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Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal recombinant Human CD47 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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BP003206: Human CD47 Protein with No Tag
Source: The recombinant Human CD47 Protein, (amino acids Gln19-Pro139) , was produced in HEK 293 cells.
Molecular Weight: The recombinant Human CD47 Protein has a predicted MW of 13.72 kDa. Due to glycosylation, the protein migrates to 35-48 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human CD47 protein with No tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human SIRP alpha protein with the C-hFc tag with the EC50 of 0.24 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human CD47 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
BP005366: Human CD47 Protein with the C-His Tag
Source: The recombinant Human CD47 Protein, (amino acids Gln19-Pro139) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human CD47 protein with the His tag at the C-terminus has a predicted MW of 14.5 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human CD47 protein with the C-His tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human SIRP alpha protein with the C-hFc tag with the EC50 of 82.1 ng/ml determined by ELISA (QC Test). The affinity constant of 18.9 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human CD47 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
BP005367: Human CD47 Protein with the C-hFc Tag
Source: The recombinant Human CD47 Protein, (amino acids Gln19-Pro139) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human CD47 protein with the hFc tag at the C-terminus has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human SIRP alpha protein with the C-hFc tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human CD47 protein with the C-hFc tag with the EC50 of 5.0 ng/ml determined by ELISA (QC Test). The affinity constant of 13.8 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human CD47 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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