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Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2a Monoclonal Antibody.
Clone: DATK32.
Isotype: Rat IgG2a kappa.
Source: The anti-mouse LPAM-1 (Intergrin α4β7) monoclonal antibody (clone: DATK32) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: DATK32) specifically binds to mouse LPAM-1 (Intergrin α4β7) .
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse LPAM-1 (Intergrin α4β7) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse LPAM-1 (Intergrin α4β7) monoclonal antibody of clone DATK32 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Limited clonal relatedness between gut IgA plasma cells and memory B cells after oral immunization.
Bemark, M., et al. Nat Commun. 2016 Sep 6;7:12698. doi: 10.1038/ncomms12698. PMID: 27596266
Expression of the gut homing ?4?7 receptor (LPAM integrin) in GFP+ memory B cells, as opposed to that found in GFP? B cells or non-B cells in the PP and Spl. ...Lymphocytes were isolated as described and stained with anti-mouse IgD PerCP-Cy5.5 (1:400, 405710), LPAM (integrin ?4?7) PE (1:100, 120605; (Biolegend, San Diego, CA), B220 V500 (1:400, 561227), CD19 APC-H7 (1:200, 560143), CD80 PE (1:500, 553769), CD273 APC (1:200, 560086), CD138 PE (1:200, 553714), IgM PE-Cy7 (1:200, 552867; BD Biosciences), CCR9 PE-Cy7 (1:100, 25-1991), CD73 PE-Cy7 (1:50, 25-0731), IgA PE (1:50, 12-4204), GL7 eFluor 450 (1:100, 48-5902), CD38 Alexa700 (1:800, 56-0381), CD21/35 Pacific Blue (1:800, 57-0212; eBiosciences) or CCR10 APC (1:100, FAB2815A; R and D systems. Minneapolis, MN) and were analysed using LSR II (BD Biosciences) or Navios (Beckman Coulter, Brea, CA) flow cytometers. ...For blocking gut homing, we used the antibody clone DATK32 specific for the integrin ?4?7 (Bio X Cell, West Lebanon, NH); 250??g of the antibody was injected i.p. 3 days before, on the day of and 3 days after booster immunization. ...Orally primed mice were treated with ?4?7-blocking DATK32 Mab before and during p.o. or i.p. challenge immunizations with NP-CT and the number of GFP+ plasma cells in SI LP sections were determined (explained in Supplementary Fig. 6a). ...To address whether GC formation was needed for mucosal memory B-cell responses, we boosted p.o. primed mice after 6 months in the presence or absence of a GC-disrupting treatment with an anti-CD40L Mab in vivo.
Tags: clone DATK32 of anti-mouse LPAM-1 (Intergrin α4β7) mAb; DATK32 anti-mouse LPAM-1 (Intergrin α4β7) antibody
For more references about Anti-mouse LPAM-1 (Intergrin alpha 4 beta 7) Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
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