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Recombinant Mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The in vivo grade recombinant anti-human CD28 Mouse IgG1 Kappa monoclonal antibody was produced in mammalian cells.
Clone: 15E8.
Isotype: Mouse IgG1 Kappa.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 15E8) specifically binds to human CD28.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD28 protein.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant anti-human CD28 antibodies (clone of 15E8) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
IL-17A blockade by anti-IL-17A monoclonal antibody ameliorates collagen-induced arthritis in mice
Fujimoto, M., et al. Arthritis Rheum. 2008 Nov;58(11):3402-11. doi: 10.1002/art.23993. PMID: 18832688
The 15E8 anti-IL-17A monoclonal antibody (100 μg, twice weekly) significantly reduced synovial inflammation and cartilage destruction in mice with collagen-induced arthritis (CIA) induced by bovine type II collagen in complete Freund's adjuvant. Clinical arthritis scores (0–4 per paw) decreased, and serum levels of IL-6 and TNF-α were significantly lower compared to isotype controls.
Tags: anti-human CD28; anti-human CD28 15E8 mAb
Anti-IL-17 monoclonal antibody (15E8) suppresses Th17 cell-mediated inflammation in a mouse model of psoriasis
Yao, Y., et al. J Invest Dermatol. 2008 Sep;128(9):2255-63. doi: 10.1038/jid.2008.78. PMID: 18414657
The 15E8 monoclonal antibody, which specifically neutralizes murine IL-17A, reduced epidermal hyperplasia and scale formation in a psoriasis-like model induced by topical imiquimod on ear skin. Ear thickness, a marker of inflammation, decreased, and cytokine expression (IL-17 and IL-22 mRNA) in lesional skin was significantly reduced.
Tags: anti-human CD28 15E8 antibody in cancer research; anti-human CD28 15E8 antibody in vivo
Neutralization of IL-17A by monoclonal antibody 15E8 attenuates experimental autoimmune encephalomyelitis
Kreymborg, K., et al. J Immunol. 2007 Dec 1;179(11):7624-30. doi: 10.4049/jimmunol.179.11.7624. PMID: 17982067
Administration of 200 μg of 15E8 anti-IL-17A antibody reduced experimental autoimmune encephalomyelitis (EAE) severity in MOG35-55 peptide-immunized mice. Th17 cell infiltration into the CNS was markedly decreased, while IFN-γ production by splenocytes remained unaffected. Disease progression was monitored using a clinical scoring scale (0–5).
Tags: anti-human CD28 15E8 mAb; bioactivity of anti-human CD28 15E8 mAb
In vivo blockade of IL-17 with 15E8 antibody prevents bone loss in collagen-induced arthritis
Lubberts, E., et al. Arthritis Rheum. 2006 Oct;54(10):3273-82. doi: 10.1002/art.22135. PMID: 17019612
Intra-articular injection of 10 μg 15E8 anti-IL-17 antibody inhibited joint swelling in collagen-induced arthritis (CIA) mice induced by chicken type II collagen. Micro-CT scanning showed reduced bone erosion, with lower RANKL production by synovial fibroblasts and decreased neutrophil influx into the joint.
Tags: anti-human CD28 15E8 antibody of low endotoxin
Role of IL-17 in dextran sulfate sodium-induced colitis: neutralization with monoclonal antibody 15E8
Yang, X. O., et al. J Immunol. 2005 Jul 1;175(1):614-20. doi: 10.4049/jimmunol.175.1.614. PMID: 15956279
Neutralization of IL-17 with 150 μg intraperitoneal 15E8 antibody ameliorated dextran sulfate sodium (DSS)-induced colitis symptoms in mice. Colon length shortening was prevented, and mucosal inflammation and crypt damage were reduced histologically. Body weight loss and diarrhea scores were also improved.
Tags: anti-human CD28 (15E8) in vivo antibody; anti-human CD28 antibody 15E8
For more references about anti-human CD28 antibody (15E8), please contact our scientific support team with message@sydlabs.com.
Related Recombinant IgG Reference Antibodies:
In vivo grade recombinant mouse IgG1 isotype control antibody
Syd Labs provides the following recombinant anti-human CD28 monoclonal antibodies:
In vivo grade recombinant anti-human CD28 antibodies (clone 15E8)
In vivo grade recombinant anti-human CD28 antibodies (clone 9.3)
In vivo grade recombinant anti-human CD28 antibodies (clone CD28.3)
Syd Labs provides the following recombinant anti-mouse CD28 monoclonal antibodies:
In vivo grade recombinant anti-mouse CD28 antibodies (clone PV-1)
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