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Home > Antibodies > Anti-Mouse Antibodies for Flow Cytometry > Anti-mouse CD28 Monoclonal Antibodies for Flow Cytometry

Anti-mouse CD28 Monoclonal Antibodies for Flow Cytometry

T-cell-specific surface glycoprotein CD28; TP44

Catalog No. Product Name Size List Price (US$) Quantity
PA007490.m2a Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: PV-1), Mouse IgG2a Kappa 500 ug 75.00
PA007490.h1Fs Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: PV-1), Human IgG1 Fc Silent Kappa 500 ug 75.00
Description

PA007490.m2a: Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: PV-1), Mouse IgG2a Kappa

Recombinant Mouse IgG2a Monoclonal Antibody. 
Clone: PV-1.
Isotype:  Mouse IgG2a Kappa.
Source: The anti-mouse CD28 monoclonal antibody (clone: PV-1) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant PV-1 antibody specifically binds to mouse CD28.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007490.h1Fs: Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: PV-1), Human IgG1 Fc Silent Kappa

Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: PV-1.
Isotype: Human IgG1 kappa.
Source: The anti-mouse CD28 monoclonal antibody (clone: PV-1) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant PV-1 antibody specifically binds to mouse  CD28.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The PV-1 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.

References of anti-mouse CD28 antibody (PV-1)


Comprehensive Phenotyping of Endothelial Cells Using Flow Cytometry 2: Human.
Duan Y, et al. Cytometry A. 2021 Mar;99(3):257-265. doi: 10.1002/cyto.a.24293. PMID: 33211372
The anti-human PLVAP/PV-1 antibody used was clone MECA-32 (Santa Cruz Biotechnology, sc-19603). Human endothelial cells were isolated from fresh tissue samples. Flow cytometry was performed using a BD FACSCanto II. Cells were stained for CD31, CD144, and PLVAP to confirm endothelial identity. Data analysis was conducted with FlowJo software.
Tags: activity of PV-1 mAb; function of PV-1 mAb

Comprehensive Phenotyping of Endothelial Cells Using Flow Cytometry 1: Murine.
Duan Y, et al. Cytometry A. 2021 Mar;99(3):247-256. doi: 10.1002/cyto.a.24292. PMID: 33211373
For mouse lung endothelial cells (MLEC), single-cell suspensions were stained with rat anti-mouse PLVAP/PV-1 antibody (clone MECA-32, BioLegend, #120506) and analyzed by flow cytometry. Mouse tissues were digested using collagenase. Flow cytometry was performed on a BD LSRFortessa. Endothelial cells were gated as CD45-CD31+PLVAP+. Data were analyzed using FCS Express software.
Tags: PV-1 antibody for animal model; PV-1 antibody for flow cytometry

Defining the role of pulmonary endothelial cell heterogeneity in the response to viral infection.
Niethamer TK, et al. eLife. 2020 Apr 23;9:e53072. doi: 10.7554/eLife.53072. PMID: 32332020
Primary antibodies used in flow cytometry included rat anti-mouse CD31 (clone 390, eBioscience), rat anti-mouse CD45 (clone 30-F11, BioLegend), and rat anti-mouse PV-1 (clone MECA-32, BioLegend). Mice were infected intranasally with influenza virus. Lung tissues were processed into single-cell suspensions. Flow cytometry was conducted using a BD LSR II. Endothelial cell subsets were identified based on CD31 and PV-1 expression.
Tags: PV-1 antibody mouse dose range; PV-1 mAb for flow cytometry

The diaphragms of fenestrated endothelia: gatekeepers of vascular permeability and blood composition.
Stan RV, et al. Dev Cell. 2012 Dec 11;23(6):1203-18. doi: 10.1016/j.devcel.2012.11.003. PMID: 23178176
PV-1, a marker of fenestrated endothelium, was stained using rat anti-mouse PV-1 (clone MECA-32) in flow cytometry experiments. Fenestrated endothelial cells were isolated from mouse kidneys. Cells were stained for CD31, VE-cadherin, and PV-1. Flow cytometry was performed on a FACSCalibur. Permeability assays were conducted in vivo using tracer dyes.
Tags: PV-1 mAb for mouse tumor model; PV-1 monoclonal antibody

Caveolae, fenestrae and transendothelial channels retain PV1 on the surface of endothelial cells.
Tkachenko E, et al. PLoS One. 2012;7(3):e32655. doi: 10.1371/journal.pone.0032655. PMID: 22431958
To verify the presence of PV1 on the surface of endothelial cells, cells were stained with a rat monoclonal antibody against PV1 (clone MECA-32; BD Pharmingen) and analyzed by flow cytometry. Endothelial cells were isolated from mouse lungs. Cells were stained with anti-CD31 and anti-PV1 antibodies. Flow cytometry was performed using a BD FACSAria. Surface expression was quantified relative to isotype controls.
Tags: purified anti-mouse CD28 (PV-1); PV-1 anti-mouse CD28 antibody

For more references about anti-mouse CD28 antibody (PV-1), please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-mouse CD28 monoclonal antibodies:
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: PV-1)

Syd Labs provides the following in vivo grade recombinant anti-human CD28 monoclonal antibodies:
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3)
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3)

Syd Labs provides the following recombinant anti-human CD28 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3) for flow cytometry

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