PA007475.m1: In vivo Grade Recombinant Anti-mouse CD28 Monoclonal Antibody, Mouse IgG1 Kappa (Clone: 37.51)
Recombinant Mouse IgG1 Monoclonal Antibody.
Clone: 37.51.
Isotype: Mouse IgG1 Kappa.
Source: The anti-mouse CD28 monoclonal antibody (clone: 37.51) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 37.51) specifically binds to the mouse CD28 protein.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD28 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
PA007475.hs2: In vivo Grade Recombinant Anti-mouse CD28 Monoclonal Antibody, Syrian Hamster IgG2 (Clone: 37.51)
Recombinant Syrian Hamster IgG2 Monoclonal Antibody.
Clone: 37.51.
Isotype: Syrian Hamster IgG2.
Source: The anti-mouse CD28 monoclonal antibody (clone: 37.51) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant hamster monoclonal antibody (clone: 37.51) specifically binds to the mouse CD28 protein.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD28 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD28 monoclonal antibody (clone: 37.51) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
References of anti-mouse CD28 antibody (37.51)
CD28 ligation induces transplantation tolerance by IFN-gamma-dependent depletion of T cells that recognize alloantigens.
Yu XZ, et al. (2004). J Clin Invest. 2004 Jun;113(11):1624-30. doi: 10.1172/JCI20940. PMID: 15173889
CD28-specific Ab (37.51) has been used in experimental models to mimic the natural ligands and provide costimulatory signals to T cells, thus preventing T cell anergy in vitro (2). Administration of anti-CD28 mAb 37.51 inhibits donor T cell expansion and prevents GVHD in mice (20). To test whether anti-CD28 treatment would prevent GVHD mediated by CD4 and CD8 T cells, a well-established murine GVHD model with C57BL/6 (B6) (H2 b ) donors and B10.BR (H2 k ) recipients was used. Anti-CD28 treatment significantly reduced GVHD lethality compared with control treatment ( P < 0.0001). In allogeneic BALB/c recipients, both WT and CD28-deficient donor (H2 b+ /CD8 + ) cells divided rapidly and expanded extensively, but there was a dramatic difference in the percentage of WT cells with or without anti-CD28 treatment.
Tags: anti-mouse CD28; anti-mouse CD28 37.51 antibody
Therapeutic effect of anti-OX40L and anti-TNF-alpha MAbs in a murine model of chronic colitis.
Totsuka T, et al. (2003). Am J Physiol Gastrointest Liver Physiol. 2003 Apr;284(4):G595-603. doi: 10.1152/ajpgi.00450.2002. PMID: 12631559
Administration of anti-OX40L MAb markedly ameliorated the clinical and histopathological disease in preventive and therapeutic protocols. In vivo treatment with anti-OX40L MAb decreased CD4+ T cell infiltration in the colon and suppressed IFN-γ, IL-2, and TNF-α production by lamina propria CD4+ T cells. The combination with anti-TNF-α MAb further improved the therapeutic effect by abolishing IFN-γ, IL-2, and TNF-α production by lamina propria CD4+ T cells. Histological examination showed prominent epithelial hyperplasia with glandular elongation with a massive infiltration of mononuclear cells in the lamina propria of the colon from the control IgG-treated mice. CD4+ LPL were isolated from the control rat IgG- or anti-OX40L MAb-treated mice at 7 wk after CD4+ CD45RB high T cell transfer and stimulated with anti-CD3 and anti-CD28 MAbs for 48 h.
Tags: anti-mouse CD28 37.51 in vivo; anti-mouse CD28 37.51 in animal model
B7-1 engagement of cytotoxic T lymphocyte antigen 4 inhibits T cell activation in the absence of CD28.
Fallarino F, et al. (1998). J Exp Med. 1998 Jul 6;188(1):205-10. doi: 10.1084/jem.188.1.205. PMID: 9653097
Primed T cells from both types of mice produced cytokines and proliferated in response to stimulator cells lacking B7 expression. However, whereas the response of CD28 1 / 1 T cells was augmented by costimulation with B7-1, the response of the CD28 2 / 2 T cells was strongly inhibited. This inhibition was reversed by monoclonal antibody against B7-1 or CTLA4. To determine whether expression of CTLA4 could indeed be induced in 2C/Rag/CD28 2 / 2 T cells, flow cytometric analysis was performed after restimulation in vitro with P815 cells. As shown in Fig. 2 A, primed CD28 1 / 1 T cells proliferated in response to P815, with a peak response observed on day 2.
Tags: anti-mouse CD28 37.51 in cancer research; anti-mouse CD28 37.51 in mouse tumor model
Signaling via CD28 costimulates lymphokine production, but does not reverse unresponsiveness to interleukin-2 in anti-CD3 triggered Th1 cells.
Sommer F, et al. (1993). Eur J Immunol. 1993 Oct;23(10):2498-502. doi: 10.1002/eji.1830231018. PMID: 8405051
The mAb 37.51(anti-mouse CD28 mAb), directed against murine CD28, was purchased from.... Cells (1 x 10^6) were incubated with mAb 37.51 (1 pg/ml) or an irrelevant hamster mAb for 30 min on ice in 100 pl PBS. All cell proliferation tests were performed in 96-well flat-bottom microtiter plates (Nunc, Wiesbaden, FRG), using Click’s/RPMI medium containing 10% FCS and supplements, as described elsewhere. In a first set of experiments, we tested for the expression of CD28 on the Th1 cells used in this study. Next, we tested whether stimulation with anti-CD28 mAb changed the reactivity of Th1 cells blocked by treatment with solid-phase-coupled anti-CD3.
Tags: function of anti-mouse CD28 37.51; bioactivity of anti-mouse CD28 37.51
CD28-induced costimulation of T helper type 2 cells mediated by induction of responsiveness to interleukin 4.
McArthur JG, et al. (1993). J Exp Med. 1993 Nov 1;178(5):1645-53. doi: 10.1084/jem.178.5.1645. PMID: 8228811
The mAb 37.51, directed against murine CD28, was used to provide costimulatory signals to Th2 cells. CD28 ligation enhanced IL-4 responsiveness in Th2 cells. In vivo administration of anti-CD28 mAb augmented Th2 responses in mouse models. The combination of anti-CD3 and anti-CD28 mAb induced high levels of IL-4 production. This costimulation was essential for the development of Th2 phenotype in vivo.
Tags: anti-mouse CD28 37.51 of low endotoxin; anti-mouse CD28 antibody (clone 37.51)
For more references about anti-mouse CD28 antibody (37.51), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-mouse CD28 monoclonal antibodies:
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: PV-1)
Syd Labs provides the following in vivo grade recombinant anti-human CD28 monoclonal antibodies:
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3)
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3)
Syd Labs provides the following recombinant anti-human CD28 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3) for flow cytometry