Anti-Mouse CD28 Monoclonal Antibodies

PA007463.m2a: In vivo Grade Recombinant Anti-Mouse CD28 Monoclonal Antibody, Mouse IgG2a Kappa (Clone: PV-1)

The in vivo grade recombinant anti-mouse CD28 Mouse IgG2a Kappa monoclonal antibody was produced in mammalian cells. 
Clone: PV-1.
Isotype: Mouse IgG2a Kappa.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: PV-1) specifically binds to mouse CD28.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD28 protein. 
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007463.hs: In vivo Grade Recombinant Anti-Mouse CD28 Monoclonal Antibody, Armenian Hamster IgG Kappa (Clone: PV-1)

The in vivo grade recombinant anti-mouse CD28 Armenian Hamster IgG Kappa monoclonal antibody was produced in mammalian cells. 
Clone: PV-1.
Isotype: Armenian Hamster IgG Kappa.
Specificity/Sensitivity: The in vivo grade recombinant hamster monoclonal antibody (clone: PV-1) specifically binds to mouse CD28.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD28 protein. 
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant anti-mouse CD28 antibodies (clone of PV-1) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-Mouse CD28 Monoclonal Antibody:

CXCL13 antibody for the treatment of autoimmune disorders.
Klimatcheva, E., et al. BMC Immunol. 2015 Feb 12;16(1):6. doi: 10.1186/s12865-015-0068-1. PMID: 25879435
The cells were incubated at 37°C for 4 days after which the cells were collected, washed and cultured in media containing 2 μg/ml of each anti-muCD3e and anti-muCD28 antibody for 3 days. For the adoptive transfer, the mice were injected i.p. with 2×106 cells in 100 μl PBS. Starting on the day of the adoptive transfer, the animals were given bi-weekly i.p. injections of 30 mg/kg of the antibodies for the total of 4 weeks. ...Antibodies: rat anti-mouse MAb 470 (R&D Systems; MAB470); mouse anti-human MAb 801 (R&D Systems; MAB801); goat anti-human AF801 (R&D Systems; AF801); goat anti-mouse AF470 (R&D Systems; AF470); anti-human CXCR5-Alexa 647 (BD Pharmingen; 558113); anti-human CXCR4-APC (R&D Systems; FAB170A); donkey anti-mouse IgG-HRP (Jackson ImmunoResearch; 715-035-150); donkey anti-rat IgG-HRP (Jackson ImmunoResearch; 712-035-150); B220-FITC (BD Biosciences; 553087); CD21/35-APC (BD Biosciences; 558658); CD23-PE (BD Biosciences; 553139); anti-human CD32 (Biolegend; 303202); anti-murine IL-4 (clone 11B11; BioXcell, BE0055); anti-murine CD3e (clone 145-2C11; BioXcell; BE0001-1); anti-murine CD28 (clone PV-1; BioXcell; BE0015-5); anti-murine IFN-γ (clone XMG1.2; BioXcell; BE0055). ...The V genes were isolated from 3D2 hybridoma using standard methods. The VH gene was cloned into a mammalian expression vector that contained the human gamma 1 heavy chain gene, creating a full-length chimeric heavy chain. ...The VK was cloned into a mammalian expression vector that contained the human Kappa constant gene, creating a full-length chimeric light chain. ...Generation of human anti-CXCL13 antibody through humanization of chimeric antibody was accomplished as follows.
Tags: anti-mouse CD28 PV-1; anti-mouse CD28 PV-1 antibody

IL-27 and IL-12 oppose pro-inflammatory IL-23 in CD4+ T cells by inducing Blimp1.
Heinemann, C., et al. Nat Commun. 2014 May 6:5:3770. doi: 10.1038/ncomms4770. PMID: 24796719
Naive CD4+ T cells were polyclonally stimulated for 3 days with plate-bound antibody to CD3 and soluble antibody to CD28 in the absence of exogenous cytokines (Th0), or differentiated into Tr-1 cells (100 ng ml−1 IL-27 plus 0.5 ng ml−1 TGF-β) or Th17 cells (50 ng ml−1 IL-6 plus 0.5 ng ml−1 TGF-β) for microarray analysis (Affymetrix). ...Naive CD4+ T cells were differentiated by activation with plate-bound anti-CD3 and soluble anti-CD28 with IL-27 alone (in the presence of a blocking antibody to TGF-β) or with IL-27 in the presence of increasing concentrations of TGF-β. Quantification of the indicated messenger RNAs was performed by quantitative RT–PCR on day 3. Mean±s.d., n=3, representative of three experiments. ...For in vitro T-cell differentiation, magnetic bead-purified CD4+CD62L+ (Miltenyi) or flow cytometrically purified CD4+CD44−CD25− T cells were stimulated for 3 days with 4 μg ml−1 plate-bound antibody to CD3 (BioXcell, 145-2C11) and 2 μg ml−1 soluble antibody to CD28 (BioXcell, PV-1). ...Recombinant cytokines were obtained from R&D systems. If not otherwise indicated, Th1 cells were differentiated in the presence of 10 ng ml−1 IL-12 and 10 μg ml−1 anti-IL-4 (11B11, BioXcell), Th17 cells were differentiated in the presence of 0.5 ng ml−1 TGF-β1 and 50 ng ml−1 IL-6 and Tr-1 cells were differentiated with 0.5 ng ml−1 TGF-β1 and 100 ng ml−1 IL-27. ...Statistical evaluations of cell frequency measurements, cell numbers, messenger RNA and protein levels were performed with the unpaired Student’s t-test when two populations were compared.
Tags: anti-mouse CD28 PV-1 in vivo; anti-mouse CD28 PV-1 mAb in vivo

