PA007459: In vivo Grade Recombinant Anti-Human CD47 Monoclonal Antibody, Mouse IgG1 Kappa (Clone: B6H12)

The in vivo grade recombinant anti-human CD47 Mouse IgG1 Kappa monoclonal antibody was produced in mammalian cells. 
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: B6H12) specifically binds to human CD47.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD47 protein.

BP005040: Mouse CD47 Protein with the C-His Tag

Source: The recombinant Mouse CD47 Protein, (amino acids Gln19-Lys140) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: The affinity constant of 1.33 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004182: Mouse CD47 Protein with the C-hFc Tag

Source: The recombinant Mouse CD47 Protein, (amino acids Gln19-Lys140) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Mouse SIRP alpha protein with the C-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Mouse CD47 protein with the C-hFc tag with the EC50 of 0.26 ug/ml determined by ELISA (QC Test). The affinity constant of 28.33 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP003206: Human CD47 Protein with No Tag

Source: The recombinant Human CD47 Protein, (amino acids Gln19-Pro139) , was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD47 protein with No tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human SIRP alpha protein with the C-hFc tag with the EC50 of 0.24 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005366: Human CD47 Protein with the C-His Tag

Source: The recombinant Human CD47 Protein, (amino acids Gln19-Pro139) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD47 protein with the C-His tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human SIRP alpha protein with the C-hFc tag with the EC50 of 82.1 ng/ml determined by ELISA (QC Test). The affinity constant of 18.9 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005367: Human CD47 Protein with the C-hFc Tag

Source: The recombinant Human CD47 Protein, (amino acids Gln19-Pro139) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human SIRP alpha protein with the C-hFc tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human CD47 protein with the C-hFc tag with the EC50 of 5.0 ng/ml determined by ELISA (QC Test). The affinity constant of 13.8 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP004107: Cynomolgus/Rhesus macaque CD47 Protein with the C-His Tag

Source: The recombinant Cynomolgus/Rhesus macaque CD47 Protein, (amino acids Gln19-Glu141) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Cynomolgus/Rhesus macaque CD47 protein with the C-His tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human SIRP alpha protein with the C-hFc tag with the EC50 of 0.52 ug/ml determined by ELISA (QC Test). The affinity constant of 63.27 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005428: Biotinylated Human CD47 Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human CD47 Protein, (amino acids GIn19-Pro139) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human SIRP alpha protein with the C-His-Avi tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Human CD47 protein with the C-His tag with the EC50 of 0.48 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP003102: Biotinylated Human CD47 Protein with the C-hFc-Avi Tag

Source: The recombinant Biotinylated Human CD47 Protein, (amino acids Gln19-Pro139) fused with a hFc-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity:
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP003213: Rat CD47 Protein with the C-hFc Tag

Source: The recombinant Rat CD47 Protein, (amino acids Gln19-Lys140) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Mouse SIRP alpha protein with the C-hFc tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Rat CD47 protein with the C-hFc tag with the EC50 of 29.8 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005321: Human SIRP alpha/CD172a Protein with the C-His Tag

Source: The recombinant Human SIRP alpha/CD172a Protein, (amino acids Glu31-Arg370) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human SIRP alpha protein with the C-His tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human CD47 protein with the C-hFc tag with the EC50 of 99.1 ng/ml determined by ELISA (QC Test). The affinity constant of 20.6 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005320: Human SIRP alpha/CD172a Protein with the C-hFc Tag

Source: The recombinant Human SIRP alpha/CD172a Protein, (amino acids Glu31-Arg370) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD47 protein with the C-hFc tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human SIRP alpha protein with the C-hFc tag with the EC50 of 82.1 ng/ml determined by ELISA (QC Test). The affinity constant of 18.9 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP001107-C385: Recombinant Human Signal-Regulatory Protein α?-1 (SIRPA) Protein

Source: HEK 293-derived.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug).

BP005782: Human SIRP alpha V4 Protein with the C-His-Avi Tag

Source: The recombinant Human SIRP alpha V4 Protein, (amino acids Glu31-Arg370(E33G, L44S, T50S, T52I, R54H)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human SIRP alpha V4 protein with the C-His-Avi tag at 1 ug/ml (100 ul/Well). Dose response curve for the recombinant Human CD47 protein with the C-hFc tag with the EC50 of 44.3 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005744: Human SIRP alpha V2/CD172a Protein with the C-His Tag

Source: The recombinant Human SIRP alpha V2/CD172a Protein, (amino acids Glu31-Arg369) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human SIRP alpha V2 protein with the C-His tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human CD47 protein with the C-hFc tag with the EC50 of 6.7 ng/ml determined by ELISA (QC Test). The affinity constant of 9.11 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.

BP005755: Human SIRP alpha V2/CD172a Protein with the C-hFc Tag

Source: The recombinant Human SIRP alpha V2/CD172a Protein, (amino acids Glu31-Arg369) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Activity: Immobilized recombinant Human CD47 protein with the C-hFc tag at 2 ug/ml (100 ul/Well). Dose response curve for the recombinant Human SIRP alpha V2 protein with the C-hFc tag with the EC50 of 41.9 ng/ml determined by ELISA (QC Test).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.