BP005344: Human IL-6 Protein with No Tag
Source: The recombinant Human IL-6 Protein, (amino acids Val30-Met212) fused with No Tag, was produced in E.coli cells.
Activity: Immobilized recombinant Human IL-6 at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human IL-6 R alpha, hFc tag with the EC50 6.0 ng/ml determined by ELISA The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.EK000015-EK0410: Human IL-6 ELISA Kit
Range 4.69pg/ml-300pg/ml
Sensitivity < 0.3pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitive detection of human IL-6 in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.EK000015-EK0411: Mouse IL-6 ELISA Kit
Range 15.6pg/ml-1000pg/ml
Sensitivity < 1 pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitive detection of mouse IL-6 in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.EK000015-EK0412: Rat IL-6 ELISA Kit
Range 62.5pg/ml-4000pg/ml
Sensitivity < 5pg/ml
Specificity: No detectable cross-reactivity with any other cytokine.
Application: For quantitative detection of rat IL-6 in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: Four months at 4°C and eight months at -20°C.BP000159-C009: Recombinant Human Interleukin-6 (IL-6) Protein
Source: E. coli-derived.
The ED50 as determined by the dose-dependant stimulation of human TF-1 cells is less then 0.1 ng/ml, corresponding to a Specific Activity of 5.0 x 107 IU/mg.
Purity: > 95% by RP-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 EU/ug).BP000159-CYT-213: Recombinant Human Interleukin-6 (IL-6) Protein
Source: E. coli-derived.
The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is less than 0.1 ug/ml, corresponding to the specific activity of 1.0 x 10,000,000 Units per mg.
Purity: > 97% by SDS-PAGE and RP-HPLC.BP000159-CYT-274: Recombinant Human Interleukin-6 (IL-6) Protein, CHO
Source: Chinese Hamster Ovarian Cells-derived.
The activity was measured in a cell prolifiration assay using B9 cells. The ED50 for this effect is 0.008-0.02 ug/ml corresponding to a Specific Activity of 50-125 MIU/mg.
Purity: > 97% by SDS-PAGE and RP-HPLC.BP000159-CYT-350: Recombinant Mouse Interleukin-6 (IL-6) Protein
Source: E. coli-derived.
The ED50 as determined by the dose-dependant stimulation of the proliferation of IL-6-dependent murine 7TD1 cells is < 0.02 ug/ml, corresponding to a specific activity of > 50,000,000 units/mg.
Purity: > 96% by SDS-PAGE and RP-HPLC.BP000159-CYT-388: Recombinant Rat Interleukin-6 (IL-6) Protein
Source: E. coli-derived.
The ED50 = 0.03-0.1 ug/ml corresponding to a specific activity of 10-34 MIU/mg. The biological activity is determined by measuring the dose-dependant proliferation of IL-6 dapendent B9 cells. A concentration range of 0.1 to 10.0 ug/ml is effective for most in vitro applications.
Purity: > 95% by SDS-PAGE and RP-HPLC.BP000159-CYT-098: Recombinant Human Interleukin-6 (IL-6) Protein, HEK
Source: HEK-derived.
The activity was determined by the dose dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) and is typically 0.2-1 ng/ml.
Purity: > 95% as obsereved by SDS-PAGE.