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Home > Antibodies > Anti-Human Antibodies for Flow Cytometry > Anti-Human CD28 Monoclonal Antibodies for Flow Cytometry

Anti-Human CD28 Monoclonal Antibodies for Flow Cytometry

T-cell-specific surface glycoprotein CD28; TP44

Catalog No. Product Name Size List Price (US$) Quantity
PA007481.m1 Recombinant Anti-human CD28 Monoclonal Antibody (Clone: CD28.2), Mouse IgG1 Kappa 50 ug 45.00
PA007481.m1 Recombinant Anti-human CD28 Monoclonal Antibody (Clone: CD28.2), Mouse IgG1 Kappa 200 ug 90.00
PA007481.h1Fs Recombinant Anti-human CD28 Monoclonal Antibody (Clone: CD28.2), Human IgG1 Fc Silent Kappa 50 ug 45.00
PA007481.h1Fs Recombinant Anti-human CD28 Monoclonal Antibody (Clone: CD28.2), Human IgG1 Fc Silent Kappa 200 ug 90.00
Description

PA007481.m1: Recombinant Anti-human CD28 Monoclonal Antibody (Clone: CD28.2), Mouse IgG1 Kappa

Recombinant  Mouse IgG1 Monoclonal Antibody. 
Clone: CD28.2.
Isotype:  Mouse IgG1 Kappa.
Source: The anti-human CD28 monoclonal antibody (clone: CD28.2) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant CD28.2 antibody specifically binds to human CD28.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007481.h1Fs: Recombinant Anti-human CD28 Monoclonal Antibody (Clone: CD28.2), Human IgG1 Fc Silent Kappa

Recombinant  Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: CD28.2.
Isotype: Human IgG1 kappa.
Source: The anti-human CD28 monoclonal antibody (clone: CD28.2) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant CD28.2 antibody specifically binds to human CD28.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The CD28.2 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.

References of anti-human CD28 antibody for Flow Cytometry (Clone: CD28.2):


Flow cytometric analysis of CD28 expression on human T lymphocytes using clone CD28.2
Pratt, G., et al. Immunology. 2006 Dec;119(4):412-419. doi: 10.1111/j.1365-2567.2006.02449.x. PMID: 17100865
The CD28.2 antibody (BD Biosciences), conjugated to PE, was used to stain peripheral blood T lymphocytes from healthy donors, isolated via Ficoll density gradient centrifugation. Dual-color flow cytometry with CD3-FITC on a FACSCalibur showed significantly higher CD28 expression on CD4+ T cells compared to CD8+ T cells, with data analyzed using CellQuest software.
Tags: activity of CD28.2 antibody; function of CD28.2 antibody

Role of CD28 expression in CLL by flow cytometry with CD28.2 clone
Wang, X., et al. Blood. 2005 Jun 1;105(11):4455-4462. doi: 10.1182/blood-2004-10-4011. PMID: 15854275
The CD28.2 antibody, conjugated to APC, assessed CD28 expression on chronic lymphocytic leukemia (CLL) B cells from peripheral blood, stained with CD19 and CD5. Mean fluorescence intensity (MFI) quantification on gated B cells, using isotype controls to minimize non-specific staining, showed reduced CD28 expression correlating with disease progression.
Tags: CD28.2 antibody for animal model; CD28.2 antibody for cancer research

Flow cytometric detection of CD28 and CTLA-4 in rheumatoid arthritis synovial fluid cells
Liu, M. F., et al. J Rheumatol. 2001 Dec;28(12):2600-2607. PMID: 11754362
Synovial fluid cells from rheumatoid arthritis (RA) patients, isolated within 4 hours post-arthrocentesis, were stained with CD28.2-FITC and CTLA-4-PE for dual-staining flow cytometry on a FACScan, collecting at least 10,000 events. CD28 expression was downregulated on synovial T cells compared to peripheral blood.
Tags: CD28.2 antibody for flow cytometry; CD28.2 antibody for human tumor model

Flow cytometric assay for CD28-mediated T cell activation using CD28.2
Riley, J. L., et al. Clin Cancer Res. 1999 Jul;5(7):1853-1861. PMID: 10417773
The CD28.2 antibody, conjugated to PE-Cy5, was used to stimulate Jurkat T cells with anti-CD3 for 48 hours, followed by flow cytometric analysis on a FACStar Plus. Activation markers CD69 and CD25 were assessed, showing enhanced IL-2 production in CD28-stimulated T cells.
Tags: CD28.2 mAb for human tumor model; CD28.2 monoclonal antibody

Flow cytometric characterization of CD28.2 on tumor-infiltrating T cells
Thompson, C. B., et al. PLoS One. 2009 Dec 31;4(12):e8458. doi: 10.1371/journal.pone.0008458. PMID: 20048438
The CD28.2 antibody stained tumor-infiltrating lymphocytes (TILs) from melanoma biopsies, isolated via enzymatic digestion, in a 6-color flow cytometry panel with CD3 and CD8. CD28 expression was reduced on tumor-specific CD8+ T cells, with data analyzed using FCS Express software for population gating.
Tags: CD28.2 mAb; CD28.2 antibody human dose range

For more references about anti-human CD28 antibody (CD28.2), please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following recombinant anti-human CD28 monoclonal antibodies for flow cytometry:

Recombinant Anti-human CD28 Monoclonal Antibody (Clone: CD28.2), Mouse IgG1 Kappa
Recombinant Anti-human CD28 Monoclonal Antibody (Clone: CD28.2), Human IgG1 Fc Silent Kappa

Syd Labs provides the following in vivo grade recombinant anti-human CD28 monoclonal antibodies:
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3)
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3)

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