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Home > Antibodies > Anti-Mouse Antibodies for Flow Cytometry > Anti-mouse CD28 Monoclonal Antibodies for Flow Cytometry

Anti-mouse CD28 Monoclonal Antibodies for Flow Cytometry

T-cell-specific surface glycoprotein CD28; TP44

Catalog No. Product Name Size List Price (US$) Quantity
PA007491.m1 Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: 37.51), Mouse IgG1 Kappa 100 ug 50.00
PA007491.m1 Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: 37.51), Mouse IgG1 Kappa 500 ug 100.00
PA007491.h1Fs Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: 37.51), Human IgG1 Fc Silent Kappa 100 ug 50.00
PA007491.h1Fs Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: 37.51), Human IgG1 Fc Silent Kappa 500 ug 100.00
PA007719.h1Fs-b Biotinylated Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: 37.51), Human IgG1 Fc Silent Kappa 100 ug 75.00
PA007719.h1Fs-b Biotinylated Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: 37.51), Human IgG1 Fc Silent Kappa 500 ug 150.00
Description

PA007491.m1: Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: 37.51), Mouse IgG1 Kappa

Recombinant Mouse IgG1 Monoclonal Antibody. 
Clone: 37.51.
Isotype: Mouse IgG1 Kappa.
Source: The anti-mouse CD28 monoclonal antibody (clone: 37.51) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 37.51 antibody specifically binds to mouse CD28.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007491.h1Fs: Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: 37.51), Human IgG1 Fc Silent Kappa

Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: 37.51.
Isotype: Human IgG1 kappa.
Source: The anti-mouse CD28 monoclonal antibody (clone: 37.51) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 37.51 antibody specifically binds to mouse  CD28.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007719.h1Fs-b: Biotinylated Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: 37.51), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain. 
Clone: 37.51.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated anti-mouse CD28 monoclonal antibody (clone: 37.51) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant 37.51 antibody specifically binds to mouse CD28.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The 37.51 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of anti-mouse CD28 antibody (37.51):

1、Targeting abatacept-resistant T-helper-17 cells by aldehyde dehydrogenase inhibition
Tokifuji Y, et al. iScience. 2023 Dec 9;27(1):108646. doi: 10.1016/j.isci.2023.108646. eCollection 2024 Jan 19. PMID: 38226171
IL-17-producing helper T (Th17) cells are long-lived and serve as central effector cells in chronic autoimmune diseases. The underlying mechanisms of Th17 persistence remain unclear...
Tags: 37.51 antibody T cell activation; anti-mouse CD28 clone 37.51 stimulation

2、A non-catalytic role of IPMK is required for PLCγ1 activation in T cell receptor signaling by stabilizing the PLCγ1-Sam68 complex
Hong S, et al. Cell Commun Signal. 2024 Oct 30;22(1):526. doi: 10.1186/s12964-024-01907-0. PMID: 39478550
Phospholipase C gamma 1 (PLCγ1) is an important mediator of the T cell receptor (TCR) and growth factor signaling. PLCγ1 is activated by Src family kinases (SFKs) and produces inositol 1,4,5-triphosphate (InsP3 ) from phosphatidylinositol 4,5-bisphosphate (PIP 2)...
Tags: functional grade 37.51 antibody; 37.51 antibody in vivo depletion

3、Water channel aquaporin 4 is required for T cell receptor mediated lymphocyte activation
Nicosia M, et al. J Leukoc Biol. 2023 Jun 1;113(6):qiad010. doi: 10.1093/jleuko/qiad010. PMID: 36805947
Aquaporins are a family of ubiquitously expressed transmembrane water channels implicated in a broad range of physiological functions. We have previously reported that aquaporin 4 (AQP4) is expressed on T cells and that treatment with a small molecule AQP4 inhibitor significantly delays T cell mediated heart allograft rejection...
Tags: agonist anti-CD28 37.51; 37.51 antibody concentration for T cell culture

4、SEL1L preserves CD8+ T-cell survival and homeostasis by fine-tuning PERK signaling and the IL-15 receptor-mediated mTORC1 axis
Gao Y, et al. Cell Mol Immunol. 2023 Oct;20(10):1232-1250. doi: 10.1038/s41423-023-01078-x. PMID: 37644166
SEL1L-mediated endoplasmic reticulum-associated degradation (ERAD) plays critical roles in controlling protein homeostasis by degrading misfolded or terminal unfolded proteins. However, it remains unclear how SEL1L regulates peripheral T-cell survival and homeostasis...
Tags: soluble vs plate-bound 37.51 antibody; 37.51 antibody titration protocol

5、Sirtuin 5 is Dispensable for CD8+ T Cell Effector and Memory Differentiation
Duan Q, et al. Front Cell Dev Biol. 2021 Dec 13;9:761193. doi: 10.3389/fcell.2021.761193. PMID: 34966740
CD8+ T cell effector and memory differentiation is tightly controlled at multiple levels including transcriptional, metabolic, and epigenetic regulation. Sirtuin 5 (SIRT5) is a protein deacetylase mainly located at mitochondria, but it remains unclear whether SIRT5 plays key roles in regulating CD8 + T cell effector or memory formation...
Tags: 37.51 antibody low endotoxin; Syd Labs Recombinant 37.51 antibody

For more references about anti-mouse CD28 antibody (37.51) please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-mouse CD28 monoclonal antibodies:
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: 37.51)
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: PV-1)

Syd Labs provides the following in vivo grade recombinant anti-human CD28 monoclonal antibodies:
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3)
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3)

Syd Labs provides the following recombinant anti-mouse CD28 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: 37.51) for flow cytometry
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: PV-1) for flow cytometry

Syd Labs provides the following recombinant anti-human CD28 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3) for flow cytometry

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