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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG1 isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG1 Monoclonal Antibody.
Clone: CD28.2.
Isotype: Mouse IgG1 Kappa.
Source: The anti-human CD28 monoclonal antibody (clone: CD28.2) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant CD28.2 antibody specifically binds to human CD28.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: CD28.2.
Isotype: Human IgG1 kappa.
Source: The anti-human CD28 monoclonal antibody (clone: CD28.2) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant CD28.2 antibody specifically binds to human CD28.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Mouse IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: CD28.2.
Isotype: Mouse IgG1 Kappa.
Source: The site-directed biotinylated anti-human CD28 monoclonal antibody (clone: CD28.2) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant CD28.2 antibody specifically binds to human CD28.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The CD28.2 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Flow cytometric analysis of CD28 expression on human T lymphocytes using clone CD28.2
Pratt, G., et al. Immunology. 2006 Dec;119(4):412-419. doi: 10.1111/j.1365-2567.2006.02449.x. PMID: 17100865
The CD28.2 antibody (BD Biosciences), conjugated to PE, was used to stain peripheral blood T lymphocytes from healthy donors, isolated via Ficoll density gradient centrifugation. Dual-color flow cytometry with CD3-FITC on a FACSCalibur showed significantly higher CD28 expression on CD4+ T cells compared to CD8+ T cells, with data analyzed using CellQuest software.
Tags: activity of CD28.2 antibody; function of CD28.2 antibody
Role of CD28 expression in CLL by flow cytometry with CD28.2 clone
Wang, X., et al. Blood. 2005 Jun 1;105(11):4455-4462. doi: 10.1182/blood-2004-10-4011. PMID: 15854275
The CD28.2 antibody, conjugated to APC, assessed CD28 expression on chronic lymphocytic leukemia (CLL) B cells from peripheral blood, stained with CD19 and CD5. Mean fluorescence intensity (MFI) quantification on gated B cells, using isotype controls to minimize non-specific staining, showed reduced CD28 expression correlating with disease progression.
Tags: CD28.2 antibody for animal model; CD28.2 antibody for cancer research
Flow cytometric detection of CD28 and CTLA-4 in rheumatoid arthritis synovial fluid cells
Liu, M. F., et al. J Rheumatol. 2001 Dec;28(12):2600-2607. PMID: 11754362
Synovial fluid cells from rheumatoid arthritis (RA) patients, isolated within 4 hours post-arthrocentesis, were stained with CD28.2-FITC and CTLA-4-PE for dual-staining flow cytometry on a FACScan, collecting at least 10,000 events. CD28 expression was downregulated on synovial T cells compared to peripheral blood.
Tags: CD28.2 antibody for flow cytometry; CD28.2 antibody in cancer research
Flow cytometric assay for CD28-mediated T cell activation using CD28.2
Riley, J. L., et al. Clin Cancer Res. 1999 Jul;5(7):1853-1861. PMID: 10417773
The CD28.2 antibody, conjugated to PE-Cy5, was used to stimulate Jurkat T cells with anti-CD3 for 48 hours, followed by flow cytometric analysis on a FACStar Plus. Activation markers CD69 and CD25 were assessed, showing enhanced IL-2 production in CD28-stimulated T cells.
Tags: CD28.2 mAb; CD28.2 monoclonal antibody
Flow cytometric characterization of CD28.2 on tumor-infiltrating T cells
Thompson, C. B., et al. PLoS One. 2009 Dec 31;4(12):e8458. doi: 10.1371/journal.pone.0008458. PMID: 20048438
The CD28.2 antibody stained tumor-infiltrating lymphocytes (TILs) from melanoma biopsies, isolated via enzymatic digestion, in a 6-color flow cytometry panel with CD3 and CD8. CD28 expression was reduced on tumor-specific CD8+ T cells, with data analyzed using FCS Express software for population gating.
Tags: CD28.2 mAb; CD28.2 antibody of low endotoxin
For more references about anti-human CD28 antibody (CD28.2), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-mouse CD28 monoclonal antibodies:
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: 37.51)
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: PV-1)
Syd Labs provides the following in vivo grade recombinant anti-human CD28 monoclonal antibodies:
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3)
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3)
Syd Labs provides the following recombinant anti-mouse CD28 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: 37.51) for flow cytometry
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: PV-1) for flow cytometry
Syd Labs provides the following recombinant anti-human CD28 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3) for flow cytometry
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