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BP002590-ENZ-376: Recombinant Human Malic Enzyme 2 (ME2)
Source: E. coli-derived.
ME2 activity was assayed spectrophotometrically at 340 nm as described in Mandela and Sauer (1975). The standard reaction mixture contained 50 mM Tris.HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate, Assay was performed in a Beckman spectrophotometer. The Km value is 1.5?.6 mM.
Purity: > 95.0% as determined by HPLC and SDS-PAGE.
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