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Home > Antibodies > Anti-Mouse Antibodies for Flow Cytometry > Anti-mouse CD28 Monoclonal Antibodies for Flow Cytometry

Anti-mouse CD28 Monoclonal Antibodies for Flow Cytometry

T-cell-specific surface glycoprotein CD28; TP44

Catalog No. Product Name Size List Price (US$) Quantity
PA007491.m1 Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: 37.51), Mouse IgG1 Kappa 100 ug 50.00
PA007491.m1 Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: 37.51), Mouse IgG1 Kappa 500 ug 100.00
PA007491.h1Fs Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: 37.51), Human IgG1 Fc Silent Kappa 100 ug 50.00
PA007491.h1Fs Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: 37.51), Human IgG1 Fc Silent Kappa 500 ug 100.00
PA007719.h1Fs-b Biotinylated Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: 37.51), Human IgG1 Fc Silent Kappa 100 ug 75.00
PA007719.h1Fs-b Biotinylated Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: 37.51), Human IgG1 Fc Silent Kappa 500 ug 150.00
Description

PA007491.m1: Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: 37.51), Mouse IgG1 Kappa

Recombinant Mouse IgG1 Monoclonal Antibody. 
Clone: 37.51.
Isotype: Mouse IgG1 Kappa.
Source: The anti-mouse CD28 monoclonal antibody (clone: 37.51) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 37.51 antibody specifically binds to mouse CD28.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007491.h1Fs: Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: 37.51), Human IgG1 Fc Silent Kappa

Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: 37.51.
Isotype: Human IgG1 kappa.
Source: The anti-mouse CD28 monoclonal antibody (clone: 37.51) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 37.51 antibody specifically binds to mouse  CD28.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007719.h1Fs-b: Biotinylated Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: 37.51), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain. 
Clone: 37.51.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated anti-mouse CD28 monoclonal antibody (clone: 37.51) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant 37.51 antibody specifically binds to mouse CD28.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The 37.51 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of anti-mouse CD28 antibody (37.51):

Prevention of lethal acute GVHD with an agonistic CD28 antibody and rapamycin.
Albert, M. H., et al. Blood. 2005 Feb;105(3):1355-61. doi: 10.1182/blood-2004-08-3305. PMID: 15459004
In contrast to blocking costimulation through CD28, administration of agonistic CD28-specific antibody 37.51 partially prevents lethal graft-versus-host disease (GVHD) by selective depletion of alloreactive T cells in mice. ...We hypothesized that combining rapamycin with agonistic CD28 treatment would improve GVHD control by tolerizing a small subset of alloreactive T cells that might escape effects of the CD28-specific antibody. ...Administration of rapamycin plus agonistic CD28 antibodies offers a promising new therapeutic approach to facilitate tolerance after HCT. ...In this study we show that the combination of anti-CD28 mAb and rapamycin has synergistic activity at suboptimal doses and effectively prevents lethal GVHD. ...In further experiments, we investigated whether anti-CD28 mAb is also effective in a very stringent, fully myeloablative GVHD model.
Tags: anti-mouse CD28; function of 37.51 antibody

For more references about anti-mouse CD28 antibody (37.51) please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-mouse CD28 monoclonal antibodies:
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: 37.51)
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: PV-1)

Syd Labs provides the following in vivo grade recombinant anti-human CD28 monoclonal antibodies:
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3)
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3)

Syd Labs provides the following recombinant anti-mouse CD28 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: 37.51) for flow cytometry
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: PV-1) for flow cytometry

Syd Labs provides the following recombinant anti-human CD28 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3) for flow cytometry

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