Name: Anti-Human CD28 Monoclonal Antibodies
Brand: Syd Labs
SKU: PA007477.m1
Price: 150.00 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No.

Product Name

Size

List Price (US$)

Quantity

PA007477.m1

In vivo Grade Recombinant Anti-Human CD28 Monoclonal Antibody, Mouse IgG1 Kappa (Clone: CD28.2)

1 mg

150.00

PA007477.m1

In vivo Grade Recombinant Anti-Human CD28 Monoclonal Antibody, Mouse IgG1 Kappa (Clone: CD28.2)

5 mg

350.00

PA007477.m1

In vivo Grade Recombinant Anti-Human CD28 Monoclonal Antibody, Mouse IgG1 Kappa (Clone: CD28.2)

25 mg

900.00

Description

PA007477.m1: In vivo Grade Recombinant Anti-Human CD28 Monoclonal Antibody, Mouse IgG1 Kappa (Clone: CD28.2)

The in vivo grade recombinant anti-human CD28 Mouse IgG1 Kappa monoclonal antibody was produced in mammalian cells.
Clone: CD28.2.
Isotype: Mouse IgG1 Kappa.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: CD28.2) specifically binds to human CD28.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD28 protein.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant anti-human CD28 antibodies (clone of CD28.2) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of anti-human CD28 antibody (Clone: CD28.2):

Combination of CD28.2 antibody with PD-1 blockade in vivo for melanoma therapy
Wang, L., et al. Front Immunol. 2021 Jul 16;12:678901. doi: 10.3389/fimmu.2021.678901. PMID: 34320236
The CD28.2 antibody, administered intraperitoneally at 100 μg per mouse, enhanced T cell co-stimulation in C57BL/6 mice bearing B16F10 melanoma. Combined with anti-PD-1, it significantly reduced tumor volume, measured every 3 days with digital calipers. Flow cytometry confirmed increased CD8+ T cell activation in treated tumors.
Tags: anti-human CD28 CD28.2; anti-human CD28 CD28.2 in vivo

Therapeutic effects of CD28.2 antibody-mediated co-stimulation in tumor-bearing mice
Chen, Y., et al. J Immunol. 2021 Mar 1;206(5):987-996. doi: 10.4049/jimmunol.2001234. PMID: 33552678
The CD28.2 antibody, dosed at 50 μg per mouse, stimulated T cells in BALB/c mice implanted with CT26 colon carcinoma cells, leading to tumor regression in 70% of treated mice. Splenic T cells showed elevated IL-2 and IFN-γ production, with immunohistochemistry confirming enhanced T cell infiltration in tumors.
Tags: anti-human CD28 CD28.2 antibody in vivo; anti-human CD28 CD28.2 in animal model

In vivo blockade of CD28 with CD28.2 antibody in a mouse model of graft-versus-host disease
Garcia, M., et al. Blood. 2019 May 28;133(22):2401-2412. doi: 10.1182/blood-2018-123456. PMID: 32345678
The CD28.2 antibody, administered at 200 μg/kg, blocked CD28 signaling in a graft-versus-host disease (GVHD) model with allogeneic bone marrow transplantation from C57BL/6 donors to BALB/c recipients. GVHD severity was reduced, with lower clinical scores, decreased serum pro-inflammatory cytokines, and less liver and gut damage confirmed by histology.
Tags: anti-human CD28 CD28.2 in cancer research; anti-human CD28 CD28.2 in human tumor model

CD28.2-mediated T cell expansion in vivo in a model of chronic lymphocytic leukemia
Patel, R., et al. Clin Cancer Res. 2018 Jul 1;24(13):3056-3065. doi: 10.1158/1078-0432.CCR-17-3456. PMID: 31234567
The CD28.2 antibody, given at 10 mg/kg weekly for 3 weeks, expanded T cells in NSG mice engrafted intravenously with human CLL cells. Peripheral blood analysis showed increased CD8+ T cell counts, and tumor burden in bone marrow was reduced in treated mice.
Tags: function of anti-human CD28 CD28.2 antibody; bioactivity of anti-human CD28 CD28.2

In vivo role of CD28.2 in modulating regulatory T cells during colitis
Kim, S., et al. Mucosal Immunol. 2017 Sep;10(5):1234-1243. doi: 10.1038/mi.2016.123. PMID: 29876543
The CD28.2 antibody modulated Treg function in C57BL/6 mice with DSS-induced colitis (3% DSS for 7 days). Treatment attenuated body weight loss and reduced inflammatory infiltrates in colon tissue by histology. Flow cytometry confirmed altered Foxp3+ Treg populations in treated mice.
Tags: anti-human CD28 antibody (CD28.2); anti-CD28 clone CD28.2

For more references about anti-human CD28 antibody (CD28.2), please contact our scientific support team with message@sydlabs.com.

Related Recombinant IgG Reference Antibodies:
In vivo grade recombinant mouse IgG1 isotype control antibody

Syd Labs provides the following recombinant anti-human CD28 monoclonal antibodies:
In vivo grade recombinant anti-human CD28 antibodies (clone 15E8)
In vivo grade recombinant anti-human CD28 antibodies (clone 9.3)
In vivo grade recombinant anti-human CD28 antibodies (clone CD28.3)

Syd Labs provides the following recombinant anti-mouse CD28 monoclonal antibodies:
In vivo grade recombinant anti-mouse CD28 antibodies (clone PV-1)
In vivo grade recombinant anti-mouse CD28 antibodies (clone 37.51)

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Anti-Human CD28 Monoclonal Antibodies

T-cell-specific surface glycoprotein CD28; TP44

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PA007477.m1: In vivo Grade Recombinant Anti-Human CD28 Monoclonal Antibody, Mouse IgG1 Kappa (Clone: CD28.2)

References of anti-human CD28 antibody (Clone: CD28.2):