Our complete custom Adeno-associated Virus (AAV) production services include: 1) AAV plasmid vector construction; 2) AAV packaging; 3) AAV purification by iodixanol or column chromatography; 4) AAV titering by qPCR.
We have always optimized our AAV production systems, including AAV plasmid vector design and construction and low endotoxin plasmid preparation, host cells for AAV packaging, cell culture medium and AAV packaging process, small and large scale AAV purification process.
Fast turnaround: 2-3 weeks.We provide large selection of pre-made adenovirus and complete custom adenovirus production services from small crude scale to large purified scale, including: 1) construction of an adenovirus vector for expression or silencing of a gene; 2) generation of adenovirus; 3) amplification and purification of adenovirus.
Our adenovirus production team has constructed, packaged, amplified, and purified thousands of adenoviruses for worldwide clients in more than a decade. An optimized technology is used to simplify and accelerate adenoviral vector construction for researchers studying gene expression or gene silencing in mammalian systems.
Fast turnaround: 2-3 weeks.Our Ad5-RGD and Ad5/35 adenovirus production services include: 1) construction of an Ad5-RGD and Ad5/35 adenovirus vector for expression or silencing of a gene; 2) generation of Ad5-RGD and Ad5/35 adenovirus; 3) amplification and purification of Ad5-RGD and Ad5/35 adenovirus.
Our adenovirus production team has constructed, amplified, and purified thousands of adenoviruses for worldwide clients in more than a decade. An improved technology is used to simplify and accelerate construction of adenoviral vectors for researchers studying gene expression or gene silencing in mammalian systems.
Fast turnaround: 2 weeks.Our adenovirus production services for toxic genes include: 1) construction of an adenovirus vector for expression of a cytotoxic gene; 2) generation of adenoviruses carrying cytotoxic genes; 3) amplification and purification of adenoviruses carrying cytotoxic genes.
Our adenovirus production team has constructed, amplified, and purified thousands of adenoviruses for worldwide clients in more than a decade. An improved technology is used to simplify and accelerate construction of adenoviral vectors for researchers studying gene expression or gene silencing in mammalian systems.
Fast turnaround: 2 weeks.BP000861-ENZ-406: Recombinant Human AKR1C3 (Aldo-keto reductase family 1 member C3)
Source: E. coli-derived.
Specific activity: approximately < 0.1 units/mg.
Purity > 95%, by SDS-PAGE.Fast turnaround: 8-10 weeks in total. Our antibody humanization process involves significant computational analysis, and thus is quite efficient and requires much less trial time.
A human antibody database and a proprietary method are used for analysis and design of humanized antibodies.
Nine humanized antibodies (each combination of 3 designed heavy chains and 3 designed light chains) are constructed and produced for antigen binding confirmation and affinity measurement.
Fast turnaround: 3-6 months with our proprietary optimization algorithm.
Antibody affinity can be improved by about 10 folds after each round of antibody affinity maturation.
100000 cells per hybridoma clone are sufficient for cloning and sequencing the monoclonal antibody variable regions (both VH and VL) and full length. Welcome to challenge us with smaller number of clonal B cells or non-viable cells for hybridoma rescue.
- Reliable results. With optimized workflow and PCR primers, we have successfully cloned and sequenced >3000 antibodies from various species in the past 10 years. Syd Labs has become a reliable CRO supplier of antibody sequencing to biotech and biopharma companies, government agencies including FDA and NIH, and universities. Clients' samples of hybridoma cell lines or clonal B cells just need to meet 2 requirements: functional antibodies are secreted by the cells; enough RNA remains.
- Save time. One may learn how to clone and sequence antibody genes by studying hybridoma antibody cloning and antibody sequencing protocols that we collected from public literature. Generally speaking, it is more difficult to clone antibody genes from rodents than other species; among isotypes, it is the easiest to clone IgG genes, especially IgG1. A couple of companies sell such kits and PCR primers. However, it takes time to become a highly efficient and experienced professional for so many lab skills and bioinformatics analysis involved in such projects. In addition, there are some samples invaluable but difficult to work. Some service suppliers spend months but could not solve the problem, just wasting clients' samples and time.
- Save money. Since we process samples from multiple clients in the same time, our cost is lower than clients'. Our low cost molecular biology reagents further save clients' cost.Kinetic analysis is used to determine the affinity of an antibody to its soluble antigen using surface plasmon resonance (SPR) or biolayer interferometry (BLI), which can measure kon, koff, and kD. Association and dissociation are measured on the Biacore T100 or the ForteBio Octet QKe using various concentrations of the antigen and the kD is calculated using an appropriate curve-fitting model.
Fast turnaround time: 2-3 days per antibody-antigen pair, but may require more time depending on the complexity of analysis.BP000632-ENZ-488: Recombinant Human ASPH
Source: E. coli-derived.
Purity > 90%, by SDS-PAGE.