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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant mouse IgG2a monoclonal antibody.
Clone: 9.2.27.
Isotype: Mouse IgG2a kappa.
Source: The recombinant anti-human Neural/glial antigen 2 (NG2) monoclonal antibody was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant NG2 antibody specifically binds to the human NG2 protein.
Applications: Flow Cytometry (FC), immunocytochemistry (ICC/IF), immunoprecipitation (IP), and immunohistochemistry (IHC).
Form of Antibody: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The recombinant anti-human Neural/Glial Antigen 2 (NG2) monoclonal antibodies are shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
12 months from date of receipt, -20°C to -70°C as supplied.
Background
Neural/glial antigen 2 (NG2), also known as chondroitin sulfate proteoglycan 4 (CSPG4), melanoma chondroitin sulfate proteoglycan (MCSP), or high molecular weight melanoma-associated antigen (HMW-MAA), is a cell-surface transmembrane proteoglycan. Its approximately 250 kDa N-linked core glycoprotein can carry chondroitin sulfate chains, yielding a proteoglycan form exceeding 450 kDa. NG2/CSPG4 is expressed on oligodendrocyte precursor cells, glioma cells, and many malignant melanomas, as well as selected other tumors. It contributes to cell–substratum interactions and is involved in tumor cell proliferation, adhesion, migration, and invasion. NG2/CSPG4 also functions as a coreceptor for α4β1 integrin, making it a relevant target for studies of tumor biology and neural development.
The 9.2.27 monoclonal antibody specifically recognizes human NG2/CSPG4. The original clone was generated by immunizing BALB/c mice with an extract of human M14 melanoma cells and recognizes the core glycoprotein of the melanoma-associated chondroitin sulfate proteoglycan. Clone 9.2.27 has been used in flow cytometry, immunofluorescence/immunocytochemistry, immunoprecipitation, and immunohistochemistry to detect and characterize NG2/CSPG4-expressing cells. It has also been investigated in functional studies of melanoma cell interactions and tumor growth, providing a research tool for examining NG2/CSPG4 expression and its biological roles.
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