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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
|---|
Recombinant Mouse IgG2a isotype controls and Recombinant Human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2a Monoclonal Antibody.
Clone: PV-1.
Isotype: Mouse IgG2a Kappa.
Source: The anti-mouse CD28 monoclonal antibody (clone: PV-1) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant PV-1 antibody specifically binds to mouse CD28.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: PV-1.
Isotype: Human IgG1 kappa.
Source: The anti-mouse CD28 monoclonal antibody (clone: PV-1) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant PV-1 antibody specifically binds to mouse CD28.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The PV-1 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Comprehensive Phenotyping of Endothelial Cells Using Flow Cytometry 2: Human.
Duan Y, et al. Cytometry A. 2021 Mar;99(3):257-265. doi: 10.1002/cyto.a.24293. PMID: 33211372
The anti-human PLVAP/PV-1 antibody used was clone MECA-32 (Santa Cruz Biotechnology, sc-19603). Human endothelial cells were isolated from fresh tissue samples. Flow cytometry was performed using a BD FACSCanto II. Cells were stained for CD31, CD144, and PLVAP to confirm endothelial identity. Data analysis was conducted with FlowJo software.
Tags: activity of PV-1 mAb; function of PV-1 mAb
Comprehensive Phenotyping of Endothelial Cells Using Flow Cytometry 1: Murine.
Duan Y, et al. Cytometry A. 2021 Mar;99(3):247-256. doi: 10.1002/cyto.a.24292. PMID: 33211373
For mouse lung endothelial cells (MLEC), single-cell suspensions were stained with rat anti-mouse PLVAP/PV-1 antibody (clone MECA-32, BioLegend, #120506) and analyzed by flow cytometry. Mouse tissues were digested using collagenase. Flow cytometry was performed on a BD LSRFortessa. Endothelial cells were gated as CD45-CD31+PLVAP+. Data were analyzed using FCS Express software.
Tags: PV-1 antibody for animal model; PV-1 antibody for flow cytometry
Defining the role of pulmonary endothelial cell heterogeneity in the response to viral infection.
Niethamer TK, et al. eLife. 2020 Apr 23;9:e53072. doi: 10.7554/eLife.53072. PMID: 32332020
Primary antibodies used in flow cytometry included rat anti-mouse CD31 (clone 390, eBioscience), rat anti-mouse CD45 (clone 30-F11, BioLegend), and rat anti-mouse PV-1 (clone MECA-32, BioLegend). Mice were infected intranasally with influenza virus. Lung tissues were processed into single-cell suspensions. Flow cytometry was conducted using a BD LSR II. Endothelial cell subsets were identified based on CD31 and PV-1 expression.
Tags: PV-1 antibody mouse dose range; PV-1 mAb for flow cytometry
The diaphragms of fenestrated endothelia: gatekeepers of vascular permeability and blood composition.
Stan RV, et al. Dev Cell. 2012 Dec 11;23(6):1203-18. doi: 10.1016/j.devcel.2012.11.003. PMID: 23178176
PV-1, a marker of fenestrated endothelium, was stained using rat anti-mouse PV-1 (clone MECA-32) in flow cytometry experiments. Fenestrated endothelial cells were isolated from mouse kidneys. Cells were stained for CD31, VE-cadherin, and PV-1. Flow cytometry was performed on a FACSCalibur. Permeability assays were conducted in vivo using tracer dyes.
Tags: PV-1 mAb for mouse tumor model; PV-1 monoclonal antibody
Caveolae, fenestrae and transendothelial channels retain PV1 on the surface of endothelial cells.
Tkachenko E, et al. PLoS One. 2012;7(3):e32655. doi: 10.1371/journal.pone.0032655. PMID: 22431958
To verify the presence of PV1 on the surface of endothelial cells, cells were stained with a rat monoclonal antibody against PV1 (clone MECA-32; BD Pharmingen) and analyzed by flow cytometry. Endothelial cells were isolated from mouse lungs. Cells were stained with anti-CD31 and anti-PV1 antibodies. Flow cytometry was performed using a BD FACSAria. Surface expression was quantified relative to isotype controls.
Tags: purified anti-mouse CD28 (PV-1); PV-1 anti-mouse CD28 antibody
For more references about anti-mouse CD28 antibody (PV-1), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-mouse CD28 monoclonal antibodies:
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: PV-1)
Syd Labs provides the following in vivo grade recombinant anti-human CD28 monoclonal antibodies:
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3)
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3)
Syd Labs provides the following recombinant anti-human CD28 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3) for flow cytometry
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