Ezrin and moesin are required for efficient T cell adhesion and homing to lymphoid organs.
Chen, E. J. H., et al. PLoS One. 2013 Feb 28;8(2):e52368. doi: 10.1371/journal.pone.0052368. PMID: 23468835
T cells were stimulated in 24-well plates coated with 1 ug/mL anti-CD28 (PV1) and anti-CD3 (2C11) (both from BioXCell) for three days and maintained in DMEM supplemented with 5% FBS, penicillin, streptomycin, glutaMAX, Hepes, NEAA and β-mercaptoethanol at 37°C, 10% CO2. ...SiControl #2 was used as a control. To produce ezrin and/or moesin deficient murine CD4+ T cell blasts, freshly isolated ezrinflox/flox or ezrinflox/flox:CD4cre T cells were stimulated as described above and removed from stimulation for two days. ...Cells were prepared and transferred to recipient mice as described for in vivo homing assays. ...Adhesion assays were performed in triplicate as previously described. ...To assay diapedesis in the absence of shear flow, 3.5×105 3B-11 endothelial cells were seeded in 35 mm tissue culture dishes (Corning) and cultured overnight to form a confluent monolayer.
Tags: anti-mouse CD28 PV-1 antibody in animal model; anti-mouse CD28 PV-1 in cancer research

IL-9 as a mediator of Th17-driven inflammatory disease.
Nowak, E. C., et al. J Exp Med. 2009 Aug 3;206(8):1653-60. doi: 10.1084/jem.20090246. PMID: 19596803
Th17 cells were generated with 2.5 ng/ml TGF-β, 20 ng/ml IL-6, 10 µg/ml αIFN-γ, and 10 µg/ml αIL-4 using 5 µg/ml of plate-bound CD3 (145-2C11; BioXCell) and CD28 (PV-1; BioXCell), respectively. ...Cells were washed, counted, and restimulated in the presence of αCD3/αCD28 for 24 h before supernatants were collected for ELISA. ...The frequency of cytokine-producing cells by FACS was used to calculate the absolute numbers of cells shown. *, P < 0.01 by the Student’s t test. ...Representative FACS plots are shown of WT and KO mice. Absolute numbers of MCs in lymph nodes were determined for WT naive (n = 5), KO naive (n = 5), WT EAE (n = 12), and KO EAE (n = 14) mice pooled from four independent experiments. ...Results show means ± SD of two independent experiments, and a Student’s t test was performed to compare the samples.
Tags: function of anti-mouse CD28 PV-1 mAb; bioactivity of anti-mouse CD28 PV-1 mAb

For more references about Anti-Mouse CD28 Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.

Related Recombinant IgG Reference Antibodies:
In vivo grade recombinant mouse IgG2a isotype control antibody
In vivo grade recombinant hamster IgG1 isotype control antibody
In vivo grade recombinant hamster IgG2 isotype control antibody

Syd Labs provides the following recombinant anti-human CD28 monoclonal antibodies:
In vivo grade recombinant anti-human CD28 monoclonal antibodies (clone 15E8)
In vivo grade recombinant anti-human CD28 monoclonal antibodies (clone 9.3)
In vivo grade recombinant anti-human CD28 monoclonal antibodies (clone CD28.3)

Syd Labs provides the following recombinant anti-mouse CD28 monoclonal antibodies:
In vivo grade recombinant anti-mouse CD28 monoclonal antibodies (clone PV-1)
In vivo grade recombinant anti-mouse CD28 monoclonal antibodies (clone 37.51