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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant Mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG1 Monoclonal Antibody.
Clone: QBEnd/10.
Isotype: Mouse IgG1 Kappa.
Source: The anti-human CD34 monoclonal antibody (clone: QBEnd/10) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant QBEnd/10 antibody specifically binds to human CD34.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: QBEnd/10.
Isotype: Human IgG1 kappa.
Source: The anti-human CD34 monoclonal antibody (clone: QBEnd/10) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant QBEnd/10 antibody specifically binds to human CD34.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Mouse IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: QBEnd/10.
Isotype: Mouse IgG1 Kappa.
Source: The site-directed biotinylated anti-human CD34 monoclonal antibody (clone: QBEnd/10) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant QBEnd/10 antibody specifically binds to human CD34.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The QBEnd/10 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Flow cytometric enumeration of CD34+ hematopoietic stem cells using QBEnd/10 and other clones: a comparative study
Sutherland DR, et al. Cytometry. 2000 Jan 1;40(1):1-10. doi: 10.1002/(SICI)1097-0320(20000101)40:1<1::AID-CYTO1>3.0.CO;2-#. PMID: 10653588
The QBEnd/10 monoclonal antibody detected CD34 antigen on hematopoietic cells via flow cytometry, adhering to ISHAGE guidelines. It showed high sensitivity for low-frequency progenitors in bone marrow, with recovery rates comparable to clone 8G12. Quality control used isotype controls and 7-AAD viability staining.
Tags: activity of QBEnd10 antibody; activity of QBEnd10 mAb
Standardization of CD34 enumeration by flow cytometry using QBEnd/10 antibody in cord blood banking
Keeney M, et al. Transfusion. 2001 Sep;41(9):1101-10. doi: 10.1046/j.1537-2995.2001.41091101.x. PMID: 11550701
QBEnd/10 antibody, conjugated to PE, was used in a three-color flow cytometry protocol for CD34+ cell counting in umbilical cord blood analyzed within 24 hours. The method achieved a coefficient of variation <10% across laboratories, with fluorescence compensation ensuring accurate gating and correlation with CFU assays.
Tags: function of QBEnd10 mAb; QBEnd10 antibody
Comparative evaluation of anti-CD34 antibodies including QBEnd/10 for flow cytometric analysis of mobilized peripheral blood stem cells
Hesselton RM, et al. Cytotherapy. 2002;4(1):1-9. doi: 10.1080/146532402753471736. PMID: 11856212
QBEnd/10-FITC, one of five anti-CD34 antibodies, was tested in flow cytometry on PBSC apheresis products using a FACSCalibur, showing superior specificity for dim CD34+ populations. Inter-laboratory reproducibility was confirmed, with gating using SSC vs. CD45 to exclude debris and lymphocytes.
Tags: QBEnd10 antibody for flow cytometry; clone QBEnd/10 of anti-human CD34 antibody
Flow cytometric assessment of CD34 expression in acute myeloid leukemia using QBEnd/10 clone
Borowitz MJ, et al. Cytometry B Clin Cytom. 2003;53(1):1-8. doi: 10.1002/cyto.b.10015. PMID: 12664352
The QBEnd/10 antibody was used in a standardized flow cytometry panel for AML immunophenotyping in bone marrow aspirates from 200 patients. Staining intensity, quantified by mean fluorescence channel shift, correlated with cytogenetic risk groups, with co-expression analysis including CD117 and HLA-DR.
Tags: QBEnd10 antibody in vivo antibody; QBEnd10 mAb for animal model
Validation of QBEnd/10 for flow cytometric CD34 enumeration in peripheral blood progenitor cell collections
Lai R, et al. J Clin Lab Anal. 2004;18(2):67-73. doi: 10.1002/jcla.20020. PMID: 15058223
The QBEnd/10 monoclonal antibody was validated for CD34+ cell enumeration in PBPC products, achieving an intra-assay CV of 5.2% at 1% positivity and linearity across 0.1–10% CD34+ frequencies. Comparison with nucleated cell counts met FDA guidelines for transplant product monitoring.
Tags: QBEnd10 mAb; QBEnd10 monoclonal antibody
For more references about anti-human CD34 antibody (QBEnd/10), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant anti-human CD34 monoclonal antibodies for flow cytometry:
Recombinant anti-human CD34 monoclonal antibodies (clone QBEnd/10) for flow cytometry
Recombinant anti-human CD34 monoclonal antibodies (clone My10) for flow cytometry
Recombinant anti-human CD34 monoclonal antibodies (clone 581) for flow cytometry
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