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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal SIRPA Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
Signal regulatory protein alpha (SIRPα, designated CD172a), also known as Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS-1), CD172 antigen-like family member A, is a monomeric ~90 kDa type I transmembrane glycoprotein. The 504 aa human SIRPA contains two Ig-like C1-type domains and one Ig-like V-type domain. SIRPα can express in various tissue, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPα acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRPα engagement generally produces a negative regulatory signal, it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation.
BP001107-C385: Recombinant Human Signal-Regulatory Protein α-1 (SIRPA) Protein
Source: HEK 293-derived.
Recombinant Human Signal regulatory protein alpha (SIRPA) Protein is expressed with sequence (Glu31 Arg370) of Human SIRPA fused with a polyhistidine tag at the C-terminus.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug).
Formulation: Lyophilized from a 0.2 um filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
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