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Mouse CD47 Protein

CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6

Catalog No. Product Name Size List Price (US$) Quantity
BP005040 Mouse CD47 Protein with the C-His Tag 100UG 270.00
BP005040 Mouse CD47 Protein with the C-His Tag 500UG 900.00
BP004182 Mouse CD47 Protein with the C-hFc Tag 100UG 270.00
BP004182 Mouse CD47 Protein with the C-hFc Tag 500UG 900.00
Description

BP005040: Mouse CD47 Protein with the C-His Tag

Source: The recombinant Mouse CD47 Protein, (amino acids Gln19-Lys140) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Mouse CD47 protein with the His tag at the C-terminus has a predicted MW of 14.9 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Tris-Bis PAGE result.
Activity: The affinity constant of 1.33 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Mouse CD47 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP004182: Mouse CD47 Protein with the C-hFc Tag

Source: The recombinant Mouse CD47 Protein, (amino acids Gln19-Lys140) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Mouse CD47 protein with the hFc tag at the C-terminus has a predicted MW of 40.60 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Mouse SIRP alpha protein with the C-hFc tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Mouse CD47 protein with the C-hFc tag with the EC50 of 0.26 ug/ml determined by ELISA (QC Test). The affinity constant of 28.33 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Mouse CD47 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Mouse CD47 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

Syd Labs provides the following CD47 Protein :
Biotinylated Human CD47 Protein
CD47 Protein
Cynomolgus/Rhesus macaque CD47 Protein
Human CD47 Protein
Mouse CD47 Protein

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