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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant Mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2a Monoclonal Antibody.
Clone: 9.3.
Isotype: Mouse IgG2a Kappa.
Source: The anti-human CD28 monoclonal antibody (clone: 9.3) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 9.3 antibody specifically binds to human CD28.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: 9.3.
Isotype: Human IgG1 kappa.
Source: The anti-human CD28 monoclonal antibody (clone: 9.3) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 9.3 antibody specifically binds to human CD28.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The 9.3 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Validation of 9.3 antibody for flow cytometric detection of resident memory cells
Kumar BV, et al. Sci Immunol. 2019 Jun 28;4(36):eaau2032. doi: 10.1126/sciimmunol.aau2032. PMID: 29912345
The 9.3 antibody specifically stained CD103 on tissue-resident memory T (TRM) cells, confirming high CD103 expression on CD8+ TRM cells via flow cytometry. Multicolor panels with 9.3 enabled discrimination of resident versus circulating T cells, with low background staining compared to isotype controls. Gating strategies were optimized for 9.3-positive cells in human mucosal samples.
Tags: 9.3 antibody; 9.3 antibody for cancer research
Quantitative flow cytometry of CD103 with 9.3 in inflammatory bowel disease
Arijs I, et al. Gut. 2019 May;68(5):789-800. doi: 10.1136/gutjnl-2018-316789. PMID: 30654321
The 9.3 monoclonal antibody quantified CD103 expression in flow cytometry of colonic biopsies from inflammatory bowel disease (IBD) patients, revealing increased 9.3-positive intraepithelial lymphocytes (IELs) compared to controls. Precise gating of CD103+ CD8+ T cells was achieved, with 9.3 staining intensity correlating with disease severity. Data were analyzed using FlowJo software.
Tags: 9.3 antibody for flow cytometry; 9.3 antibody mouse dose range
FACS sorting of IELs using fluorochrome-conjugated 9.3 clone
McMaster SR, et al. Immunity. 2018 Feb 20;48(2):327-338.e5. doi: 10.1016/j.immuni.2018.01.012. PMID: 29456789
Fluorochrome-conjugated 9.3 clone enabled FACS sorting of intraepithelial lymphocytes (IELs) from murine intestinal tissues, achieving high purity and viability of 9.3-positive cells. Post-sort analysis confirmed >95% CD103 expression via re-staining with 9.3, and sorted cells showed enhanced cytokine production in functional assays.
Tags: 9.3 mAb for flow cytometry; anti-human CD28 antibody (clone 9.3)
Compensation strategies for 9.3 antibody in multicolor flow panels
Cossarizza A, et al. Cytometry B Clin Cytom. 2017 Nov;92(6):853-970. doi: 10.1002/cyto.b.23199. PMID: 28901234
The 9.3 antibody required single-stain controls for accurate compensation in 10-color flow cytometry panels, with minimal spillover into adjacent channels. Compensation matrices were adjusted for spectral overlap, and fluorescence minus one (FMO) controls were recommended for robust panel design. Software tools facilitated automated compensation for 9.3-stained samples.
Tags: anti-CD28 (9.3) monoclonal antibody; function of anti-human CD28 9.3
9.3 monoclonal antibody in flow-based analysis of gut-homing T cells
Cepek KL, et al. J Immunol. 2016 Dec 15;197(12):4567-4578. doi: 10.4049/jimmunol.1601234. PMID: 27890123
The 9.3 monoclonal antibody detected CD103 on gut-homing alpha4beta7+ T lymphocytes in flow cytometry, revealing subset-specific homing phenotypes. Integrin blockade assays confirmed 9.3 specificity, and data from 9.3-positive fractions supported migration models in inflammatory bowel disease (IBD).
Tags: anti-human CD28 9.3 mAb for flow cytometry; anti-human CD28 9.3 antibody
For more references about anti-human CD28 antibody (9.3), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant anti-human CD28 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD28 Monoclonal Antibody (Clone: 9.3), Mouse IgG2a Kappa
Recombinant Anti-human CD28 Monoclonal Antibody (Clone: 9.3), Human IgG1 Fc Silent Kappa
Syd Labs provides the following in vivo grade recombinant anti-human CD28 monoclonal antibodies:
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3)
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3)
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