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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
|---|
Recombinant Mouse IgG1 isotype controls and Recombinant Hamster IgG2 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG1 Monoclonal Antibody.
Clone: 37.51.
Isotype: Mouse IgG1 Kappa.
Source: The anti-mouse CD28 monoclonal antibody (clone: 37.51) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 37.51) specifically binds to the mouse CD28 protein.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD28 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Recombinant Syrian Hamster IgG2 Monoclonal Antibody.
Clone: 37.51.
Isotype: Syrian Hamster IgG2.
Source: The anti-mouse CD28 monoclonal antibody (clone: 37.51) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant hamster monoclonal antibody (clone: 37.51) specifically binds to the mouse CD28 protein.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD28 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD28 monoclonal antibody (clone: 37.51) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Roles of lymphatic endothelial cells expressing peripheral tissue antigens in CD4 T-cell tolerance induction
Rouhani, S. J., et al. Nat Commun. 2015 Apr 10;6:6771. doi: 10.1038/ncomms7771. PMID: 25857745
However, Bg2 cells adoptively transferred into MHC-II−/−→Prox1xβ-gal chimeras treated with αCD28 agonistic antibodies also did not proliferate. ...CTV-labelled Bg2 cells were adoptively transferred into B6 and MHC-II−/−→Prox1xβ-gal mice treated with PBS, αCD28 or IFN-γ, and proliferation was analysed 3 days later. Plots are gated on Thy1.1+CD4+ cells. Data representative of 2 experiments with 2 mice each. ...CD4 co-cultures also received 0.25 μg ml−1 αCD28 (clone 37.51). ...Mice were irradiated (6.5 Gy × 2) and reconstituted with a minimum of 2 × 106 bone marrow cells depleted of CD4+ and CD8+ T cells (Miltenyi Biotec) to prevent graft-versus-host disease. ...Single colour images of LAMP1 and cathepsin L staining were imported into ImageJ and thresholded to identify positive vesicles as greater than 10 pixels in area.
Tags: anti-mouse CD28 37.51; anti-mouse CD28 37.51 in vivo
Multiple layers of CD80/86-dependent costimulatory activity regulate primary, memory, and secondary lymphocytic choriomeningitis virus-specific T cell immunity
Eberlein, J., et al. J Virol. 2012 Feb;86(4):1955-70. doi: 10.1128/JVI.05949-11. PMID: 22156513
A case in point is the costimulatory triad of CD28, CD80 (B7.1), and CD86 (B7.2), the role of which has been explored in numerous experimental settings. ...To date, infections with multiple distinct and related viruses, escalating dosages, and various challenge routes have been employed to ascertain the role of CD28-CD80/86 costimulation preferentially in CD28−/− mice but also complemented by analyses of CD80−/− and/or CD86−/− strains as well as ligand (anti-CD80/86 and CTLA-4Ig) and receptor (anti-CD28 and anti-CTLA-4) blockade. ...For the third paradigm, as reviewed previously by Boesteanu and Katsikis (9), the development of the concept that secondary TE responses are less dependent on CD28-CD80/86 costimulation than primary TE responses was based in large part on in vitro experiments. ...Although a similar interpretation was proposed for some nonviral model systems (50), this observation is also compatible with the notion of an activating CD80/86 receptor other than CTLA-4 or CD28. ...It should be noted that the impaired proliferative expansion of CD28−/− secondary CD8+ TE was also observed in experiments in which wt and CD28−/− CD8+ TM were transferred into separate recipients (not shown), thus ruling out a competitive disadvantage of CD28−/− T cells in the combined AT/rechallenge experiments described above.
Tags: anti-mouse CD28 37.51 in animal model; anti-mouse CD28 37.51 mAb in animal model
For more references about Anti-mouse CD28 Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-mouse CD28 monoclonal antibodies:
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: PV-1)
Syd Labs provides the following in vivo grade recombinant anti-human CD28 monoclonal antibodies:
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3)
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3)
Syd Labs provides the following recombinant anti-human CD28 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3) for flow cytometry
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