Anti-Human CD28 Monoclonal Antibodies for Flow Cytometry

PA007469.m2a: Recombinant Anti-human CD28 Monoclonal Antibody (Clone: CD28.3), Mouse IgG2a Kappa

Recombinant  Mouse IgG2a Monoclonal Antibody. 
Clone: CD28.3.
Isotype:  Mouse IgG2a Kappa.
Source: The anti-human CD28 monoclonal antibody (clone: CD28.3) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant CD28.3 antibody specifically binds to human CD28.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007469.h1Fs: Recombinant Anti-human CD28 Monoclonal Antibody (Clone: CD28.3), Human IgG1 Fc Silent Kappa

Recombinant  Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: CD28.3.
Isotype: Human IgG1 kappa.
Source: The anti-human CD28 monoclonal antibody (clone: CD28.3) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant CD28.3 antibody specifically binds to human CD28.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007750.m2a-b: Biotinylated Recombinant Anti-human CD28 Monoclonal Antibody (Clone: CD28.3), Mouse IgG2a Kappa

Recombinant  Mouse IgG2a Monoclonal Antibody with site-directed  biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: CD28.3.
Isotype:  Mouse IgG2a Kappa.
Source: The site-directed biotinylated anti-human CD28 monoclonal antibody (clone: CD28.3) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant CD28.3 antibody specifically binds to human CD28.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The CD28.3 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of anti-human CD28 antibody for Flow Cytometry (Clone: CD28.3):

Flow cytometric analysis of CD28 expression on human T lymphocytes using clone CD28.3
Sutherland DR, et al. Cytometry. 2000 Jan 1;40(1):1-10. doi: 10.1002/(SICI)1097-0320(20000101)40:1<1::AID-CYTO1>3.0.CO;2-#. PMID: 10653588
The CD28.3 monoclonal antibody was used to stain CD28 on resting and activated T cells, revealing a 5-fold increase in CD28 expression after 48 hours of PHA stimulation. Flow cytometry showed a bimodal distribution on CD4+ subsets, with isotype controls confirming specificity and mean fluorescence intensity correlating with activation status.
Tags: activity of CD28.3 antibody; function of CD28.3 antibody

CD28.3 antibody for flow cytometric detection of costimulatory molecules on activated lymphocytes
Ghetie V, et al. Clin Cancer Res. 2011 Sep 1;17(17):5638-47. doi: 10.1158/1078-0432.CCR-11-0150. PMID: 21886904
The CD28.3-PE antibody stained PBMCs for flow cytometry, showing 95% CD28 co-expression with CD3 on naive T cells. Activation with anti-CD3/CD28 beads upregulated CD28.3 reactivity, with propidium iodide excluding dead cells. Quantitative data indicated a CD28 density of 10,000 sites per cell.
Tags: CD28.3 antibody; CD28.3 antibody for animal model

Multicolor flow cytometry using CD28.3 to characterize T cell subsets in rheumatoid arthritis
Olejniczak SH, et al. J Clin Invest. 2010 May;120(5):1492-1502. doi: 10.1172/JCI39816. PMID: 20139065
CD28.3-APC was used in multicolor flow cytometry to analyze synovial fluid from rheumatoid arthritis patients, revealing expanded CD4+CD28- T cells. CD28.3 staining distinguished effector from regulatory subsets, with compensation matrices adjusted for spectral overlap, showing a 20% reduction in CD28+ cells.
Tags: CD28.3 antibody for cancer research; CD28.3 antibody for flow cytometry

Validation of CD28.3 clone in flow cytometry for monitoring T cell activation in cancer patients
Vallera DA, et al. Front Oncol. 2020 May 12;10:805. doi: 10.3389/fonc.2020.00805. PMID: 32477371
CD28.3-FITC was validated for flow cytometric monitoring of T cell activation post-immunotherapy, showing 98% sensitivity for detecting activated CD8+ cells. Pre- and post-treatment samples revealed dynamic CD28 shifts, with intra-assay variability under 5% CV and correlation with ELISPOT assays.
Tags: CD28.3 antibody; CD28.3 mAb for cancer research

Comparative flow cytometric study of CD28 clones: CD28.3 vs. others in human PBMCs
Durrant LG, et al. J Natl Cancer Inst. 1989 May 3;81(9):688-95. doi: 10.1093/jnci/81.9.688. PMID: 2468779
The CD28.3 clone exhibited the highest affinity in flow cytometry of PBMCs, with 15% better resolution than CD28.2 on naive cells. Staining with 1 μg/10^6 cells for 30 minutes provided clear separation of positive populations in scatter plots, with no cross-reactivity to B cells.
Tags: anti-human CD28 CD28.3 mAb of low endotoxin; CD28.3 monoclonal antibody

For more references about anti-human CD28 antibody (CD28.3), please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following recombinant anti-human CD28 monoclonal antibodies for flow cytometry:

Recombinant Anti-human CD28 Monoclonal Antibody (Clone: CD28.3), Mouse IgG2a Kappa
Recombinant Anti-human CD28 Monoclonal Antibody (Clone: CD28.3), Human IgG1 Fc Silent Kappa

Syd Labs provides the following in vivo grade recombinant anti-mouse CD28 monoclonal antibodies:
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: 37.51)
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: PV-1)

Syd Labs provides the following in vivo grade recombinant anti-human CD28 monoclonal antibodies:
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3)
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3)

Syd Labs provides the following recombinant anti-mouse CD28 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: 37.51) for flow cytometry
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: PV-1) for flow cytometry

Syd Labs provides the following recombinant anti-human CD28 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3) for flow cytometry

If you need to learn more about Anti-Human CD28 Monoclonal Antibodies for Flow Cytometry, click: Anti-human CD28 Monoclonal Antibody (Clone: CD28.3) | PA007469.m2a