specific binding by an antigen-specific antibody. The recombinant mouse IgG1 kappa isotype control (clone number: DNP.m1) and mutants (natural and engineered mIgG1 isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. The isotypes of mouse IgG antibody reagents used in scientific research and clinical diagnostics are mainly mouse IgG1 kappa. Mouse IgG1 antibodies and engineered mutants are also used in therapeutic drug discovery. Mouse IgG1 antibodies are more like human IgG4 antibodies than human IgG1 antibodies, which behave more like mouse IgG2a or IgG2c antibodies. Many antibody drugs especially those targetting immune checkpoint proteins use the human IgG4 constant regions.

As an ideal recombinant mouse IgG1 isotype control,  the recombinant anti-DNP mIgG1 kappa isotype control has low or no specific binding to any mouse sample, and has completely mouse variable regions and the mouse IgG1 kappa constant regions. The anti-DNP mouse IgG1 kappa isotype control and mutants can be used in ELISA, WB, IHC, the cell-based assay or animal model as the antibody negative control.

Please contact us to ask for a quote for the engineered anti-DNP isotype control mutants, isotype control and mutants with Avi-, His-, and DYKDDDDK-tags, and biotinylated isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the anti-DNP isotype control and mutants are available.

PA007158.m1: In Vivo Grade Recombinant Anti-DNP Mouse IgG1 Kappa Isotype Control Antibody (mIgG1 Isotype Control)

The in vivo grade recombinant anti-DNP mouse IgG1 isotype control antibody (mIgG1 isotype control) was produced in mammalian cells.
Specificity: Dinitrophenyl (DNP).
Clone: DNP.m1.
Isotype: mouse IgG1, kappa.
Applications: an isotype-matched negative control for anti-DNP mouse IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant anti-DNP mouse IgG1 kappa isotype control antibody (clone of DNP.m1) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

References of Anti-DNP Isotype Control Antibodies:

Antibody Isotype Switching as a Mechanism to Counter HIV Neutralization Escape.
Scheepers, C., et al. Cell Rep. 2020 Nov 24;33(9):108448. doi: 10.1016/j.celrep.2020.108448. PMID: 33238132
This study demonstrates that IgG3 and IgA1 isotypes exhibit superior neutralization of longitudinal autologous HIV viruses and epitope mutants compared to IgG1. Additionally, it highlights that detrimental class-switch recombination (CSR) events leading to reduced neutralization can be reversed through further CSR, termed "switch redemption." CSR thus serves as an additional immunological mechanism to counter viral escape from HIV-specific antibody responses. The study also notes that somatic hypermutation (SHM) in immunoglobulin variable regions enables maturing antibodies to neutralize emerging viral escape variants, while the Ig-constant region, determining isotype, influences epitope recognition.
Tags: activity of anti-DNP isotype control; activity of anti-DNP isotype control mAb

A universal dual mechanism immunotherapy for the treatment of influenza virus infections.
Liu, X., et al. Nat Commun. 2020 Nov 4;11(1):5573. doi: 10.1038/s41467-020-19386-5. PMID: 33149136
This study evaluates the ability of zan-DNP to recruit anti-DNP antibodies to influenza A(H1N1)-infected cells, using biotinylated anti-DNP antibodies for visualization on MDCK cells. Confocal micrographs confirmed that anti-DNP antibodies bind only to infected cells treated with zan-DNP. In vivo, mice challenged with 100x MLD50 of A/California/07/2009 (H1N1)pdm09 virus and treated with 0.5 ?mol/kg zan-DNP or zanamivir plus anti-DNP antibodies (5 mg/kg, i.v.) showed that 24.4% of infected lung cells were opsonized with antibody in zan-DNP-treated mice. The study also tested co-injection of exogenous anti-DNP antibodies to compensate for low endogenous anti-DNP antibody titers, demonstrating an effective anti-influenza immune response.
Tags: anti-DNP isotype control antibody; anti-DNP isotype control antibody for animal model

Hapten-mediated recruitment of polyclonal antibodies to tumors engenders antitumor immunity.
Schrand, B., et al. Nat Commun. 2018 Sep 21;9(1):3886. doi: 10.1038/s41467-018-05566-x. PMID: 30242248
This study investigates the use of VEGF-DNP hapten conjugates to recruit polyclonal antibodies to tumors, demonstrating that polyclonal antibodies are more effective than monoclonal anti-DNP antibodies in eliciting tumor immunity. Experiments showed that VEGF-DNP, but not control aptamer-DNP conjugates, inhibited tumor growth in DNP-immune (DNP-KLH) mice but not in mock (KLH)-vaccinated mice. The VEGF-DNP conjugate incubated with monoclonal anti-DNP or isotype control antibody (D8406, SIGMA-Aldrich) at a 2:1 ratio was largely ineffective. Statistical analysis showed significant tumor inhibition with VEGF-DNP in DNP-KLH mice (p = 0.0066, Student’s t-test, n = 1).
Tags: anti-DNP isotype control antibody for flow cytometry; anti-DNP isotype control antibody in vivo

