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Conditions of optimal ME2 Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
ME2 catalyzes the oxidative decarboxylation of malate to pyruvate, malat + NAD(P)+ pyruvate + CO2 + NAD(P)H+, and is found both in eukaryotic and prokaryotic cells. Three different isoforms of ME are known to be in mammalian tissues: a strictly cytosolic NADP+-dependent enzyme, an NADP+-dependent mitochondriail isoform, and a mitochondrial isoenzyme that is able to use both NAD+ and NADP+ but is more effective with NAD+. The mammalian isoforms size is about 62-64 kDa. A native size of 240,000 Da proposes a tetrameric structure for the active enzyme.
Mitochondrial NAD+-dependent ME 2 activity is seen in tissues that experience many cell divisions, like spleen, thymus, and the basal cells of the small intestinal mucosa. ME2 is also expressed all through the rapid cleavage stages of early Xenopus development. Activity for this isoform is low or nonexistent in brain, muscle, and normal and regenerating liver tissue from rat but was observed in rat adrenal cortex, pigeon and human skeletal muscle, and in heart muscle of some species. In addition, it is expressed in mitochondria of all tumor cells inspected to detain ascites tumors, hepatoma cells, and a variety of other tumors and transformed cell lines.
BP002590-ENZ-376: Recombinant Human Malic Enzyme 2 (ME2)
Source: E. coli-derived.
A single, non-glycosylated polypeptide chain containing 573 amino acids and having a total molecular mass of 64.4 kDa.
ME2 activity was assayed spectrophotometrically at 340 nm as described in Mandela and Sauer (1975). The standard reaction mixture contained 50 mM Tris.HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate, Assay was performed in a Beckman spectrophotometer. The Km value is 1.5?.6 mM.
Purity: > 95.0% as determined by HPLC and SDS-PAGE.
Formulation: The protein was Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, 150 mM NaCl, 1 mM mercaptoethanol, 1 mM EDTA, pH8.0.
Reconstitution: It is recommended to reconstitute the lyophilized ME2 in sterile 18 M?-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
ME2 Antibodies:
ME2 Polyclonal Antibody
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