Antibodies with idiotypic and anti?idiotypic reactivity (epibodies) in conventional immune responses to dinitrophenylated carriers.
Muhumuza, L., et al. Immunology. 1998 Jul;94(3):391-400. doi: 10.1046/j.1365-2567.1998.00466.x. PMID: 9687174
This study found that only 7% of hybrid supernatants from spleens undergoing a primary response to DNP-Ba exhibited both Ab1 and Ab2 reactivity. After cloning, monoclonal antibodies (mAbs) were characterized for isotype and reactivity to DNP-BSA, rabbit anti-DNP antibodies, and unrelated antigens. Approximately half of the mAbs from the primary response to DNP-Ficoll were IgM and polyreactive. All mAbs with dual reactivity from DNP-Ba responses were also polyreactive and belonged to the IgM class, fitting the definition of epibodies with specificity for both the hapten and anti-hapten antibody.
Tags: anti-DNP isotype control for cancer research; anti-DNP isotype control for mouse tumor model

125 I-labeled anti-2, 4-dinitrophenyl (DNP)-antibodies: A universal reagent for solid phase radioimmunoassays.
Givol, D., et al. Mol Immunol. 1979 Oct;16(10):853-7. doi: 10.1016/0161-5890(79)90126-3. PMID: 92597
This study describes the development of solid phase radioimmunoassays (RIA) using 125I-labeled anti-DNP antibodies to quantify antigen-bound antibodies. The method employs dinitrophenylated antibodies, which are detected by the attachment of 125I-labeled anti-DNP, offering advantages such as using a single radiolabeled antibody for all tests, amplified tracer binding, and eliminating the need for immunochemical purification of each antibody. This approach allows haptenated immunoglobulins to serve as a general tracer in solid-phase RIA.
Tags: function of anti-DNP isotype control antibody; anti-DNP isotype control antibody mouse dose range

For more references about anti-DNP isotype control antibodies please contact our scientific support team with message@sydlabs.com.

In Vivo Grade Recombinant anti-DNP Isotype Control Antibodies (clone DNP.m1):
In vivo Grade Recombinant Anti-DNP Mouse IgG1 Isotype Control Antibody Mutants
In vivo Grade Recombinant Anti-DNP Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Anti-DNP Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Anti-DNP Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Anti-DNP Mouse IgG3 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Anti-DNP Mouse Fab Fragment
In vivo Grade Recombinant Anti-DNP Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Anti-DNP Human IgG2 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Anti-DNP Human IgG3 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Anti-DNP Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Anti-DNP Human Fab Fragment
In vivo Grade Recombinant Anti-DNP Human IgE Isotype Control Antibody
In vivo Grade Recombinant Anti-DNP Human IgM Isotype Control Antibody
In vivo Grade Recombinant Anti-DNP Human IgA1 Isotype Control Antibody
In vivo Grade Recombinant Anti-DNP Rat IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Anti-DNP Rat IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Anti-DNP Rat IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Anti-DNP Rat IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Anti-DNP Rat Fab Fragment
In vivo Grade Recombinant Anti-DNP Hamster IgG1 Isotype Control Antibody
In vivo Grade Recombinant Anti-DNP Hamster IgG2 Isotype Control Antibody
In vivo Grade Recombinant Anti-DNP Rabbit IgG Isotype Control Antibody
In vivo Grade Recombinant Anti-DNP Ferret IgG1 Isotype Control Antibody
In vivo Grade Recombinant Anti-DNP Ferret IgM Isotype Control Antibody
In vivo Grade Recombinant Anti-DNP Ferret IgA Isotype Control Antibody
In vivo Grade Recombinant Anti-DNP Canine IgG1 Isotype Control Antibody
In vivo Grade Recombinant Anti-DNP Canine IgG2 Isotype Control Antibody
In vivo Grade Recombinant Anti-DNP Canine IgG3 Isotype Control Antibody
In vivo Grade Recombinant Anti-DNP Canine IgG4 Isotype Control Antibody
In vivo Grade Recombinant Anti-DNP Feline IgG1 Isotype Control Antibody

Recombinant anti-DNP Monoclonal Antibodies:
Recombinant Anti-DNP Monoclonal Antibodies (Clone DNP.m2)
In vivo Grade Recombinant Anti-DNP Monoclonal Antibodies (Clone MOPC 315)
In vivo Grade Recombinant Anti-DNP Monoclonal Antibodies (Clone SPE7)

Other Recombinant IgG Reference Antibodies:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG3 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody